MICRO-PARA Flashcards by Keishea Mae Cosep

S. epidermidis differentiated from S. aureus

growth on MSA

S. aureus ferments mannitol while
S. epidermidis does not ferments mannitol

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gram pos cocci in clusters that grows on BA from medium to large with golden yellow colonies and beta-hemolytic colonies

S. aureus

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A coagulase positvie

S. aureus

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What agar thay differentiate strep from staph

Catalase test

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What test measures bound coagulase

Slide coagulase

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It is responsible for converting fibrinogen to insoluble fibrin in measuring free coagulase

Staphylothrombin

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What differentiate S. epidermidis from S. saprophyticus

S. saprophyticus resistant to Novobiocin

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pinpoint, translucent gray colonies
large and deep zone of beta hemolysis on BA

Streptococci pyogenes

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Orgasnism identification for Streptococci pyogenes and their positive reaction

Bacitracin Disc Test - sensitive
PYRase Test - Pyrrolidonylarlamidase - Pink or cherry red color
Direct antigent test - clumping

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Virulence factor For Streptocci Pyogenes that is responsible for resistant of phagocytosis and intracellular killing

M protein

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Virulence factor of S. pyogenes that mediates adherence to host cells

Lipoteichoic acid

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Virulence factor in S. pyogenes that responsible for the rash in scarlet fever

Pyrogenic exotoxins

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common causes of S. pyogenes

Streptococcal sore throat

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Commonly cause damage to the mitral valve
nodules caused by rheumatic fever

Poststreptococcal infxn can be seen in Rheumatic fever

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What test that differentiate S. pyogenes from other strep

(-) CAMP test

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bullseye appearing colony
small and diffused zone of beta hemolysis

Streptococcus agalactiae

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(-) Bacitracin
(-) TMP SXT
(+) CAMP Test

S. agalactiae

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(+) Hippurate Hydrolysis = deep purple color

S. agalactiae

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A nonhemolytic organism that grows in the presence of bile and hydrolyse esculin to esculetin, can withstand a higher salt conc. than other gram (+) cocci

enterococcus

e.bovis, e. faecalis

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translucent, gray, rough margin with umbonate center

Viridans Streptococci

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Commonly associated with dental caries

Streptococcus bovis = type of viridans.

basta dental - viridans - S. bovis

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gram (+) diplococci
capsule
alpha hemolysis
—> Translucent colonies w/c resembles water droplet

S. pneumoniae

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(+) Bactiracin Test
(-) TMP SXT
(-) CAMP

S. pyogenes

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Test that identifies S. pneumoniae

Optochin Inhibition test
Bile solubility test
capsular swelling test

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What org that has the ability of bacterial cells to lyse in the presence of bile salt

S. pneumoniae

What test is performed when bacteria is put in an antiserum, capsule can be seen to swell. other name?

Capsualr swelling test Neufeld Quellung reaction

gram (-) diplococci kidney-bean shaped cocci

Neisseria gonorrhoeae

culture medium of choice for Neisseria gonorrhoea

Thayer-Martin medium

What is added in the Thayer-Martin Medium to: 1. inhibit gram + org 2. inhibit gram - org 3. inhibit swarming proteus 4. inhibit fungus

1. vancomycin 2. Cholesitn 3. Trimethoprim 4. Lecithin

What color is produced when an enzyme added with tetramethyl paraphenylenediamine dihydrochloride? What is that enzyme What is the organism where we perform ni

purple and black color colonies Cytochrome oxidase Neisseria gonorrhoeaa

Term when gonorrhea passes in infants through an infected birth canal

Ophthalmia neonatorum

found in small amounts in nasopharynx, then it travels into the bloodstream then to the brain, causing Meningococcus

Neisseria meningitis

ocauses fever, headache, stiff neck, increase level of PMNs in spinal fluuid

N. meningitis

What is called when N. meningitidis found in the blood stream that has abrupt onset of spiking fever, chills, asthralgias and muscle pains

Meningococcemia

T/F. N. gonorrhoeae is easily destroyed by heat or outside temperature so it must inoculate iimediately into the medium or use transport packs

true

What is the confirmatory test performed in Neisseria in order to differentiate the two? What are its results?

Carbohydrate Fermentation Test N. gonorrhoeae ferments glucose only N. meningitidis ferments Glucose and Maltose

"Bamboo rod" appearance

Bacillus anthracis

spore-forming gram + bacilli that is positive for Lecithinase test

Bacillus cereus

meningitis esp. in children

Haemophilus influenzae

whooping cough

bordetella

What haemophilus is referred when it ferments glucose, sucrose and fructose

Haemophilus parainfluenzae

What haemophilus os reffered when it does not ferments any carbohydrate

Haemophilus ducreyi

An organism that has mousy or bleach-like odor in CA

H. influenzae

A term referred when nodes tend to become matted together forming an oval mass

bubo

Term used that is commonly occurs in untreated px, the skin will break down leading to sinus formation and discharge pus. this can be seen to px that has an ulcerative genital

Suppuration

"school of fish" appearance in gram-stained smear

Haemophilus ducreyi

Px with Bordetella pertussis, together with whooping cough, a px can also have __

Subconjunctival hemorrhage

T/F. Spx used in diagnosing Bordetella pertussis is nasopharynx. Org can only be seen during paroxysmal and convalescent stage

false. catarrhal and paroxysmal

Mercury Drop colonies

Bordetella pertussis

Medium used in Bordetella pertussis when Mercury drop colonies is seen

Bordet-gengou medium

What vaccine is used to prevent Pertussis toxin

Acellular vaccine DPT vaccine

Transport medium used when inoculation of spx at the px's bedside is not evident. This px experienced subjunctival hemorrhage and whooping cough w/c is the most evident

Regan-Lowe transport medium

Identify Virulence factor of Bordetella pertussis 1. causes vasoconstriction and ischemic necrosis 2. facilitates attachment of bacteria to ciliated epithelial cells 3. decreased chemotaxis and phagocytosis of bacteria 4. promotes attachment of bacteria to host cells 5. Inhibits all cilia movement and regeneration of damaged cells 6. lymphocytosis, sensitixation of histamine, activation of insulin production that leads to hypoglycemia

1. Dermonecrotic toxin 2. Filamentous hemagglutinin 3. Adenylate cyclase oxin 4. Agglutinogens 5. Tracheal toxin 6. Pertussis toxin

What is the pigment that responsible for the pink colonies of Serratia marcescens?

prodigiosin

colored colonies with greenish metallic sheen on EMB

E. coli

large mucoid colonies

Klebsiella penumoniae

fish-eye colonies

Enterobacter aerogenes/cloacae

Jet black colonies on bismuth sulfite agar

Salmonella typhi

Primarily a pediatric disease with most infxn in children 6 mos - 10 yrs . this also referred as Bacillary dysentery

Shigella

Gram neg coccobacilli with variable motility fermwents lactose and other sugars with the production of acid and gas index of fecal contamination

E. coli

Common cause of acute gastroenteritis associated with consumption of improperly cooked seafood

Vibrio parahaemolyticus

responsible for rapidly progressive wound infxn after exposure to contaminated seawater and septicemia after consumption of raw oyster

Vibrio vulnificus

Primary habitat of Helicobacter pylori

Hman gastric mucosa