Micro Flashcards

1
Q

Purpose of peptidoglycans

A

gives rigid support, protects against osmotic pressure, determines how much gram strain will be picked up. Sugar backbone with peptide side chain cross linked by transpeptidase

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2
Q

Gram + vs gram - color

A

Gram + pruple, gram - pink

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3
Q

function of cell wall/cell membrane (gram +)

A

major surface antigen, induces TNF and IL1

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4
Q

function of outer membrane (gram -)

A

site of endotoxin, lipid A induces TNF and IL-1

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5
Q

periplasm function

A

space btwn cytoplasmic membrane and outer membrane in gram negative bactr, contains hydrolytic enzymes including B lactamases

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6
Q

glycocalyx function

A

mediates adherence to surfaces, especially foreign substances – sugar coat, think biofilm/capsule

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7
Q

Gram + bacteria examples

A
  • Staph, strep (cocci)
  • clostridium, corynebacterium, bacillus, listeria, mycobacterium,gardnerella (bacillus)
  • actinomyces, nocardia (branching)
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8
Q

Gram negative examples

A

-neisseria (coccus)

==RODS==

  • Ecoli, shigella, salmonella, yersinia, klebsiella, proteus,enterobacter,serratia, bivrio, campylobacter,helicobacte,r psuedomonas, bacteriodes(enterics)
  • haemophilus, legionella, bordetella (respiratory)
  • francisella, brucella, pasteurella, bartonella (zoonotic)
  • leptospira, borrelia, treponema (spirochete)
  • ricketssiea,chlamydia (plenomorphic)
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9
Q

Unique thing about cell membrane/wall for mycoplasma

A

contains sterols and has no cell wall (hence why some abx dont work)

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10
Q

Unique thing about mycobacteria cell wall

A

contain mycolic acid,high lipid content

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11
Q

Capsule function in bacteria

A

protects against phagocytosis,usually polysaccharide (except D-glutamate in bacillus anthracis)

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12
Q

Bugs which dont gram stain well

A

“These Rascals May Microscopically Lack Color”
-Treponema, Rickettsia, Mycobacteria, Mycoplasma, Legionella, Chlaymdia

can use silver stain for legionella

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13
Q

for waht bugs do you use Giemsa stains

A

Chlamydia, Borrelia,Rickettsiae, Trypanosomes, Plasmodium,Histoplasm

“Certian Bugs Really Try His Patience”

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14
Q

PAS stains for..

A

Stains glycogen/mucopollysacharides –> Tropheryma whipplei

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15
Q

Ziehl Neelson stain

A

Acid Fast organisms (nocardia, mycobacteria)

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16
Q

India Ink stain

A

cryptococcus neoformans

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17
Q

Bug grown on Chocolate agar w Factors V (NAD+) and X (hematin)

A

H. Influenza

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18
Q

Culture reqs for H Infleunza

A

Chocolate Agar w/ FV and FX (NAD+ and hematin, respectively)

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19
Q

Thayer martin media grows what two bugs, and has what 3 bugs

A

Neissseria gonorrhoea and neisoerria meningitidis

VPN: Vancomycin, polymyxin, nystatin

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20
Q

Bordet-Gengou (potato) agar grows

A

Bordetella

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21
Q

Loffler’s Media grows

A

C Diphtheriae

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22
Q

Lowenstein Jensen agar grows

A

TB

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23
Q

Eatons agar grows

A

M pneumoniae

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24
Q

MacConkey’s agar grows, and what color do fermenting bac grow

A

they turn pink; Think lactose fermenting bacteria (Citrobacter, Ecoli,klebsiella, enterobacter, serratia)

CKEES

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25
Q

Charcoal yeast extract grows

A

Legionella

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26
Q

Sabarouds agar grows

A

Fungi

“Sabaroud is a fun guy”

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27
Q

Obligate aerobes include

A

Are O2 depedent, and includes

Nocardia, pseudomonas, mycobacterum tberculosis, bacillus

“Nagging Pests Must Breathe”

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28
Q

Obligate anaerobes include

typical abx include?

