Macro/Micro Morphology Flashcards
What is the macroscopic morphology of Klebsiella pneumoniae?
Large moist gray colonies
What is the macroscopic morphology of Escherichia coli on EMB and MAC agars?
Green metallic sheen on EMB
Dry pink colonies w halo on MAC
What is the microscopic morphology of Klebsiella pneumoniae?
Gram negative coccobacilli
Straight rods
What makes Klebsiella macroscopic morphology different from other Enterobacteriaceae?
Klebsiella produces mucoid colonies
What organism produces a burnt chocolate odor on SBA?
P. mirabilis/P. vulgaris
What is the colony morphology of Salmonella spp?
Clear colorless colonies with black centers on MAC
Non lactose fermenter
What is the colony morphology of Y. pestis?
Grows best at 25C - 35C
Grows on SBA and MAC
What is the microscopic morphology of Y. pestis?
Gram negative, short, plump bacillus
“Safety” pin when stained with methylene blue
What do Kluyvera spp. resemble on a MAC agar?
E. coli colonies
What can identify E. coli O157:H7 and how does it appear?
MAC agar with sorbitol (SMAC)
Appears colorless
What is a selective agar for Vibrio spp?
TCBS
Vibrio spp thrive in brackish/marine water
What do V. mimicus, parahaemolyticus, P. damsela, and mot V. vulnificus produce?
Green colonies on TCBS
What do V. cholera, V. alginolyticus, and V. fluvalis produce?
Yellow colonies on TCBS
What is the microscopic morphology of Campylobacter spp?
Long spirals or ‘S’ seagull wing shape
Non spore forming, gram negative rods
What is the microscopic morphology of Acinetobacter spp?
Gram negative coccobacilli
Gram negative cocci
Can appear as gram positive cocci
What are the selective media used for the recovery of Burkholderia cepacia?
PC
OFPBL
BCSA
Oxidase
What are the selective media used to identify Enterobacteraciae members?
MAC
EMB
HEK
XLD
