Microbiology Flashcards

1
Q

What did Cyanobacteria do for the earth

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2
Q

What is the three-domain tree

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3
Q

What is the two-domain tree

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4
Q

What did Louis Pasteur do

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5
Q

How did Louis Pasteur defeat the idea of spontaneous generation

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6
Q

What did Robert Koch do

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7
Q

Why has there been a reduction in infectious disease

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8
Q

Why is the threat of infectious diseases still around

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9
Q

What is the WHO list of 12 resistant bacteria and their type of antibiotic resistance

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10
Q

What are the characteristics of bacteria

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11
Q

How are organisms classed

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12
Q

How are prokaryotes named

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13
Q

What are the characteristics associated with these bacterial shapes

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14
Q

What are some examples of spherical bacteria

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15
Q

What are some examples of rod-shaped bacteria

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16
Q

What are some examples of spiral shaped bacteria

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17
Q

What are the characteristics of streptococcus pyogenes

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18
Q

What are the characteristics of staphylococcus aureus

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19
Q

What are the characteristics of bacillus subtilis and bacillus anthracis

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20
Q

What are the characteristics of neisseria gonorrhoeae

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21
Q

What are the characteristics of escherichia coli

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22
Q

What are the 2 pathways to synthesise macromolecules in bacteria

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23
Q

What are the components of bacteria cells

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24
Q

What are the 2 sources of energy used by different types of bacteria

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25
Q

What are the 2 sources of carbon used by different types of bacteria

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26
Q

What are the two types of chemotrophs

A

Chemolithotrophs and chemoheterotrophs

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27
Q

What are the two types of chemolithotrophs and what are their sources of carbon

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28
Q

what are chemoheterotrophs source of carbon

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29
Q

What are some examples of chemolithotrophs

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30
Q

What are the mechanisms of these bacteria cell types

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31
Q

What are the catalytic and genetic functions in a bacterial cell during growth

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32
Q

What is the process of bacterial growth/cell division

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33
Q

What is the generation time

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34
Q

What is the role of the protein FtsZ

A

Determine cell size
Involved in septum formation at the midcell, initiating cell division

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35
Q

What happens if there is lots of nutrients available in a bacteria cell before cell division

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36
Q

What happens if there is little nutrients available in a bacteria cell before cell division

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37
Q

Why if FtsZ only localised in the mid cell

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38
Q

How does bacteria grow

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39
Q

How do you calculate the number of cells growing exponentially in a bacteria culture

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40
Q

How do you calculate generation time

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41
Q

How do you calculate specific growth rate

A

v =division rate

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42
Q

How do you calculate the division rate of bacteria

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43
Q

What are the phases of bacterial growth

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44
Q

What is turbidity (optical density)

A

Amount of light scarcity

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45
Q

What are the cons of measuring optical density to determine growth of a bacterial culture

A
46
Q

What is the main con of using log viable organisms to measure growth of a bacterial culture

A

You have to wait for the bacteria to grow before

47
Q

What is the advantage of using optical density to measure the growth of bacteria in a culture

A

You can get an immediate reading

48
Q

What occurs in the lag phase of bacterial growth in a batch culture

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49
Q

What happens in the exponential phase of bacterial growth in a batch culture

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50
Q

What happens in the stationary phase of bacterial growth in a batch culture

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51
Q

What is sporulation

A

spores are a type of cell that bacteria produce during sporulation which can resist high temp

52
Q

What are the two ways samples of bacteria cultures areproduced

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53
Q

What is the effect of increasing temp on bacterial growth

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54
Q

What is the effect of decreasing temp on bacterial growth

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55
Q

What happens to bacterial growth at the minimum, maximum and optimum temperatures

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56
Q

What is the process of cell staining

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57
Q

What is the basic structure of the cell envelope of gram negative bacteria

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58
Q

What is the basic structure of the cell envelope of gram positive bacteria

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59
Q

What is the process of gram - staining procedure

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60
Q

What is the difference between gram- positive and gram- negative bacteria

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61
Q

Label the parts of this diagram of gram positive bacteria

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62
Q

Label the parts of this diagram of gram negative bacteria

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63
Q

What phospholipids are found in the cell envelope of gram positive and gram negative bacteria

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64
Q

What types of proteins are round in gram-positive and gram-negative bacteria

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65
Q

What is the peptidoglycan layer and what are its components

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66
Q

What is the basic structure of peptidoglycan

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67
Q

What is the general information for peptidoglycan structure in bacteria

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68
Q

What is the difference between the structures of arches and bacteria

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69
Q

What do ß lactam antibiotics do

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70
Q

What do glycopeptidases do

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71
Q

What are teichoic acids in gram positive bacteria

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72
Q

What is the role of teichoic acids in gram positive bacteria

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73
Q

Draw the structure of a cell envelope of a gram negative bacterium and label it

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74
Q

What is the permeability of the outer membrane of gram negative bacteria

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75
Q

How do porins affect the permeability of the outer membrane of gram negative bacteria

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76
Q

What is the structure of the LPS (lipopolysaccharide) of gram negative bacteria and lipid A

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77
Q

What is meant by polar or ( monotrichous or amphitrichous)

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78
Q

What is meant by lophotrichous flagella

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79
Q

What is peritrichous bacteria

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80
Q

What is borrelia burgdorferi

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81
Q

Label this diagram of the organisation of gram negative bacterium flagellum

A
82
Q

What type of secretion system is the flagella

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83
Q

How is the L, P and M ring transported

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84
Q

How is the flagellum assembled in a gram negativebacterium

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85
Q

How are the proteins which make up the rod, hook and filament as well as the MS ring transported

A
86
Q

What is one possible model for the rotation of the motor of the flagella in bacteria

A

Motor not rotor*

87
Q

What is the basal body of the flagella made up of

A
88
Q

What are the a components of the motor

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89
Q

How is the rotor powered

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90
Q

What are the characteristics of the flagella

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91
Q

How is swimming motility of a bacterium measured

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92
Q

What are the 2 types of movement for bacteria with peritrichous flagella and how do they occur

A
93
Q

What are the 2 types of movement for bacteria with polar flagella and how do they occur

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94
Q

What kind of movement do peritrichous bacteria exhibit when there is no attractant

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95
Q

What kind of movement do peritrichous bacteria exhibit when there is an attractant

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96
Q

What is chemotaxis

A

Movement of a motile cell in a direction corresponding to a gradient of increasing or decreasing concentration of a particular substance

97
Q

How can chemotaxis be investigated

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98
Q

How do bacteria mediate chemotaxis when there is no attractant

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99
Q

How do bacteria mediate chemotaxis when there is an attractant

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100
Q

How do bacteria detect an attractant or repellant

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101
Q

What are MCPs and how do they work

A

methyl accepting chemotaxis proteins =mcp

102
Q

What happens after an MCP interacts with CheW and CheA

A
103
Q

What happens when molecules bind to an MCP

(Sensory adaptation and phosphorylation explained in more detail)

A
104
Q

What are pili

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105
Q

What are type IV pili and what do they do

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106
Q

How is type iv pili assembled

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107
Q

Have does twitching motility occur

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108
Q

How is the swarming motion of bacteria investigated

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109
Q

How is swimming and swarming motility investigated

A

Use of wild type and mutant
Either pili or flagella mutated or both
Can see whether swimming or swarming inhibited

110
Q

Summarise the characteristics of the 3 types of bacterial motility

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111
Q

What are fimbriae

A