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Microbe Flashcards

1
Q

E. COLI characteristics

A

GRAM - Bacilli, Lactose Fermentator (Beta-Galactose), Encapsulated, motile (explains Asc UTI’S), UREASE -

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2
Q

Where E.coli Grows

A

-Eosin Methylene Blue agar (black colonies)
-MaConkey

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3
Q

What bacteria Grows in Eosin methylene Blue agar

A

E.coli

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4
Q

E.Coli virulence Factors ass. (3)

A

**Fimbria **(cystitis and Pyelonephritis)
K capsule (meningitis and pneumonia)
LPS Endotoxin (septic shock)

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5
Q

Thrombocytopenia, Hemolytic anemia, Azotemia ass with Hamburger eating 1 day ago. .

A

STEC (Shiga Like Toxin producing E.coli). = Entero-Hemorargic E.coli (EHEC)
Schistocytes….

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6
Q

Why female get UTI more common

A

Shorter urethra+ closer to anal canal.

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7
Q

STECT (E.coli) special characteristic

A

DOSENT fermented SORBITOL

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8
Q

0157:H7

A

EHEC ,ass HUS E.coli

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9
Q

How do you differentiated between Staph Saprophyticus and Epidermais

A

Saprophytycus is NovoBiocin resistant (or negative sensative).

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10
Q

Travelers diahrrhea ass with?

A

-Entero-Toxigenic E.Coli
-Heat labile and Heat stable Enterotoxin–>inflamation of intestine–>**Watery diarrhea. **

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11
Q

Entero-Pathogenic E.Coli (EPEC)

A

-<2yr old, NO toxin produced-->causes Intestinal epi flattening by going intracellular and damaging cytoskeleton–>DECR absorption water and nutrients–>watery diarrhea.

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12
Q

Uropathogenic E.Coli (UPEC) virulence factors

A

-P Fimbriae-->helps in ascending throught kidney
-Type 1 Pilli–>helps in attachement on epitheleal cells.
-alpha and Beta Hemolysin–>lysis of cells in urinary tract.

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13
Q

What serotype ?

Dysuria,Incr urinary frequence and flank pain//On culture you see GRMA- bacilli with motile and is encapsulated.

A

Uropathogenic-E.Coli (UPEC)

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14
Q

Describe

A

-Image on right shows sensitivity towards novobiocin (Stap. Aureus and Epidermadis)
-Image on left has resistance.
-This test used to differentiate between staphyloccocus organisms.

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15
Q

-GRAM+ in clusters,coagulase -, Novobiocin resistant,urease +
-Ass sexually active female in her 20’s…

A

-Staph Saprophyticus.

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16
Q

Common risk factor for all microbes that are UREASE+.

A

-Urease + converts urea–>ammonia + CO2—>INCR pH (alkalanization)–>promotes formation of struvite stones (made up off ammonia+phosphate+magnesium)

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17
Q

Klebsiella,Staph. saprophyticus, Proteus mirabilis have in common?

A

-All are urease +.

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18
Q

-Priamary cause of UTI in females?
-Secondary

A

-E.COLI
-Staph saprophyticus.

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19
Q

Lactose fermentador and NOT urease +

A

E.coli

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20
Q

DIAGNOSIS and caharacteristics

A

-Proteus Mirabilis (Ripple=SWARMING)
-GRAM- Bacilli,urease+,aggresivly motile,POLYMORPHIC Hydrogen sulfate** NON-LACTOSE FERMENTADOR**–>differentiate between E.Coli.
-Ass with old people Hospitalized with catheter placement.

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21
Q

What microbes are polymorphic?

A

Proteus mirabilis and H.Influenza.

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22
Q

DIAGNOSIS

-Exposure to catheter forms “swarm cells” (100-1k flagella–>aggressive motile)–>moves along catheter until reaches epi.–>use Fimbriea to attach–>rel. Hemolysin.

A

Proteus Mirabilis

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23
Q

Triple Sugar Iron Test used for what?

A

-Used for diagnosis of Proteus mirabilis–>has Hydrogen Sulfate.

24
Q

Fishy odor?

A

-Proteus Mirabilis.

