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Introduction to Microbiology > Metabolic assays (Lab) > Flashcards

Metabolic assays (Lab) Flashcards

1
Q

What is a starch hydrolysis test used for?

A

To test for the presence of amylase and starch hydrolysis

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2
Q

What is an oxidative fermentation test used for?

A

To test if the organism is using oxidation or fermentation to metabolize glucose

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3
Q

What is a carbohydrate fermentation test used for?

A

If an organism can use a particular carbohydrate as an energy and carbon source

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4
Q

What is a methyl red (MR) test used for?

A

Tests if an organism is doing mixed acid fermentation

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5
Q

What is a Voges-Proskauer test used for?

A

Tests if an organism is doing butanediol fermentation

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6
Q

What is a citrate test used for?

A

Tests for the presence of citrate lyase and if the organism can use citrate for fermentation

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7
Q

What is a KOH test used for?

A

Confirms if an organism is gram-positive or gram-negative

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8
Q

What is an oxidase test used for?

A

Tests for the presence of cytochrome C

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9
Q

What is an ONPG test used for?

A

Tests for the presence of beta-galactosidase and lactose breakdown

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10
Q

What is an indole test used for?

A

Tests for the presence of tryptophanase and indole production

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11
Q

What is MacConkey agar used for?

A

Selects for enteric gram-negative bacteria and differentiates them based on lactose fermentation

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12
Q

What is EMB agar used for?

A

Selects for gram-negative bacteria and differentiates them based on fermentation type

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13
Q

What is a catalase test used for?

A

Detects the presence of catalase to differentiate between the Micrococcaceae and the Streptococcaceae

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14
Q

What is a nitrate reduction test used for?

A

Tests for the ability of an organism to do nitrate reduction

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15
Q

What is blood agar used for?

A

Used to grow fastidious microbes and tests for hemolysis and the production of hemolysins

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16
Q

What is mannitol salt agar used for?

A

Selects for organisms capable of tolerating high salt conditions and differentiates them based on mannitol fermentation

17
Q

What are the reagents used in a starch hydrolysis test? (2)

A
  1. Nutrient agar + starch

2. Iodine

18
Q

What are the reagents used in an oxidative fermentation test? (4)

A
  1. Bromothymol blue
  2. Glucose
  3. Peptone
  4. Mineral oil
19
Q

What are the reagents used in a carbohydrate fermentation test? (4)

A
  1. Durham tube
  2. Carbohydrate of interest
  3. Phenol red
  4. Peptone
20
Q

What are the reagents used in a methyl red test? (2)

A
  1. Methyl red

2. Nutrient broth

21
Q

What are the reagents used in a Voges-Proskauer test? (3)

A
  1. Nutrient broth
  2. KOH
  3. Alpha-naphtol
22
Q

What are the reagents used in a citrate test? (3)

A
  1. Sodium citrate
  2. Agar
  3. Bromothymol blue
23
Q

What are the reagents used in a KOH test? (1)

A

KOH

24
Q

What are the reagents used in an oxidase test? (1)

A

ADMA oxalate

25
Q

What are the reagents used in an ONPG test? (1)

A

ONPG

26
Q

What are the reagents used in an indole test? (3)

A
  1. Kovac’s reagent
  2. Nutrient broth containing no glucose and with added Trp
  3. DMAB
27
Q

What does MacConkey agar contain? (5)

A
  1. Bile salts
  2. Neutral red
  3. Crystal violet
  4. Lactose
  5. Peptone
28
Q

What does EMB agar contain? (4)

A
  1. Eosin
  2. Methylene blue
  3. Peptone
  4. Lactose
29
Q

What are the reagents used in a catalase test? (1)

A

H2O2

30
Q

What are the reagents used in a nitrate reduction test? (5)

A
  1. Nitrate broth
  2. alpha-naphylamine
  3. zinc powder
  4. sulfanillic acid
  5. Durham tube
31
Q

What does blood agar contain?

A
  1. Sheep’s blood
  2. NaCl
  3. Nutrient agar
32
Q

What does mannitol salt agar contain?

A
  1. Mannitol
  2. NaCl
  3. Peptone
  4. Phenol red

Introduction to Microbiology (18 decks)

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