A

Clostridium, bacteriodes, actinomyces

they lack catalose/superoxide dismutase –> are susceptible to oxidative damage, genreally foul smeling
Tx: clindamycin, metronidazole

“can’t breathe air”

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29
Q

Obligate intracellular bugs

A

Rickettsia and Chlamydia (cant make their own ATP)

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30
Q

Facultative Intracellular

A

Salmonella, Neisseria, Brucella, Mycobacterium, Listeria,Francisella, Legionella, Yersinia estis

“Some Nasty Bugs May Live FacultativeLY”

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31
Q

Encapsulated Bacteria have what + reaction, and include what species?

A

+ quellung reaction

Strep pneumo, Haemophils influenza, Neisseria Meningitids, E Coli, Salmonella, Klebsiella, GBS, Pseudomonas

“Even Some Pretty Nasty Killers Have Shiny Bodies”

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32
Q

Quellung reaction indicates what?

A

Encapsulated species

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33
Q

Catalase + organisms include

Also, pts with what disease are more prone to these infections?

A

Pseudomonas, Listeria, Aspergillus, Candidda, EColi, Staph Aureus, Serratia

Pts with chronic granulomatous disease have recurrent infxns

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34
Q

Pigment of the following bacteria:

  • Staph Aureus
  • Actinomycess Israelii
  • Serratia Marcescens
  • Pseudomonas Aeurginosa
A
  • gold
  • gold
  • red
  • green
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35
Q

Importance of bacterial viurlence factor Protein A, which bug has it?

A

Binds Fc region of Ig, prevents opsonization and phagocytosis – staph aureus

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36
Q

Importance of bacteriaul virulence factor - IgA protease, who has it?

A

Enzyme cleaves IgA, Strep Pneumo, H Influenza, Neisseria (SHiN)

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37
Q

Importance of bacterial virulence factor M protein - who has it

A

prevents phagocytosis, seen on Group A strep

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38
Q

More toxic: endo or exotoxin?

A

Exotoxin

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39
Q

Result of Endotoxin

A
  • Activates macrophages (IL1, TNF, and NO release)
  • Activates complement (C3a, C5a)
  • Activates tissue factor (DIC)

ENDOTOXIN:
Edema, NO, DIC/Death, Outer Membrane, TNFa, O-antigen, eXtremely heat stable, IL-1, Neutrophil chemotaxis

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40
Q

Toxin associated with corynebacterium diptheria and MOA

A

Diptheria toxin, inactivates elongation factor 2

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41
Q

Pseudomonas aeruginosa toxin and MOA

A

Exotoxin A - inacitvates elongation factor 2

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42
Q

Shigella toxin and MOA

A

shiga toxin - inactivates 60S ribosome by removing adenine from rRNA - causes GI mucosal damage –> dysentery

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43
Q

EHEC toxin and moa

A

Shiga Like Toxin (verocytotoxin) which inactivates 60S ribosome by removing adenine from rRNA

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44
Q

two toxins of ETEC and MOA

A

increased fluid secretion

LT and ST (heat labile and heat stable)

  • LT activates adenylate cyclase, leading to increased cAMP –> increase Cl secretion in gut and H2o efflux)
  • ST activates guanylyl cyclase,leading to increased cGAMP –> decreased NaCl and H2o resoption in gut

Wattery diarrhea: labile in the Air (cAMP) and stabile on the ground (cGMP)

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45
Q

Bacillus anthracis 3 toxins and MOA

A

edema factor, lethal factor, protective Ag

mimics adeylate cylcase activity (increased cAMP), causes increased fluid secretion

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46
Q

vibrio cholera releases..

A

cholera toxin, overactivates adenylate cycalse (cAMP) by permanently activating Gs –> incnreased CL- and H2O secretion in gut

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47
Q

Bodetella pertussis toxin and activity

What disease is caused?

A

Pertussis toxin which activates adenylate cycalse –> increased cAMP, impairs phagytosis
causes whooping cough

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48
Q

Clotridium tetanus rleases what toxin, and what MOA?

