Metabolic Testing Of Bacteria- Lab14-19 Flashcards Preview

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Flashcards in Metabolic Testing Of Bacteria- Lab14-19 Deck (25)
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1

What does amylase test test for

Amylase

2

What is the medium for the amylase test

Starch agar

3

What is the reagent for the amylase test

Iodine ; it turns the agar brown

4

Describe the amylase test

Two plates with straight streaks on them

5

Describe A positive result of the amylase test

Clearing around the growth line because the exoenzyme digested the starch

6

What does the lipase test test for

Lipase

7

What medium does a lipase test use

Tributyrin agar (cloudy vs. clear agar)

8

Describe a positive result for the lipase test

Clearing around the grounds line; obvious contrast because it ate up the fat

9

What does a gelatinase test test for

Gelatinase

10

What is the medium used for gelatinase test

Gelatin agar at 22°C (incubated at cooler temperature than the others)

11

Describe a positive result for a gelatinase test

Clearing around the growth line

12

Name the tests for enzymes of respiration

Catalase test , oxidase test , and nitrate reduction test

13

What does a catalase test test for

Catalase

14

What medium does a catalase test use

No medium- hydrogen peroxide(substrate)

15

Describe A positive result for the catalase test

Bubbles because of the oxygen gas

16

What does the oxidase test test for

Cytochrome C oxidase

17

What medium does the oxidase test use

BBL dry slide oxidase slide with reagent

18

What reagent does the oxidase test use

Reagent built into card

19

Describe a positive result for the oxidase test

Purple spot on slide in 20 seconds

20

What does the nitrate reduction test test for

Nitrate reductase

21

What medium was use in day one for the nitrate reduction test

Nitrate broth

22

What medium was used in day two of the nitrate reduction test

Nitrate test - hydrogen sulfide and TDR
nitrate test - zinc

23

Describe a positive test result for the nitrate reduction test

Bubble-nitrogen gas
Purple-Brown color

24

Name the catabolism of carbohydrates tests

Study the charts from lab 17 and 18

25

Name the tests for exoenzymes

Amylase test , lipase test , gelatinase test