25
Grape-like-odor?
Pseudomonas Aerginosa
26
Where do you see?
-Urease+ microbes. -Staghorn stones.
27
Enterococcous species.
28
Dianosis
Diapper rash with satellite cells -Candida Albicans
29
Diagnosis
Trematones (Schistisoma Hematobium) -Transmited via water in infected snails. -Penetrates skin.
30
Identify
Right:Staghorn stone Left:stuvite stone -Both ass with urease+ organism.
31
Ecthyma Gangrenosum ass...
Pseudomonas Aruginosa -Rapidly progrresive necrotic cutaneous lesion.
32
-GRAM-Bacilli,non-lactose fermentador, oxidase+ Mucoid colonizes that are blue-green.
-Psuedomonas Aeruginosa
33
-GRAM- Bacilli non lactose fermentador ass with Pneumonia in patients with Cystic Fibrosis.
**Pseudomonas Aeruginosa** -Mucopolysacharide capsule.
34
P.Aeruginosa Virulenece Factors
-Phospholipase C (lysis membranes) -Endotoxin (septic shock) -**Exotoxin A (EF2 inhibitor)**
35
P.Aeruginosa related diseases.
-Sepsis,otitis externa, pneumonia, UTI (catheter patients),ecthyma gangrenosum,IV drugs users
36
What can we assume if you get hospital acquiered Pseudomonas Aeruginosa?
-Multi-drug resistant.
37
-GRAM+ cocci in chains, Gamma Hemolytic, 6.5% NaCl solutin grows and PYR +
Enterococcous species (fecalis)
38
Patient had a **appendectomy last week** and today presents with UTI. -Most likely bacteria?
Enterrococus species.
39
3 Infections associated with Enterococcous species
-**UTI, Infective Endocarditis**, Biliarytract infections.
40
Candida Albicans charactristics and related immunosupressive states (4)?
-Dimorphic Fungi, psuedohyphae+ yeast 20C* -Opportunistic infections (Diabetus,Inhaled corticosteroids, AIDS, chemotherapy,Children).
41
Candida 3 mayor infections
-**Vulvovaginitis, Diapper rash and Pseudomembranous candidiasis** ( witish cheese apperance)
42
Presentation of vulvovaginitis..
-Dysuria, Purulent discharge, Itching.
43
Diagnsosis
-Candida Albicans
44
What increases risk for Disseminated Candidiasis?
Neutropenia
45
Aspergillosis
-Ass with Farmers (grain farmers),remodeling hosuses->Aspergilloma in TB,incr risk for Hepatocellular carcinoma, Invasive disease,Bronchioctasis. -Acute angles and secrete conidia. -People at risk are neutropenic
46
Risk factor for candida UTI
Catheter placement-->biofilm production
47
Describe Helminths...
-Worms that are multi-cellular. -Nematodes,Tematones, Cestones.
48
Cercaria
-Infective form of type of tematone (**schistosoma hematobium)**
49
Schistosoma Hematobium related with secretion form where?
-Snails-->secretes cercaria-->penetrate human skin-->forms egg-->promote I.S to respond in a chronic manner. -Dysuria,Hematuria, **incr risk of squamous cell carcinoma of Badder**,hepatosplenomegaly,granuloma formation.
50
What 2 cytokines are important to know for Schistosoma Hematobium and why?
IL4 and IL13--> intense granuloma and fibrosis formation.
51
Praziquantel??
Used for tematones infect -Incr Ca++ permeability.
52
Most comm honeymoon cystitis -What test will contraindicate E.coli?
E.Coli>Staph saprophyticus -Nitrate - will be staph. saprophyticus
53
What antibiotics do you give in Hospital acquired Enterrococus UTI? -Why?
-Vancomycin+Aminoglycosides**-**->due to being very resistant (comm antibiotics will not work). -Enteroccocus species are the most resistant in streptoccocus.
54
Klebsiella Pneumonie descriptons -V.F
-GRAM - Rod, polysacharide capsule, urease +, non motile, mucoid colonizes, lactose fermentador, grows in MacConkeys agar -capsule, Pilli, LPS, saderophores (need Iron )
55
What does Maconckey Agar tells you and what does it have?
-If bacteria is lactose fermentador -Has lactose + pH sensative.
56
-Lobar Pneumonia ass with alcohol overuse -->aspiration-->lung abscess and patient with diabetus mellitus. -Also causes UTI with struviate stones.
Klebsiella
57
Organism that are urease +
P (Proteus) Cryptoccocus H.Pylori Ureaplasma Nocardia Klebsiella Staph Saprophyticus Staph epidermis

RENAL (17 decks)

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