A

Tetanospasmin, cleaves SNARE protein required for NTM release, prevents release of inhibitory (GABA and glycine) NTM,causing muscle rigidity

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49
Q

Clostriduum botuliniuum has what exotoxins

A

Botulinum toxin, cleaves SNARE protein required for NTM release, prevents release of stimulatory (ACh) signal at NMJ, causes flaccid paralysis

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50
Q

Yersinia Enteridis toxin, associated with what disease?

A

similar to ETEC- overactivates cGMP, causes abdpominal pain, “psuedo appendicitis”

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51
Q

Clostridium perfringens releases what two toxins and has what MOA

A

releases alpha toxin, which is a phospholipase which degrades tissue and cell membranes.

also has enterotoxin for food poisoning

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52
Q

Strep pyogenes exotoxin

A

Streptolysin O, which degades cell membranes

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53
Q

Staph Aureus Toxins (x2?)

A

TSST-1 and Enterotoxins

TSST brings MHC II and TCR in proximity, causing overwhelming release of IFNy and IL-2 –> shock

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54
Q

Strep pyogenes (GAS) has what superantigen and what MOA?

A

Exotoxin A –> brings MHC II and TCR in proximity, causing overwhelming release of IFNy and IL-2 –> shock

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55
Q

Meaning of alpha vs beta vs gamma hemolysis

A

Alpha - partial hemolysis with green cleaning (strep pneumo or strep viridans)

Beta - completel hemolysis with white clearing (GAS) - Strep pyogenes or GBS (Strep agalictiae)

Gamma - no hemolysis, no clearining - Enterococcus

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56
Q

Clinical presentation of Strep Pneumo

A

Meningitis, Otitis Media, Pneumonia, Sinusitis

MOPS

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57
Q

Staph exotoxins can cause what?

A

Shock, Staph Scalded skin Syndrome (TSST)

gastroenteritis (enterotoxin)

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58
Q

Rheumatic Fever Criteria?

A

JONES

  • Joint pains
  • Pancarditis
  • Nodules
  • Erythema marginatum
  • Sydenham’s Chorea
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59
Q

Toxins associated with Strep Pyogenes (GAS)

A
  • Streptlysin O –> hemolysis, O2 labile
  • Streptolysin S - hemolysis, O2 stable
  • Eryhtogenic/Pyogenic toxins –> Skin rash, fever, scarlet fever
  • Exotoxin : toxic shock like syndrome
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60
Q

Pyogenic, Toxigenic, and Immunologic result of GAS/Strep Pyogenes

A
  1. pharyngitis, cellulitis, impetigo
  2. Scarletfever, toxic shock like syndrome, necrotizing fasciitis
  3. PSGN, Rheumatic Fever
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61
Q

causes of cellulitis/impetigo

A

Staph aureus and strep pyogenes

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62
Q

Clostridium difficile’s two toxins MOA

Often secondary to what abx use?

A

Toxin A: enterotoxin binds to brush border of gut
Toxin B: cyotoxin, destroys the cytoskeletal structure of enterocytes, causing psuedomembranous colitis

Usually clindamycin, ampicillin

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63
Q

Waterhouse friderichsen syndrome? caused by?

A

Adrenal hemorrhage, casued by N. Meningococcus

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64
Q

Hemophiluscuases what sx?

A

hEMOPhilus

Epiglottis, meningitis, otitis media/sinusitis, pneumonia

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65
Q

Psuedomonas Aeurgionsa causes what disease?

