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IS > MHC > Flashcards

MHC Flashcards

1
Q

HLA-B27

A

Ankylosing spondylitis

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2
Q

HLA-A29

A

Birdshot retinopathy

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3
Q

HLA-DR3, HLA-DR5, HLA-DR7

A

Celiac Disease

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4
Q

HLA-DR3

A

Graves’ Disease

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5
Q

HLA-DR2

A

Narcolepsy & Multiple Schlerosis

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6
Q

HLA-DR4

A

Rheumatoid Arthritis

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7
Q

HLA-DQ8, HLA-DQ2, HLA-DR3, HLA-DR4

A

Type 1 diabetes mellitus

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8
Q

Non Classical Class 1

A

HLA-E, HLA-F, HLA-G

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9
Q

Non Classical Class II

A

HLA-DM, HLA-DN, HLA-DO

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10
Q

Major antigen source of Endocytic Pathway

A

Extracellular & Membrane Proteins (host & foreign)

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11
Q

Processing Machinery for Endocytic Pathway

A

Lysosomal Enzymes

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12
Q

Cell Types where active

A

Professional APCs

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13
Q

MHC Binding site of antigen in Endocytic Pathway

A

Endocytic vesicles, prelysosomes

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14
Q

in Antigen pathway, the Endocytic Pathway is utilized in:

A

Class II

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15
Q

(Antigen Pathway) Endocytic Pathway is presents to:

A

CD4+ (helper) T Cells

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16
Q

Peptide Binding Site of Class I

A

a1 & a2

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17
Q

MHC CLASS I MOLECULES:

A

HLA-A, HLA-B,HLA-C

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18
Q

CLASS II MOLECULES:

A

HLA-DR, HLA-DQ, HLA-DP

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19
Q

Peptide Binding Site of Class II

A

a1 & b1

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20
Q

How many amino acids are needed in Class I?

A

8-10 amino acids

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21
Q

It is similar to the constants regions found in the immunoglobulin molecules.

A

a3 & B2 region

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22
Q

It reacts with CD8+ on cytotoxic T cells.

A

a3

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23
Q

Where do we found the Class II on antigen-presenting cells?

A

B cells, Macrophages, Monocytes,dendritic cells

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24
Q

molecular weight of alpha chain in Class II?

A

33kD

25
Q

Molecular weight of bets chain in Class II

A

27kD

26
Q

alleles in HLA-A, HLA-B, HLA-C?

A

HLA-A= 580 alleles
HLA-B= 921 alleles
HLA-C= 312 alleles

27
Q

It contacts sequences in the B2 domain

A

CD4+

28
Q

How many amino acid does Class II peptide needs?

A

9-20 amino acids

29
Q

Rabbit serum Incubation

A

RT: 60 mins

30
Q

battery of antisera known HLA Specificity

A

RT: 30 mins

31
Q

HLA Phenotyping

A

Serological techniques

32
Q

HLA Genotyping

A

Molecular Methods

33
Q

3 Histocompatibility testing

A
  1. Tissue testing
  2. Antibody Screening
  3. Tissue Matching/crossmatching
34
Q

Anticoagulated whole blood is overlaid to:

A

-Ficoll-Hypaque reagent
-then centrifuge

35
Q

Characterized antibodies within WBCs

A

Dausset

36
Q

Use of T Lymphocytes

A

Class I (HLA-A, -B, -C)

37
Q

It use B lymphocytes

A

Class II (HLA-DR, HLA-DQ, HLA-DP)

38
Q

3 B Lymphocytes separation:

A
  1. Nylon Wool separation
  2. Use of immunomagnetic beads
  3. Fluorescent labeling ( Use of FITC)
39
Q

FITC means:

A

Fluorescein Isothiocyanate

40
Q

B cells adhere, physically remove

A

Nylon wool separation

41
Q

It bounds to certain Antibody

A

Use of immunomagnetic beads

42
Q

It binds to immunoglobulin

A

Fluorescein Isothiocyanate/ fluorescent labeling

43
Q

Women with several pregnancy

A

Multiparous Women

44
Q

Exposed to several HLA

A

px who received multiple transfusions (Platelets&WBCs)

45
Q

what Dye is used in Complement-dependent lymphocytotoxicity

A

Trypan Blue dye (eosin Y)

46
Q

True/False:
Probability that any two individuals will express the same MHC molecule is very high.

A

False (very low)

47
Q

Interpretation of 0-10% Dead Lymphocyte

A

Negative

48
Q

Score & Interpretation of 11-20% dead Lymphocyte

A

2, Doubtful positive

49
Q

It has a score 4 and Weak positive interpretation

A

21-50%

50
Q

What is the score and % dead lymphocyte of the Positive interpretation?

A

6, 51-80%

51
Q

What is the interpretation of 81-100% Dead Lymphocytes?

A

Strong positive

52
Q

What PCR Based Technique is: PCR- amplification of a chosen sequence using primers flanking the sequence; The amplified DNA is immobilized in membrane

A

Sequence Specific Oligonucleotides

53
Q

Oligonucleotide primer are designed to obtain amplification of specific alleles of group or alleles

A

Sequence-Specific Primers

54
Q

The Sequence-Specific Primers are detected by:

A

Agarose Gel Electrophoresis & Transillumination

55
Q

2 Methods of Sequence Based Typing

A

-Sanger-based DNA sequencing
-Next generation DNA sequencing

56
Q

4 types of Grafts

A
  1. Autograft
  2. Syngraft
  3. Allograft (Homograft)
  4. Xenograft (Heterograft)
57
Q

What are the Tissues used in Transplantation?

A

-Kidney
-Heart
-Cornea
-Lung
-Skin
-Bone Marrow

58
Q

2 types of Graft-versus-Host Disease

A

-Acute GVHD
-Chronic GVHD

IS (4 decks)

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