media

Question 1

as differential: allows differentiation of hemolysis

tryptic soy agar with 5% sheep blood

Answer 1

sheep blood agar

Question 2

as enrichment: supplies X (hemin) and V (NAD) factor

Answer 2

CHOC

Question 3

medias selective to gram (+)

Answer 3

Colistin and Nalidixic Agar (CNA) + Phenylethyl Alcohol agar (PEA)

Question 4

use for culturing streptococci, pneumococci, and meningococci

Answer 4

Brain Heart Infusion Agar

Question 5

as selective: isolates enteric gram (-) rods
as differential: lactose vs non-lactose fermenters

Answer 5

Eosin and Methylene Blue (EMB), MAC, and XLD agar

Question 6

color of lactose fermenters in EMB media

Answer 6

green-black or purple

E. coli: green metallic sheen

Question 7

color of lactose fermenters in MAC media

Answer 7

pink

Question 8

selective media for E. coli 0157:H7

Answer 8

Sorbitol MacConkey agar (SMAC)

E. coli 0157:H7 doesn’t ferment sorbitol = colorless!

Question 9

pH indicator of MAC and salmonella-shigella agar

Answer 9

neutral red

Question 10

inhibitory component of MAC

inhibits g (+)

Answer 10

bile salts and crystal violet

Question 11

pH indicator of Hektoen-Enteric Agar and Hugh-Leifson OF medium

Answer 11

bromthymol blue

Question 12

agars that have phenol red as indicator of pH

Answer 12

XLD, MSA, Urea, and TSI agar

Question 13

sugars present in XLD agar

Answer 13

• lactose
• sucrose
• xylose

Question 14

sugars present in HEA

Answer 14

• lactose
• sucrose
• salicin

Question 15

inhibitory component of XLD agar

Answer 15

sodium deoxycholate

Question 16

appearance of Shigella and Salmonella on HEA

Answer 16

green colonies

but Salmonella has black center

Question 17

E. coli on HEA and XLD

Answer 17

yellow colonies

Question 18

enrichment media for Campylobacter

Answer 18

Thioglycolate medium

incubation at 4℃

Question 19

transportation and growth media of Neisseria gonorrhoeae

Answer 19

JEMBEC plates

Question 20

differential medium for isolation of C. diphteriae

Answer 20

Cystine-tellurite blood agar and Tinsdale agar

Question 21

enrichment medium for C. diphtheriae

Answer 21

Loeffler medium

promotes development of metachromatic granules

Question 22

selective for Salmonella only!

Answer 22

Bismuth sulfite agar

Question 23

selective for Yersinia, Aeromonas, and P. shigelloides

Answer 23

Cefsulodin-Irgasan-Novobiocin (CIN) agar

Question 24

appears as red “bull’s eye” colonies surrounded by colorless halo on CIN agar

Answer 24

Y. enterocolitica

Question 25

**enrichment** for *Vibrio spp.*

Answer 25

Alkaline Peptone Water

Question 26

**selective** for *Vibrio spp*

Answer 26

TCBS agar

Question 27

yellow colonies on TCBS agar | ferments sucrose

Answer 27

*V. cholerae*

Question 28

appears like mercury droplets in Bordet-Gengou agar

Answer 28

*Bordetella*

Question 29

**potato**-glycerol-based medium enriched with blood | selective enrichment medium for *B. pertussis*

Answer 29

Bordet-Gengou agar

Question 30

charcoal agar with horse blood, cephalexin, and amphotericin B | selective for *B. pertussis*

Answer 30

Regan-Lowe agar

Question 31

**enrichment** for *Legionella* | has yeast extract and L-cysteine

Answer 31

BCYE agar

Question 32

component of Martin-Lewis that inhibits growth of yeast/mold

Answer 32

Anisomycin