MICROBIOLOGY Quiz Exercise 2

Question 1

in their natural state is difficult to see

Answer 1

Bacteria

Question 2

They are colorless and transparent when observed under the microscope

Answer 2

Bacteria

Question 3

How do you visualize bacteria in order to see them

Answer 3

Various stains
Dyes

Question 4

used in microbiology that are salts and are acidic, basic, or neutral?

Answer 4

Stains

Question 5

They are composed of positive and negative ions

Answer 5

Stains

Question 6

it is one of the ions that impart color?

Answer 6

Chromophore

Question 7

In acidic dyes it has a negative charge that reacts with the positively charged components of the cell, like the cytoplasm?

Answer 7

Chromophore

Question 8

Acidic dyes are also referred to as?

Answer 8

cytoplasmic stains.

Question 9

They have a chromophore that is positively charged and stains the negatively-charged components of the cell, like the nucleic acids in the nucleus

Answer 9

Basic dyes

Question 10

What are basic dyes referred to?

Answer 10

nuclear stains

Question 11

They are composed of consisting of mixtures of acidic and basic dyes?

Answer 11

Neutral stains

Question 12

What are the the three kinds of staining techniques used to apply acidic and basic dyes?

Answer 12

Simple staining
differential staining
special staining

Question 13

what are kinds of simple stains?

Answer 13

safranin
methylene blue
crystal violet

Question 14

What is the use of simple stains?

Answer 14

to highlight the entire organism

Question 15

Used to distinguish the reaction of bacteria to a particular stain

Answer 15

Differential stains

Question 16

What are example of differential stains?

Answer 16

Gram stain

Question 17

It is a stain that is very useful in microbiology

Answer 17

Gram stain

Question 18

It classifies bacteria into either gram positive or gram negative?

Answer 18

Gram stain

Question 19

They are used to color a specific part of a microorganism such as the endospore, flagellum capsule?

Answer 19

Special stains

Question 20

Special stains are used to color a specific part of a microorganism such as?

Answer 20

endospore
flagellum
capsule

Question 21

it is a film of the organisms on a clean slide is prepared, dried and fixed what is this called?

Answer 21

Smear

Question 22

What are the materials used in exercise 2?

Answer 22

Slides
Inoculating loops
Alcohol lamps
Droppers
Gram’s iodine

Question 23

What are the stains

Answer 23

Malachite Green
Safranin
Methylene Blue
Gentian Violet

Question 24

What are the bacterial cultures used?

Answer 24

Staphylococcus aureus
Bacillus subtilis
Escherichia coli

Question 25

What are materials used in Preparation of bacterial smear?

Answer 25

Glass slide sterilized inoculating loop alcohol lamp

Question 26

in simple staining how many minutes do you cover the smears in the stain?

Answer 26

1 minute

Question 27

What are the stains used in gram staining?

Answer 27

Crystal violet safranin

Question 28

What do you apply if you finish applying crystal violet stain for 30 seconds and rinsing in with tap water?

Answer 28

Gram's iodine solution

Question 29

What is used to decolorize the stain?

Answer 29

acid alcohol

Question 30

how many seconds do decolorization take in gram staining?

Answer 30

10-30 seconds

Question 31

what is used on counterstaining?

Answer 31

safranin for about 30 seconds

Question 32

What stains are used in using endospore staining?

Answer 32

malachite green safranin

Question 33

What bacterias are used in endospore staining?

Answer 33

bacillus subtilis

Question 34

What color does endospore appear?

Answer 34

Green while other parts of the cell are red

Question 35

In simple staining what is used for examination?

Answer 35

LPO,HPO,OIO

Question 36

In gram staining what is used for examination?

Answer 36

HPO,OIO

Question 37

In endospore staining what is used for examination?

Answer 37

HPO,OIO