micro practical

Question 1

sterile cotton swab

Answer 1

used to take clinical specimen

Question 2

bacteriological loops

Answer 2

used to isolate & subculture of bacteria
1. metal
2. plastic

Question 3

anaerobic jar

Answer 3

to grow bacteria in the absence of free oxygen

Question 4

disposable petri dish

Answer 4

for solid media preparations

Question 5

Autoclave

Answer 5

used for sterilization

Question 6

Hot Air Oven

Answer 6

For sterilization

Question 7

Incubator

Answer 7

To grow bacteria at optimal
temperature

Question 8

Bunsen burner

Answer 8

To sterile metal instruments

Question 9

Optical (light) microscopy

Answer 9

• bright
• dark
• fluorescence
• phase contrast

Question 10

electron microscopy

Answer 10

• Transmission
• scanning

Question 11

types of preparation

Answer 11

• liquid
• solid

Question 12

slide wit 8 steps

Answer 12

appearance of bacteria using Gram stain

Question 13

dark spotty slide

Answer 13

Gram-positive bacteria (Staph. aureus)

Question 14

light orange spotty slide

Answer 14

Gram-negative bacteria (E. coli)

Question 15

Transport Media

Answer 15

to transport clinical specimen

Question 16

simple ordinary media

Answer 16

liquid: peptone water
used as base for sugar fermentation

Question 17

solid

Answer 17

nutrient agar
base of diff types of media

Question 18

enriched media

Answer 18

cooked meat medium
To grow anaerobic bacteria

Question 19

Blood agar

Answer 19

suitable for the growth of most
bacteria

Question 20

Loeffler’s serum

Answer 20

suitable for the growth of C.diphtheriae.

Question 21

Chocolate agar

Answer 21

used for Haemophillus and
pathogenic neisseria.

Question 22

Lowenstein-Jensen medium

Answer 22

used for the cultivation of tubercle bacilli.

Question 23

MacConkey s medium

Answer 23

used for the cultivation of enteric bacteria

Question 24

Total count techniques

Answer 24

• direct
• turbidity

Question 25

Viablecount technique

Answer 25

- Pour plate method - Miles and Misra