Micro

Question 1

Control agent in Hot Air Oven? .

Answer 1

B- SubtiliS 1 Atrophies/ Clost tetani
160×2hr
170× 1 hr
180× 30 min
glass wares, cotton swabs, liq. paraffin wax, dusting powder

Question 2

Holder’s method of pasteurisation?

Answer 2

63°C×30 mins
Flash method-72 ° C x 20 sec kills Coxiella burnetti
MoistHeat method

Question 3

LJ media sterelised by

Answer 3

Inspissation

Question 4

Inspissation

Answer 4

80_85 ° C x 20-30mins x 3 days

LJ media, LSS - Coryn diphtheriae

Question 5

Tyndallisation

Answer 5

loo°Cx 20min x 3 days kill spores
Normal Boiling-15 mins- will not kill spores

Question 6

Autoclave?

Answer 6

Moist method temp > 100C

121 °C x 15min x 15 psi
for prions- 134°C x 1-5 hours

Question 7

Bio control agent in Autoclave

Answer 7

Bacillus stearothermophilas

Question 8

Autoclave used for

Answer 8

Instruments, Aprons
Sutures except catgut
Media except LJ & LSS
Sputum

Question 9

Ionising Radiation Includes

Answer 9

X-Rays and gamma
Cold Sterilisation_no Increase in temp
used for catgut sutures , pre packed syringes, grafts

Question 10

Control agent for gas sterilisation? ETO

Answer 10

B. globogi

Question 11

Cidex

Answer 11

2.4 %. glutaraldehyde

Question 12

Control agent for Radiation?

Answer 12

B. purmilis

Question 13

Blood spill in OT Cleaned with

Answer 13

1% hypochlorite

Question 14

Fried egg Appearance

Answer 14

Mycoplasma
Malassezia furfur
oligo- dendroglioma
Hairy cell leukemia
Seminoma/ Dysgeominone

Question 15

Malassezia furfur

Answer 15

Hypopigmented lesions over back & trunk
KoHmount - Banana $ grapes App, Sphagetti & meatball
culture. Fried egg App.

Question 16

Tinea versicolor

Answer 16

M. furfur

Question 17

On LPCB, Broom, Brush App.

Answer 17

penicillium marneffi= talaromyces marneffi
natural Reservoir- Bamboo Rats

Question 18

South American Blastomycosis

Answer 18

paracoccidiomycosis = P. brasiliensis
Mickey Mouse App on M/E pilot wheel App.

Question 19

CocCidiomycosis

Answer 19

Dessest Rheumatism/ Valley fever

Question 20

Barrel shaped Arthrospores
M/ E - Spherule with endosposes

Answer 20

Coccidiomycosis - Dessert Rheumatism

Question 21

Rose gardener’s disease

Answer 21

Sporotrichosis - Thorn prick
Sporothrix Schinekii
m/E - Cigar body, Asteroidbody
Culture- Rossette of conidia

Question 22

Asteroid body seen in

Answer 22

Sporotrichosis, Sarcoidosis
Sarcoidosis - Intracellular Asteroid body non_ cesseating granuloma, giant cells, Star seen in giant cell

Question 23

pt coming with multiple discharging sinus. D/d?

Answer 23

Eumycetome - Madurela mycetOmatis- grains contain fungal hyphae (> 2µ)
Actinomycetes- G + filamentous bacteria (<2µ) AC, nomedura medurae, Actinomyces, Nocardia

Question 24

ping pong ball appearance

Answer 24

pneumocystistis jirovici/ carini
opportunistic Infection
plasma cell pneumonia
Crushed ping pong /ping pong ball App.
Hat App
Cup & Saucer App
Cannot be Cultivated

Question 25

Aspergillus- diff species

Answer 25

1. Aspergillus niger- Black 2. Aspergillus fumigatus- smoky green 3. Aspergillus flavus- yellow Aspergillus - Acute Angle branching septate hyphae

Question 26

Which sp. of Candida show germ Tube growth

Answer 26

C Albicans and C Dubliniensis They both show Chlamydospore formn as well

Question 27

West African sleeping sickness?

Answer 27

Trypanosoma brucei gambiense tse tse fly glossina palpalis

Question 28

East African sleeping Sickness?

Answer 28

T.b. rhodesiense Tse Tse fly glossing morsitans

Question 29

chagas dis

Answer 29

Trypanosoma cruzi

Question 30

leishmania donovani nfective form?

Answer 30

Promastigote diagnostic F= Asmastigote

Question 31

Triad of Congenital CMV?

Answer 31

Chorio-retinitis Hydro cephalus Intra-cranial calcification

Question 32

Def & lnt Host Toxoplasma

Answer 32

Def= Cat Int= Human

Question 33

Chinese liver Fluke

Answer 33

Clonorchis sinensis

Question 34

South east Ascian L fluke

Answer 34

Opisthorchis viverrini

Question 35

Cat liver Fluke

Answer 35

Opisthorchis felineus

Question 36

Sheep liver FIuke

Answer 36

Fasciola hepatica

Question 37

giant Iiver Fluke

Answer 37

Fasciola gigantica

Question 38

Operculated eggs seen in

Answer 38

Spirometra All trematodes except schisto Soma DiphyllobOthriUm latum