Micro lab quiz 1

Question 1

Compound microscope uses_____sets of lenses

Answer 1

2

Question 2

first compound microscope was made ______ and it magnified______

Answer 2

1600…..3x9 times

Question 2

first man made magnifying lens dates back to…

Answer 2

1267

Question 3

scan lense magnification
lens:
mag of ocular:
total mag:

Answer 3

lens: 4x
mag of ocular: 10x
total mag: 40x

Question 3

high power lens
made in:
magnifies:

Answer 3

made in: 1830s
magnifies: 400x

Question 3

the ocular lens magnifies____

Answer 3

x10

Question 3

compound microscope uses _________ sets of lenses

Answer 3

2

Question 3

Oil immersion lens
made in:
magnifies:

Answer 3

made in: 1860s
magnifies: 1000x
allowing us to study bacterial morphology

Question 4

types of objective lenses

Answer 4

(closest to the specimen)
scan
low power
high (dry) power
oil immersions

Question 4

low power lense magnification
lens:
mag of ocular:
total mag:

Answer 4

lens: 10x
mag of ocular: 10x
total mag: 100x

Question 5

high (dry) power lense magnification
lens:
mag of ocular:
total mag:

Answer 5

lens: 40x
mag of ocular: 10x
total mag: 400x

Question 6

oil immersions lense magnification
lens:
mag of ocular:
total mag:

Answer 6

lens: 100x
mag of ocular: 10x
total mag: 1000x

Question 7

working distance definition

Answer 7

the distance between the objective lens and the specimen being observed is called working distance

Question 8

As magnification increases working distance decreases
true/false

check this

Answer 8

true

Question 9

As magnification increases working distance increases
true/false

Answer 9

true

Question 10

What is field of vision?

Answer 10

the area or portion of the specimen that is seen through the microscope (virtual image)

Question 11

as magnification increases field of vision decreases
true/false

Answer 11

true

Question 12

adjust diaphragm everytime you _________ magnification

Answer 12

increase

Question 13

a specimen is placed on a…

Answer 13

slide

Question 14

function of condenser lens?

Answer 14

• to bend light rays so they enter the objective lenses
• if condenser lens is not used , the light rays bend at such great angles they would not enter the objective lens
• not used in oil immersion lense

Question 15

Where is the IRIS DIAPHRAGM located ?

Answer 15

-betweeen the condenser and the light sources
- move lever to right to decrease light
- move to left to increase lighting

Question 16

when do you adjust the iris diaphragm to increase amount of light ?

Answer 16

• when changing objective lense for higher magnification

Question 17

(t/f) the size of the opening of the objective lenses decreases as magnification increases.

Answer 17

true
- that’s why we increase the light using iris diaphragm at for higher magnification lenses

Question 18

why do we do GRAM STAINS?

Answer 18

• it separates bacteria into 2 categories based on cell wall structure
  gram negative = red
  gram positive= purple