A

PSUEDOmonas
Pnuemonia, Sepsis, Externial otitis media, UTI, Drug use, and diabetic osteomyleitis

Produces endotoxin and exotoxin A

66
Q

Kelbsiella’s 4 A’s

A

Aspiration pnuemonia, abscess in lungs and liver, Alcoholic, diAbetics

67
Q

Lyme dz manifestations:

A

FAKE:

Facial nerve palsy,arhtralgias, kardiacblock, erythyma migrans

68
Q

associated with cat scratch disease

A

bartonella spp

69
Q

cat scratch in immunocompromised patient

A

bacillary angiomatosis, regional LAD

70
Q

Borrelia burgdoferia

A

lyme dz, caused by ixodes tick

71
Q

unpasteurized dairy

A

brucella, brucellosis

72
Q

puppies/live strock (ingestion of undercooked meat)

A

campylobacter, bloody diarrhea

73
Q

parrots, other birds

A

chlamydiophila psittaci, psittacocis, severe PNA

74
Q

Aerosols of cattle, sheep amniotic fluid

A

Q fever, coxiella burnetii

75
Q

Lone star tick

A

Ehrlichiosis, ehrlichia chaffeensis

76
Q

Animal urine transmission

A

leptospira,leptospirosis

77
Q

Armadillos and humans with specific skin dzL

A

mycobacterium leprae, leprosy

78
Q

animal bite, cats,dogs

A

pasteurerlla multicoccida, causes cellulitis and osteomyelitis

79
Q

louse

A

rickettsia (epidemic typhus) or borrelia

80
Q

dermacentor tick bite

A

Rocky mountain potted fever, rickettsia rickettsii

81
Q

Fleas

A

Endemic typgus, rickettsia typhi

82
Q

Fleas, rats, and prairie dogs

A

Yersinia pestis, bubonic plaque

83
Q

Rickettsial diseases

  • Triad
  • can all be treated with
  • Weil-felix rxn + or -?
A
  • HA, fever, Rash
  • Doxycycline
    • weill felix rxn bc of Ab agglutination
84
Q

Palm and Sole rash seen with which infections?

A

Coxsackie A, RMSF, secondary syphilus (CARS)

85
Q

5 organisms assocaited with birds

A

Histoplasma, cryptococcus neoformans, chlamydophyla psittaci,WNV, H5N1

86
Q

Disease location of:

  • Histoplasmosis
  • Blastomycosis
  • Coccidiomycosis
  • Paracoccidiomycosis
A
  • histo: Mississippi and Ohio River valleys (found with bird/bat droppings)
  • blasto: East of mississippi river, central america
  • SW US, California
  • Latin Aerica - looks like captains wheel
87
Q

4 main classes of infections

A

Bacteria, VIruses, Fungi, Parasites

88
Q

Protozoa causing

-GI, CNS, Heme, Visceral, STDs infxns

A

GI: Giardia, entomoeba histolytica, cryptospordium

CNS: toxoplasma gondii, naegleria fowelderia, Trypanosoma

Heme: plasmodium, Babesia

Viscerial: Leschmaniasis, trypanosmaCruzii

STD: trichomonas

89
Q

3 main types of parasites cause disease in humans:

A
  • Protozoa - usually unicellular)
  • Helminths - multicellular (3 main groups are nematodes (roundworms), cestodes (tapeworms), and trematodes (flukes)
  • Ectoparasites (think ticks, fleas, mites, and lice)
90
Q

Nematode route of infection

A

Ingested: enterobius, Ascaris, Trichinella
Cutaneous: Strongyloides, Ancylostoma, Necotar

You’ll get sick if you EAT these!
These get into your feet from the SANd!

91
Q

Brain cysts, seizures

A

Taneia solium (cysticerciosis)

92
Q

Liver cysts

A

Echinoccocus granulosus

93
Q

VitB12 Deficiency

A

Diphyllobothrium

94
Q

Biliary tract disease,cholangiocarcinoma

A

Clonorchis sinensis

95
Q

Hemoptysis

A

Paragonismus westermani

96
Q

Portal HTN

A

Schistosoma mansoni

97
Q

Hematuria, bladder cancer

A

Schistostoma haemtobium

98
Q

Microcytic anemia

A

Ancylostoma, necator

99
Q

Perianal pruiritis

A

Enterobius

100
Q

Live viruses:

