micro test 1 Flashcards

1
Q

characteristic of cellular life: uptake of nutrients from the environment within the cell, and elimination of wastes into the environment.

A

metabolism

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2
Q

characteristic of cellular life: chemicals from the environment are turned into new cells under the direction of preexisting cells

A

reproduction

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3
Q

characteristic of cellular life: formation of a new cell structure such as a spore, usually part of a cellular life cycle

A

differentiation

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4
Q

characteristic of cellular life: cells communicate or interact primarily by means of chemicals that are released or taken up

A

communication

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5
Q

characteristic of cellular life: living organisms are often capable of self propulsion

A

movement

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6
Q

characteristic of cellular life: cells contain genes and evolve to display new biological properties.

A

evolution

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7
Q

microbiologist: first to describe microorganisms, coined the term cells on viewing cork

A

Robert Hooke

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8
Q

microbiologist: first to describe bacterial cells, “wee animalcules”

A

leeuwenhoek

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9
Q

microbiologist: founder of the science of bacteriology, defined bacteria, developed initial classification scheme for bacteria

A

Cohn

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10
Q

microbiologist: microbes in lactic acid fermentation, yeast in alcohol fermentation, disproof of spontaneous generation, developed vaccines

A

Pasteur

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11
Q

microbiologist: developed methods for pure cultures, postulates for determination of the etiological agents of disease, discovered cause of tuberculoses

A

Koch

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12
Q

microbiologist: germ theory of disease

A

Koch

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13
Q

microbiologist: enrichment culture, nitrogen fixation, first to describe viruses

A

Beijerinck

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14
Q

microbiologist: chemolithotropy

A

Winogradsky

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15
Q

— typically contain a single circular chromosome

A

prokaryotes

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16
Q

— normally have pairs of several linear chromsomes

A

eukaryotes

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17
Q

prokaryotes are — (haploid/diploid?)

A

haploid

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18
Q

circular, extrachromosomal, genetic elements

A

plasmids

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19
Q

are plasmids essential under standard growing conditions

A

no

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20
Q

the – is a membrane enclosed structure that contains the chromosomes in eukaryotic cells

A

nucleus

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21
Q

the — is the aggregated mass of DNA that constitutes the chromosome of cells of bacteria and archaea

A

nucleoid

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22
Q

– get energy from chemicals

A

chemotrophs

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23
Q

— get energy from the sun

A

phototrophs

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24
Q

— get energy from organic chemicals

A

chemoorganotrophs

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25
--- get energy from inorganic chemicals
chemolithotrophs
26
--- get carbon from organic compounds
heterotrophs
27
all chemoorganotrophs are --
heterotrophs
28
--- use completely oxidized carbon as their carbon source
autotrophs
29
-- producers are the autotrophs, organisms that produce organic carbon dioxide
primary
30
large group of bacteria that are gram negative
proteobacteria
31
group of bacteria that includes endospore-formers bacillus and clostridium, gram +
gram positive bacteria
32
group of bacteria that are oxygenic phototrophs
cyanobacteria
33
group of stalked bacteria
planctomycetes
34
group of bacteria with long, thin, spiral shapes
spirochetes
35
group of bacteria that are highly resistant to radiation
deinoccoci
36
group of archaea that are extreme heat dwelling
pyrolobus
37
group of archaea that are salt loving
halobacteria
38
group of archaea that have no cell wall and grow at a pH <1
thermoplasma
39
mutualistic relationship between a fungus and a phototroph
lichen
40
--- makes up about 70% of the weight of a typical bacterial cell (molecule)
water
41
non-informational biological macromolecules (2)
polysaccharides, lipids
42
informational biological macromolecules (2)
nucleic acids, proteins
43
pentoses are used for --
nucleic acids
44
hexoses are used for --
storage molecules, cell wall monomers
45
cell wall precursor
N-acetylglucosamine
46
do different glycosidic bonds result in different properties in polysaccharides
yes
47
macromolecule: amphipathic, aggregate to form membranes, energy rich bonds, good for storage
lipids
48
pyrimidines
cytosine, thymine, uracil
49
purines
adenine, guanine
50
macromolecule: informational, components are C5 carbohydrates, nitrogenous base, and phosphate
nucleic acid
51
what attaches to 1' carbon of a ribose
nitrogen base
52
what attaches to the 3' carbon of ribose
phosphodiester bond/other nucleotides
53
what happens at the 2' carbon of a ribose
OH or H
54
macromolecule: peptide bonds, structural complexity, primary, secondary, tertiary, and quaternary structures
proteins
55
protein secondary structures
alpha helices, beta sheets
56
microscopy: type of microscopy that includes bright-field, phase contrast, dark field, and fluorescence
light
57
-- microscopy allows visualization of samples with sufficient contrast. using wet mounts, live cells can be examined
bright field
58
-- increases contrast in cells by absorbing transmitted light. it can kill cells and distort their morphologies
staining
59
differential stain
gram stain
60
-- microscopy allows visualization of samples that have poor contrast (low absorption), but have different refractive indicies. converts phase differences
phase contrast
61
--- microscopy where light admitted to the objective is only that reflected by, or defracted through the sample
darkfield
62
--- microscopy where polarized light is split into distinct beams, and then generate a 3d image
differential interference contrast (DIC)
63
-- microscopy: no lenses, not a form of light microscopy, image is generated by a stylus that scans the specimen and the image is generated by a computer, image is 3d
atomic force
64
-- microscopy: laser illumination is directed through a thin plane of focus, digital images collected on a computer can be stacked to produce a 3d view of a complex, thick specimen
confocal scanning laser
65
microscopy: uses electrons instead of light waves for increased resolution, about 1000x smaller than a light microscope
electron
66
--- microscopy: uses electromagnets to focus electron beam through the sample, can examine internal detail
transmission EM
67
--- microscopy: for viewing surfaces only, specimen is coated in an e- dense film, e- are reflected from sample to detector and assembled into an image
scanning EM
68
--- linkages are common in bacterial and eukaryotic phospholipids
ester
69
--- linkages are common in archaeal phospholipids
ether
70
--- compose the hydrophobic portion of bacterial membranes
fatty acids
71
polymers of isoprene called --- compose the hydrophobic portion of archaeal membranes
phytanyl
72
planar lipids that are rare in prokaryotes
sterols
73
planar lipids that are found in bacteria but not known in archaea
hopanoids
74
membrane function: prevents leakage and functions as a gateway for transport and nutrients into and out of the cell
permeability barrier
75
membrane function: site of many proteins involved in transport, bioenergetics, and chemotaxis
protein anchor
76
membrane function: site of generation and use of the proton motive force
energy conservation
77
transport driven by the energy in the proton motive force
simple
78
transportation where chemical modification of the transported substance is driven by phosphoenolpyruvate
group translocation
79
transportation where periplasmic binding proteins are involved and energy comes from ATP
ABC system
80
simple transporter with one direction transport and a single molecule
uniporter
81
simple transporter with two direction transport with two molecules
antiporter
82
simple transporter with one direction transport with two molecules
symporters
83
glucose, mannose, and fructose are all controlled by the phosphotransferase system, which is the --- method of transporting
group translocation
84
do all cells have cell walls
no
85
archaea lack ---, but have cell walls made of different polysaccharides
peptidoglycan
86
do gram positives or gram negatives have a larger peptidoglycan layer
gram positives
87
allow for permeability across the outer membrane, create channels that traverse the membrane
porins