MB 351 - Lectures 9, 10, 11 Flashcards Preview

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Flashcards in MB 351 - Lectures 9, 10, 11 Deck (32):
1

Atypical Cell Walls: -waxy lipid bound to peptidoglycan -what is this waxy lipid? and what kind of bacteria have this?

-Acid fast cell wall type -waxy lipid is mycolic acid -prevents the uptake of most dyes -mycobacterium and nocardia

2

Most bacteria are either....think type of cell wall

gram negative or gram positive

3

Atypical Cell Walls: -Lack cell walls

Mycoplasmas and Archaea

4

Mycoplasmas

-lack cell walls -sterols in plasma membrane (lipids that protect from lysis)

5

Archaea

-Wall-less or -Walls of pseudomurein (lack NAM and D-amino acids)

6

Why do microorganisms move, and what is the movement called?

Tactic (taxis) response behavior -looking for nutrients (C&N) -avoiding predators -catching prey

7

Stimuli that microbes move in response to: three of them

aerotaxis - move towards oxygen concentration phototaxis - photon intensity chemotaxis - chemicals that attract or repel microbes

8

Peritrichous

flagella all over cell

9

monotrichous and polar

single flagella

10

lophotrichous and polar

a tuft of flagella coming from one pole

11

amphitrichous and polar

flagella on both poles of the cell

12

Various patterns of motility: RUNS

a run or swim is when a bacterium moves in one direction for a length of time. Runes are interrupted by tumbles. Then a run resumes. Tumbles are caused by reversal of the flagellar rotation

13

Some bacteria with polar flagella will have reversible motors that allow them to move forward when it turns ______ and backwards when it goes ______.

CCW, CW -some are incapable of this, and may only travel in one direction (only CW flagella = unidirectional). They must stop, reorient in order to change directions

14

Flagella (gram -) three basic parts

-filament made of flagellin proteins -wider hook -basal body, which anchors the flagellum to the cell wall and plasma membrane

15

The basal body contains a small central rod inserted into a series of rings. What are these rings and where are they located?

-L ring is embedded in the OM and LPS -P ring is in the peptidoglycan -MS and C rings are embedded in the cytoplasmic membrane

16

Which proteins function as the flagellar motor?

Mot proteins (bound to MS and C ring)

17

Fli proteins function as?

function as the motor switch

18

A __________ model has been proposed to explain rotation of flagellum.

proton turbine. Protons flowing thru the Mot proteins may exert forces on charges present on the C and MS rings, thereby spinning the rotor and the attached filament. 20,000 rpm

19

-occur at the poles or can be evenly distributed over surface of cell -hairlike appendages that are shorter, straighter, and thinner than flagella -tend to adhere to each other and to surfaces, as a result, they are involved in forming _______

Fimbriae - also help bacteria to adhere to epithelial surfaces in the body biofilms

20

Pili

-usually longer than fimbriae and number fewer only 1-2 per cell -Pili are involved in DNA transfer -- connect two cells for the exchange of genetic material -also aid in the attachment to host cells during infection

21

Pili involved in this kind of motility ________ and _________

-Twitching motility a special "type IV" pilus extends by the addition of subunits of pilin, makes contact with a surface and then retracts as the pilin subunits are disassembled. -called GRAPPLING HOOK MODEL (short, jerky, intermittent movements) -GLIDING motility - a smooth gliding movement of myxobacteria. Provides a means for microbes to travel in environments with low water content, such as biofilms and soil.

22

Prokaryotic cells lack: Also, cytoskeleton proteins are _______ to eukaryotes.

nucleus, no carbohydrates and usually lack sterols in their plasma membrane -Sterols are essential in all eukaryotic cell membranes. They modulate membrane fluidity and permeability. Sterols and carbs serve as receptors present in eukaryotes. SIMILAR

23

Plasma membrane contains

phospholipid bilayer with lipid-soluble proteins

24

Due to lack of ________ in the plasma membrane of prokaryotes, they are less rigid than eukaryotic membranes.

sterols

25

Phospholipid molecules contain:

polar head made of phosphate and glycerol that is hydrophilic (soluble in water), and nonpolar tails composed of fatty acids that are hydrophobic (insoluble in water)

26

Fluid mosaic model

phospholipids and proteins (reception and transport proteins) are not static, but move quite freely

27

Functions of the plasma membrane: three things

selective permeability, protein anchor, energy generating/conservation

28

Selective permeability

Most important function of the cell membrane is to serve as a selective barrier through which materials enter and exit the cell. Only certain molecules and ions can pass through the membrane. This function allows the cell to concentrate certain metabolites and excrete waste materials.

29

Protein anchor

Plasma membrane serves as the protein anchor, some proteins anchor together different layers of the cell envelope providing structure support. (Carrier proteins). Anchors for enzymes (ATP production, transport proteins).

30

Energy conservation

microbial energy generating processes involve the generation of a PROTON MOTIVE FORCE (PMF) where protons are selectively moved across the membrane.

31

Membrane Permeability Chart 

A image thumb
32

Enzymes made within the cell, then released outside of the cell to begin the process of extracellular digestion. Used since large macromolecules cannot normally pass through the cell. These _______ can be classified on the basis of what kinds of molecules they hydrolyze: 

Exoenzymes

hydrolyze: carbohydrates, lipids, proteins, nucleic acids 

ex. amylase hydrolyzes starch into mono- and dissacharide subunits. Iodine binds to starch, but not to its breakdown products (clear streak)