Micro Flashcards

1
Q

Order and times of gram stain reagents

A
  1. Crystal Violet- 1min.
  2. Gram’s Iodine- 1min.
  3. Decolorizer- Until clear
  4. Safranin- 1min.
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2
Q

What color do Gram Positive bacteria stain? And why?

A

Purple

The thin lipid layer is effected by the decolorizer and the pink washes away

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3
Q

What color do Gram Negative bacteria stain?

A

Pink

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4
Q

What 2 species are very susceptible to Tetanus and should be immunized

A

Humans & Horses

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5
Q

The Coggin’s Test is used to diagnose ___

A

EIA (Equine Infectious Anemia)

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6
Q

What is used for a Catalase Test?

A

Hydrogen Peroxide

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7
Q

A positive Catalase Test has what reaction? Meaning it is _____

A

Bubbles Staphylococcus

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8
Q

A negative Catalase Test has what reaction? Meaning it is _____

A

None Streptococcus

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9
Q

A positive Coagulase test will have what reaction? Meaning it is ____

A

Plasma will clot Pathogenic Staphylococcus

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10
Q

A Coagulase test is done on which bacteria?

A

Gram pos. cocci that have tested positive on the Catalase test

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11
Q

A negative Coagulase test will have what reaction? Meaning it is ____

A

No change Nonpathogenic Staphylococcus

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12
Q

What is Beta hemolysis? What bacteria are indicated by this reaction?

A

Complete lysis of RBC’s in blood agar Pathogenic Streptococcus

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13
Q

What is Alpha hemolysis? What 4 types of bacteria are indicated by this reaction?

A

Incomplete lysis of RBC’s in blood agar Strep.Pneumoniae Strep.Suis Strep.Bovis Strep.Equis

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14
Q

What is Gamma hemolysis?

A

No lysis of RBC’s in blood agar

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15
Q

MaConkeys agar selects for the growth of ____ and differentiates between ____ and ____

A

GNRODs Lactose and Non-Lactose fermenters Lactose= bright pink Non-Lactose= colorless

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16
Q

What is FIV and what causes it?

A

Feline Immunodeficiency Virus

Retroviridae- Lentevirus

Biting is the most common means of transmission it is frequently seen in aggressive, free-roaming, male cats

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17
Q

Pregnant women should avoid cleaning litter boxes due to ____

A

Toxoplasmosis gondi

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18
Q

Common name for the yeast that causes otitis in dogs?

A

Malassezia

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19
Q

What is equine strangles caused by?

A

Streptococcus equi

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20
Q

What causes circling disease?

A

Listeria monocytogenes

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21
Q

What causes Lyme disease?

A

a Spirochete-Borrelia burgdorferi Transmitted by tick

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22
Q

What causes Cat Scratch Disease?

A

Bartonella henselae

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23
Q

What causes Rocky Mountain Spotted fever?

A

Rickettsia ricketsii

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24
Q

What are the 2 types of Gram Pos. bacteria?

A

Staphylococcus Streptococcus

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25
What are the 5 small Gram Pos. rods?
Corneybacterium Listeria Erysipelothrix Arcanobacterium Rhodococcus (more important in large animals)
26
What are the 2 large Gram Pos. rods?
Bacillus Clostridium
27
What are the 4 branching Gram Pos. rods?
Actinomyces Nocardia Dermatophilus Streptomyces
28
What is the Kirby-Bauer susceptibility test?
Test used to test certain bacteria's resistance to certain types of antibiotics Area around disc is measured in mm Resistant= no clearing Intermediate= some clearing may or may not work Susceptible= clearing around disc- drug should work
29
What are 4 common bacteria known to cause mastitis?
Staph. aureus Staph. Agalactica E. cli Mycoplasma bovis
30
How is Malassezia diagnosed?
Ear swab Roll on slide Heat fix Stain with Wright's Look for peanut men
31
A. GNRODs B. GPRODs C.GNCocci D.GPCocci
GPRODs
32
A. GNRODs B. GPRODs C.GNCocci D.GPCocci
GNRODs
33
A. GNRODs B. GPRODs C.GNCocci D.GPCocci
GPCocci
34
A. GNRODs B. GPRODs C.GNCocci D.GPCocci
GPCocci
35
ID
Giardia Trophozyte
36
ID
Otodectes (Ear Mite)
37
ID
Microsporum Canis | (Ringworm)
38
ID
Clostridium Perfringes
39
ID
Campylobacter
40
ID
Coccidia Isospora
41
ID
Malassezia (Yeast)
42
Rhodococcus Equi
Bronchopneumonia in foals Small Gram pos. Rods Abscess in lungs
43
Bacillus Anthracis
Anthrax Large Gram + Rod Acquired by inhaling spores
44
Clostridium Perfringes
Clostridial enterotoxemia Large Gram + Rods Safety pins
45
Yersinia pestis
Gram - Rod Causes the plague Host= Rodents (prarie dogs)
46
Bordetella bronchiseptica
Kennel Cough non-enteric Gram - Rod
47
Campylobacter
Gram - curved Rods Usually chain linked Scours in cattle Wet tail in hamsters
48
Mycobacterium
Tuberculosis
49
Chlamydophila psittaci
Pisttacosis "Parrot fever"
50
Actinomyces Bovis
Lumpy Jaw Branching Gram + Rod Abscesses around head/neck region
51
Clostridium Haemolyticum
Red Water Disease Large Gram + Rod Dark red urine
52
Dermatophilus Congolensis
Rain Rot Branching Gram + Rod Likes humidity Localized dermatitis Lesions & scabs
53
Streptococcus Equi
Equine Strangles Gram + Cocci Beta hemolysis Enlarged lymphnodes
54
Streptococcus Agalactae
Bovine Mastitis Gram + Cocci Beta
55
Staph Aureus
MRSA Gram + cocci
56
MRSA
Methicillin Resistant Staph Aureus
57
Corynebacterium pseduotuberculosis
Pigeon Fever Small Gram + Rods Vector= flies
58
Listeria monocytogenes
Circling dz. Small Gram + Rod Acquired from contaminated food
59
Clostridium botulinum
Limberneck in birds Shaker foal in horses Large Gram + Rod
60
Clostridium tetani
Tetanus/ Lock jaw Large Gram + Rod Humans and horses enther through puncture wound
61
Clostridium Chauvoei
Black Leg Large Gram + Rod Liver necrosis
62
Staph Hyicus
Greasy pig disease Gram + Cocci
63
Bartonella henselae
Cat Scratch Disease Vector= fleas
64
Rickettsia rickettsii
Rocky Mountain Spotted Fever Vector= tick
65
What is the #1 cause of ringworm in dogs & cats? How do you diagnose?
Microsporum Canis Woods lamp
66
Rhabdo viridae
Rabies
67
Retro viridae
FeLV
68
Malassezia pachydermatis
Otitis Dermatitis
69
Mycoplasma haemofelis
Feline Infectious Anemia Vector= Ticks/Fleas Lives on RBCs
70
EEE
Eastern Equine Encephalomyelitis
71
E.coli is a Gram ____ Rod that commonly causes \_\_\_\_.
Negative Cystitis
72
Common name for Rhipicephalus sanguineus?
Brown Dog tick
73
How does yeast reproduce?
By budding