A

Smallpox,yellow fever, VZV, Sabin’s polio, MMR, and nasal infleunza,rotavirus

MS. PRIVY

101
Q

Killed Virus

A

Rabies, Influenza, Salk Polio, HAV vaccines

PAIR

102
Q

Vaccines with egg

A

Yellow fever, Influenza

103
Q

All RNA viruses except _____ are ssRNA

A

reovirus and retrovirus

104
Q

Positive stranded RNA viruses

A

retrovirus, togavirus, flavivurus, coronavirus,hepevirus, calicivurus, picornavirus

105
Q

All viruses are ____loid except for ___ which are ____

A

all viruses are haploid, except retrovruses, which are diploid (have 2 ssRNA)

106
Q

All DNA viruses replicate in ____ except ____

A

nucleus,poxvirus (smallpox, molluscum contagiosum)

107
Q

All RNA viruses replicate in ____ except ___

A

cytoplasm, influenza/retroviruses

108
Q

DNA viruses include

A

Hepadna, herpes, adeno, pox, parvo,papiloma, and polyoma virus

109
Q

Naked/noneveloped viruses include:

A

papillomavirus,adenovrus, picornavirus, polyomavirus,calcivurs, parvovirus,reovirus,and hepevirus

give PAPP (DNA) smears and CPR to a naked Heppy (RNA)

110
Q

Herpesvirus

Enveloped, DNA structure, and important viruses in family

A
HSV 1 - oral
HSV 2 - genital ulcers
VZV - 3
EBV  -4 
CMV - 5
HHV6 - roseola 
HHV7 - less common cause of roseola
HHV8 - Kaposi's sarcoma

yes envelope, DS linear
causes latent dz, reacitvated later

111
Q

Hepadnavirus

Enveloped, DNA structure, and important viruses in family

A

yes enveloped, DS and partial circular DNA

HepB Virus

112
Q

Adenovirus

Enveloped, DNA structure, and important viruses in family

A

No envelope, DS and linear DNA

febrile pharyngitis,PNA, conjunctivitis

113
Q

parvovirus

Enveloped, DNA structure, and important viruses in family

A

no envelope, SS and linear (negative strand)

parvo B19

114
Q

ppillomavirus

Enveloped, DNA structure, and important viruses in family

A

No envlope, DS and circular

HPV 1,2,6,11,16,18

115
Q

polyomavirus

Enveloped, DNA structure, and important viruses in family

A

No envelope, DS and circular

JC virus

116
Q

poxvirus

Enveloped, DNA structure, and important viruses in family

A

yes envelope, DS and linear

smallpox, vaccinia,and molluscum

117
Q

Reoviruses

Enveloped, RNA structure, and important viruses in family

A

no, DS linear

Coltivirus, Rotavirus

118
Q

1 cause of fatal diarrhea in children

A

Rotavirus

119
Q

Picornaviruses

Enveloped, RNA structure, and important viruses in family

A

no envelope, ss + linear, PERCH viruses

Poliovirus, Echovirus, Rhinovirus, Coxsackie viruses, HAV

120
Q

Hepevirus

Enveloped, RNA structure, and important viruses in family

A

no envelope, SS + linear, HEV

121
Q

Caliciviruses

Enveloped, RNA structure, and important viruses in family

A
No envelope, +SS linear
Norwalk viruse (think cruise ship diarrhea)
122
Q

Flavivirus

Enveloped, RNA structure, and important viruses in family

A

Yes envelope, SS + linear

HCV, yellow fever, dengue, WNV

123
Q

togavirus

Enveloped, RNA structure, and important viruses in family

A

yes enveloped, SS + linear

rubellea

124
Q

Retroviruses

Enveloped, RNA structure, and important viruses in family

A

yes enveloped, SS + inear

HIV, HTLV

125
Q

Coronaviruses

Enveloped, RNA structure, and important viruses in family

A

Yes enveloped, SS + linear,

Coronavirus – common cold

126
Q

Orthomyxovirus

Enveloped, RNA structure, and important viruses in family

A

yes enveloped, SS negative linear RNA

Infleunza virus

127
Q

Paramyxoviruses

Enveloped, RNA structure, and important viruses in family

A

Yes enveloped, SS negative linear RNA

parainfluenza (croup)
RSV (bronchiolitis)
measles, Mumps

128
Q

Rhabdoviruses

A

Rabies

129
Q

Filoviruses

Enveloped, RNA structure, and important viruses in family

A

yes enveloped, ss neg linear RNA

EBOLA, marburg hemorrhagic fever

130
Q

Negative stranded viruses

A

must tx negative stand to positive strand,virus brings its own RNA dependent RNA polymerase

