med sig Flashcards by Christianne Espiña

catalase positive, grow in BAP as creamy, white, pinhead colonies
Grows in MSA and ferments mannitol (yellow colonies)

S. aureus

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Grows in MSA but cannot ferment mannitol
susceptible to novobiocin
bacterial endocarditis after prosthetic heart valve insertion

S. epidermidis

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resistant to novobiocin
UTI among sexually active young females

S. saprophyticus

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PYRase positive
sensitive to 0.04U Bacitracin (Taxo A)
Sulfamethoxazole-Trimethoprin resistant

S. pyogenes

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Beta hemolytic in BAP
CAMP (Christie-Atkins-Munch-Peterson) positive
Hippurate hydrolysis positive
Neonatal meningitis

S. agalactiae

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(+) bile esculin test
(+) 6.5% salt tolerance test
(+) PYRase
grows at 10℃ and at 45℃

Enterococcus

Group D

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lancet or bullet shaped
Alpha hemolytic
Lobar pneumonia and meningitis

S. pneumoniae

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(+) Mouse inoculation test
(+) inulin fermentation
(+) bile solubility test
(+) optochin susceptibility
(+) Neufeld Quellung test positive

S. pneumoniae

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dental carries and subacute bacterial endocarditis

Viridans

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(-) Mouse inoculation test
(-) inulin fermentation
(-) bile solubility test
(-) optochin susceptibility
(-) Neufeld Quellung test positive

Viridans

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What does nutritionally variant streptococci requires?

vitamin B6 (pyridoxal) and thiol (cysteine)

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What causes opthalmia neonatorium?

N. gonorrhoeae

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Carbohydrate utilization test result: uses glucose

N. gonorrhoeae

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Waterhouse Friedrichsen Syndrome

N. meningitidis

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morphologically similar to Neisseria but asaccharolytic and DNAse positive

Moraxella catarrhalis

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pseudomembrane in throat

C. diphtheriae

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assoc. with spontaneous abortion

L. monocytogenes

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tumbling end-over-end motility
umbrella-shaped or inverted Christmas tree pattern

(+) Bile esculin hydrolysis
(+) Catalase
(+) CAMP test
(+) Voges-Proskauer positive

L. monocytogenes

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specific test for L. monocytogenes

Ocular test of Anton

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butcher’s disease
seal finger
whale finger
erysipeloid

E. rhusiopathiae

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long, thin, branching, gram positive bacilli; partially acid fast

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Nocardia

Grows in MacConkey and EMB
TSIA = A/A with gas
IMVC ++00
LOA ++0
motile

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E. coli

Growns in MacConkey
TSIA = A/A with gas
Non motile
IMVC 00++
LOA +00
weakly urease positive

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K. pneumoniae

What test differentiates K. pneumoniae and K. oxytoca?

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K. oxytoca is indole (+)

- Produces red pigment prodigiosin; agent of red milk - Lipase positive, gelatinase positive, **DNase positive** | DNase is what differentiates it from other Enterobac

Serratia marcesens

- IMVC ++0 - **H2S positive** - LOA ++0 - TSIA = K/A

Edwardsiella tarda

specific test for Salmonella

Widal test

- Resembles Salmonella - (+) ONPG positive - (-) Lysine decarboxylase

Citrobacter spp.

- Biologically inert - **non-motile** - bacillary dysentery - Grows in MacConkey and HEA - TSI = K/A

Shigella

- TSIA = K/A **with gas and H2S** - Motile - (+) phenylalanine deaminase - **strongly urease positive**

Proteus spp.

- Associated with plague - transmitted by flea - stalactite patterns; stained using Wayson stain

Yersinia pestis

Red bull’s eye colonies in CIN

Y. enterocolitica

- Grape-like odor, feather edges, produces pyocyanin and pyoverdin - motile, grows at 35 and 42℃ - TSIA = K/K - (+) oxidase - (+) **acetamide** utilitzation - (+) **cetrimide** positive

Pseudomonas aeruginosa

Glander's disease

B. mallei

melioidosis

B. pseudomallei

* pink eye conjunctivitis * requires X and V factor

H. aegyptius

* soft chancres * schools of fish in Gram stain * requires **X factor only**

H. ducreyi

* Pitting of agar * bleach like odor

Eikenella corodens

- Cultured in biphasic medium - Causes undulant fever, Malta fever

Brucella

specimen of choice for Bordetella pertussis

nasopharyngeal swab

- Cultured in BCYE - isolated in air conditioning towers and heating systems

Legionella pneumophila

- animal bites and scratches - Musty odor (odor of mushroom) - non-motile

Pasteurella multocida

- Grows at 42℃ - seagull wings appearance - darting motility - Cultured in Skirrow’s medium and Campy’s agar

Campylobacter

- Agent of bioterrorism; causes anthrax (cutaneous, pulmonary, gastrointestinal) - Medusa head colonies; bamboo pole or fishing rod appearance

Bacillus anthracis

Fried rice bacilli

Bacillus cereus

- Associated with **Risus sardonicus** - produces tetanospasmin - Drum stick appearance

Clostridium tetani

- Associated with **clindamycin associated-pseudomembranous colitis** - Produces **yellow colonies** on Cycloserine Cefoxitin Fructose Agar (**CCFA**)

C. difficile

- Double zones of hemolysis - (+) positive lecithinase - Nagler plate - (+) reverse camp - Causes gas gangrene or myonecrosis

C. perfringens

- Produces botulism toxin which is associated with **flaccid paralysis** - (+) Lipase

C. botulinum

Capnophilic and has gliding motility

Capnocytophaga

med sig Flashcards

(50 cards)