Microbiology Diversity Flashcards

(44 cards)

1
Q

Humans have over ___ trillion microbes residing in and on the body. Microbes outnumber human cells in what ratio?

A

100

3:1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What two types of bacteria does gram staining categorize into?

A

Gram Positive - Bacteria have much thicker cell walls, holding the dye in better (purple)
Gram Negative - Bacteria have much thinner cell walls, allowing the dye to leak (pink)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Using the MacConkey agar, does E.coli produce lactose? is it gram negative or positive?

A

It does produce lactose as it is red

It is gram negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Branches shift depending on the genomes sequences used

A

tree of life

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Gene sequencing is the main method used today to determines. Before it was mutations

A

relatedness between organisms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

The most common fungi is ______

A

yeast

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

coccus means

A

spherical

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Why are archaea hard to study

A

They leave in extreme places, are small, and are hard to find

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

We have never found an archaea that casues ______ in humans

A

disease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Mycology is the study of what?

A

Fungi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Hyphae and chitin makes fungi

A

distinct

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

A virus is an infectios agent that has RNA or DNA as it’s core

A

ds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

How many bacteria can be in one teaspoon of fertile soil?

A

100 million to 1 billion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Microbes have also been found through the Earth’s _________, up to _ miles above ground

A

troposphere, 9

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

How can bacteria in the sky influence the weather?

A

Nucleating ice crystal formations and forming ran drops that eventually bring the bacteria back down to the ground

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

How do microbes in Antarctica stay alive?

A

They scavenge hydrogen, carbon monoxide, and carbon dioxide form the air

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Researchers found almost ______ different bacteria living under 800 meters of ice in the west Antarctic

A

4000

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Marine microbes make up to __ to __% of the ocean’s biomass

19
Q

______ _______ perform most of the world’s oxygen and also aid the in global cycling of carbon, nitrogen, phosphorous, and other nutrients

A

Marine microbes

20
Q

Where can most microbes in humans be found?

A

Skin, hair, respiratory system, and digestive tract

21
Q

How can microbes aid or harm humans?

A

Some help with digestion

Some causes lung infections or creates plaque on teeth

22
Q

Where can microbes be found?

A

Almost every environment on Earth

23
Q

Microbes can be both ______ and _________

A

prokaryotes, eukaryotes

24
Q

Which kingdoms have microbes?

A

Bacteria, archaea, protists, fungi, and sometimes viruses

There are also some microscopic animals

25
What 3 categories do we use to categorize microbes?
Physical Characteristics Chemical Characteristics Genetic Phylogeny showing relationships between organisms
26
Microscopy enables ________ __________ of microbes
visual identification
27
What is Gram staining?
A technique to visualize bacteria
28
What are examples of physical characteristics that can be identified with microscopes?
prokaryote vs eukaryote size, shape, color, cell wall characteristics immobile vs mobile
29
Describe how the petri dish and agar came to be?
- Scientists tried to cultivate microbes on various substrates like potatoes, egg white, and meat. These lacked the right nutrients - Robert Koch discovered that aqueous humor from rabbit eye was effective, but hard to get - Koch tried to use gelatin, but that liquefies at 37C - His assistant Angelina Hesse discovered that agar was effective - His other assistant Julius Petri designed a shallow, covered dish for the agar
30
What is a colony?
A collection of multiple identical organisms
31
What are some physical characteristics of colonies?
shape, elevation, margin, surface features, opacity, pigmentation
32
What are some environmental conditions that affect microbe growth?
Temperature, surface material, humidity, PH, and the composition of nutrients
33
How can we grow specific microbes?
Carefully controlling growth conditions for the desired microbe
34
What are some examples of chemical characteristics that can be used to identify microbes?
Autotrophic (only need CO2) vs heterotrophic (need organic carbon) Chemical requirements for life processes Resistance to antibiotics, etc.
35
How does phylogenetic analysis work? Who proposed this, and when?
It works by comparing sequences of genes or proteins to identify genetic relationships between organisms Proposed by Emile Zuckerkandl and Linus Pauling in 1965
36
When and who classified archaea separately from bacteria due to differences in their rRNA genes?
1977 - Carl Woese and George E. Fox
37
Carl Woese, Otto Kandler and Mark Wheelis later proposed to reclassify organisms from the 2 empire system to the _______ ______ system of life
3 domain | From Prokaryotes and Eukaryotes to Bacteria, Archaea, and Eukarya
38
Describe bacteria
Single celled prokaryotic microbes Range of 0.5 to 5 um in length Distinguished by cell wall, their shape, and genetic makeup Can be mobile or immobile. (The presence of flagella and/or cilia help distinguish bacteria)
39
Rank the 3 domains of life from most diverse to least diverse
Bacteria Eukarya Archaea
40
Describe Archaea
- Single celled prokaryotes (0.1 to 15 um in diameter) - Generally similar in size and shape to bacteria (but also includes shapes like squares) - First discovered in extreme environments (extremophiles) - Salt lakes, pH 0 hot springs, 122C ocean thermal vents - Can also live in more mild conditions (mesophiles) - More related to Eukarya than bacteria (but endosymbiotic theory suggests otherwise) - Most abundant ammonia-oxidizing organisms in soils and accounts for 20% of the microbes present in picoplankton - Remove 10-25% of global methane emissions - Nanoarchaea are the smallest known living cell (1/100 size of E. Coli) with the smallest genome (112 kilobases)
41
Describe Kingdom Fungi
- Christiaan Hendrik Persoon considered the founder of modern mycology - Includes yeast, mold and mushrooms - Are heterotrophs - Have chitin in their cell walls - Grows hyphae underground. (2-10 um diameter, several cm length) - The mushroom is the reproductive structure containing spores in its gills (which germinate to make more hyphae - Oregon fungal colony is 2400 years old and span 2200 acres
42
Describe protists
- Mostly unicellular, but some are multicellular - 3 general categories: animal like heterotrophs (amoeba), plant like autotrophs (green algae), fungus like autotrophs (slime mold)
43
Describe Algae
- Variety of shapes (spherical, rod, club, spindle) - Some motile - Contains chlorophyll - Can be unicellular and microscopic or multicellular and up to 120 m - Some organized as filaments of cells attached end to end - Occur in colonies
44
Describe viruses
- Small infection agent that can only reproduce itself inside the cells of a living host. (animals, plants, microbes) - First one studied was tobacco mosaic virus - 1887, Dmitri Ivanovsky discovered that the disease was caused by extremely small infection agents, capable of passing through filters - 1898, Martinus Beijerinck replicated above results and called it a virus - In the same year, Friedrich Loeffler and Paul Frosch identified the first animal virus - Early 20th century, Frederick Twort discovered bacteriophages, viruses that infect bacteria - Origin unclear because no fossil records. Evidence based on molecular analyses of the viral genomes and also viral genetic material that integrates into the reproductive cells of its host - Could pre-date divergence of life into 3 domains