Arenaviruses, Bunyaviruses,Paramyxoviruses, orthomyxoviruses, filoviruses, rhabdoviruses

Always Bring Polymerase Or Fail Replication

131
Q

Segmented viruses – DNA or RNA?

A

all are RNA

BOAR - Bunyaviruses, Orthomyxociurses, Arenaviruses,Reoviruses

132
Q

Cough, Coryza, COnjunctivitis?

A

MEASLES virus, a paramyxovirus, can see koplik spots

133
Q

parotitis, orchitis,meningitis

A

Mumps virus

134
Q

What can cause viral meningitis

A

Coxsackie virus,echovirus, enterovirus, mumps virus

135
Q

Hepataitis Viruses - what kind of family?

A
HAV = RNA picornavirus
HBV = DNA hepadnavirus
HCV = RNA flavivirus
HDV = RNA deltavirus
HEV =  RNA hepevirus
136
Q

Gp120 fxn?

gp41 fxn?

A
gp120  = attachment to host CD4+ T cell
gp41 = fusion and entry
137
Q

HIV p24 fxn?

A

Capsid protein around RNA

138
Q

3 genes of HIV

A

gag (p24),pol (reverse transcriptase,aspartate protease,integrase), env (gp120, gp41)

139
Q

Food poisoning from contaminated seafood

A

vibrio parahemolyticus/vulnificus

140
Q

food posinoining from reheated rice

A

bacillus cereus

141
Q

poisnoing from meats, mayo, custard

A

Staph aureus (PREFORMED TOXIN)

142
Q

reheated meat dishes

A

clostridium perfringens

143
Q

Clostridium botulinum

A

improperly canned foods

144
Q

undercooked meat

A

EColi (O157:H7)

145
Q

poultry, meat, eggs

A

Salmonella (no preformed toxin)

146
Q

Bloody diarrhea:

A

campylobacter,salmonella, shigella, EHEC, EIEC, Yersinia entercolatia, entomobea

147
Q

Watery diarrhea

A

ETEC, vibrio cholera, Cdiff, Cperfrigens, protozoa, viruses

148
Q

PNA nosocomial

A

Staph,enteric gram neg rods

149
Q

PNA in immuncomoromised

A

Staph, enteric gram neg rods, fungi, viruses, PCP

150
Q

aspiration PNA

A

Anaerobes (tx with clinda)

151
Q

alcoholic/IVDU PNA

A

klebsiella, staph, strep pneumo

152
Q

CF PNA

A

Pseudomonas, staph, strep

153
Q

Post viral PNA species?

A

Staph, HFlu, Strep pneumo

154
Q

Atpical PNA?

A

Mycoplasma, Legionealla, Chlamydia (tx with macrolides)

155
Q

COPD PNA?

A

HInfluenza

156
Q

most common cause of osteomyelitis

A

Staph aureus

157
Q

4 causes of meningitis in HIV?

A

Cryptococcus, CMV< toxoplasmosis, JV virus

158
Q

TORCH infections? their significance?

A

Toxoplasma,HSV2, Syphillis, Rubella, CMV, HIV – they can cause congenital malformations

159
Q

Chorioretinitis,hydrocephalus, and intracranial calficications?

A

Toxoplasma godi TORCH infxn

160
Q

PDA or pulm artery hyoplasia, cataracts, and deafness?

A

Rubella TORCH infxn

161
Q

hearing loss, seizures, petchieal rash

A

CMV TORCH infxn