MICROBIOLOGY Flashcards
(5 cards)
1
Q
What 4 things are needed for gram staining
A
Crystal violet
Iodine
Alcohol
Safranin
2
Q
Why is crystal violet used in gram staining
A
To binds onto the peptidoglycan layers, so all bacteria can stain purple
3
Q
Why is iodine used in gram staining
A
To bind the crystal violet strongly to the peptidoglycan layers
4
Q
Why is alcohol used in gram staining
A
Remove the unbound crystal violet and lipopolysaccharide
5
Q
Why is Safranin used in gram staining
A
To stain gram negative bacteria red
