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Flashcards in Mass spec Deck (8)
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1

What is Trypsinization?

Digestion of protein by trypsin

2

What is peptide map fingerprints?

peptides unique for a protein

3

How does mass spectrometer separate ions?

mass/charge ratio

Mass constant, different charges

Single charged, doubly charged, triple charged

4

The degree of deflection depends on what?

Molecular mass of ions (m/z ratio)

Smaller gets deflected more (requires less force)

Bigger gets deflected less

5

What is ESI-MS?

knocks out electrons making protein positively charged

Proteins with the most charge reaches detector first while least charge will arive last (most + AA which are Cys, Thr, Arg)

6

What is MALDI-MS?

treat with acid which will give protons

all +1 charge with different masses

Since all have +1 charge, the m/z ratio will start from the smallest to the largest

7

What is the tallest peak called?

base peak

8

What is the rightmost peak called?

parent peak