Mac 11: R Flashcards
Match each item to a choice
Match the process in A with the enzyme in B
- Unwinds the double stranded DNA
- Copies the DNA using each strand as a template for the newly synthesized DNA strand
- Okazaki fragments
- Copies the DNA using each strand as a template for the newly synthesized RNA strand
- RNA Polymerase
- DNA Polymerase
- DNA Ligase
- Helicase
- Helicase
- DNA Polymerase
- DNA Ligase
- RNA Polymerase
CRISPR is an abbreviation for
- Clustered Regularly Interspersed Short Palindromic Repeats
- Clustered Regularly Interspaced Short Palindromic Repeats
- Conservatory Regularly Interspersed Short Palindromic Repeats
- Conservatory Regularly Interspaced Short Palindromic Repeats
-Clustered Regularly Interspaced Short Palindromic Repeats
Match each item to a choice
Match item A with item B
- Start site of transcription
- Synthesizes the aminoacyl t-RNA
- Makes the peptide bond between two amino acids
- Start codon
- Peptidyl Transferase
- Promoter
- AUG
- Aminoacyl Trna Synthetase
- Promoter
- Aminoacyl Trna Synthetase
- Peptidyl Transferase
- AUG
Arrange the following
Arrange the following steps in the correct order as they are performed in a PCR test:
- subject the DNA product to gel electrophoresis
- Raise temperature of thermal cycler to 75 deg C, repeat cycle
- Mix nucleotides, primers, Taq polymerase and buffer in tube and load into thermal cycler
- Raise temperature to 95 deg C
- Lower temperature to 55 deg C
-3, 4, 5, 2, 1
True or False
In the Honolulu technique for cloning animals, electric shock is used to fuse the donor cell with the enucleated cell and stimulate cell development.
-False
Replication of DNA into daughter DNA molecules is described as
- Conservative
- Semi-Conservative
- Dispersive
- End to End
-Semi-Conservative
Fill in the blanks
The gene of interest (foreign DNA) is integrated into a plasmid or phage vector and this is referred to as _____ (two words). _____ is necessary to make numerous copies of the DNA to have enough DNA to insert into host cells. The pure colony containing the desired DNA is then chosen and cultured to harvest large amounts of recombinant proteins.
- Recombinant DNA
- Cloning
DNA is found in these organelles in the cell
- Nucleus and Mitochondria
- Mitochondria and Cytoplasm
- Golgi Apparatus and Rough Endoplasmic Reticulum
- Nucleus and Cytoplasm
-Nucleus and Mitochondria
