Microscope

Question 1

enables us to observe and identify the minute organisms

Answer 1

microscopy

Question 2

use of microscopy in microbio

Answer 2

• identify microorganisms by observing their features
• different type of microscope serve different functions to characterize microorganisms
• understanding the biology of microorganisms

Question 3

Types of microscope

Answer 3

1. Compound
2. Darkfield
3. Electron
4. Fluorescence
5. Phase Contrast

Question 4

has two lenses (objective and ocular) with source of light that can either be natural and artificial

Answer 4

compound microscope

Question 5

LPO

Answer 5

10x

Question 6

HPO

Answer 6

40x

Question 7

Oil Immersion Objective

Answer 7

100x

Question 8

Scanning Objective

Answer 8

4x

Question 9

What are the sources of light of compound microscopes?

Answer 9

visible light (artificial or natural light)

Question 10

has a special field type of condenser that scatters the light

Answer 10

darkfield microscope

Question 11

illuminate unstained specimen against a dark background

Answer 11

darkfield microscope

Question 12

ues beam of electrons (light) to illuminate specimens

Answer 12

electron microscope

Question 13

provides a high resolution of biological and non-biological samples

Answer 13

electron microscope

Question 14

uses ultraviolet light to illuminate speciments

Answer 14

fluorescence microscope

Question 15

detection of specific structures within a cell with the use of a specific fluorescent dye

Answer 15

fluorescent microscope

Question 16

what type of objective is best when viewing under fluorescence microscope

Answer 16

OIO

Question 17

best to use in observing microorganisms in their natural state

Answer 17

phase contrast microscope

Question 18

has a special condenser that enables the observation inside the cell

Answer 18

phase contrast microscope

Question 20

what is the best slide to use in phase contrast microscope?

Answer 20

hanging drop slide

Question 21

stain or unstain

compound microscope

Answer 21

stain/not stain

Question 22

stain or unstain

darkfield microscope

Answer 22

not stained

Question 23

stain or unstain

electron microscope

Answer 23

stain/not stain

Question 24

stain or unstain

fluorescence microscope

Answer 24

stained

Question 25

# stain or unstain phase contrast microscope

Answer 25

unstained

Question 26

# type of parts of the microscope eyepiece

Answer 26

mechanical

Question 27

# type of parts of the microscope diopter adjustment

Answer 27

mechanical

Question 28

# type of parts of the microscope revolving nosepiece

Answer 28

mechanical

Question 29

# type of parts of the microscope objective lenses

Answer 29

magnifying

Question 30

# type of parts of the microscope mechanical stage

Answer 30

mechanical

Question 31

# type of parts of the microscope stage clips

Answer 31

mechanical

Question 32

# type of parts of the microscope diaphragm

Answer 32

illuminating

Question 33

# type of parts of the microscope condenser

Answer 33

illuminating

Question 34

# type of parts of the microscope illuminator (light source)

Answer 34

illuminating

Question 35

# type of parts of the microscope head

Answer 35

mechanical

Question 36

# type of parts of the microscope arm

Answer 36

mechanical

Question 37

# type of parts of the microscope coarse adjustment knob

Answer 37

mechanical

Question 38

# type of parts of the microscope fine adjusment knob

Answer 38

mechanical

Question 39

# type of parts of the microscope light switch

Answer 39

mechanical

Question 40

# type of parts of the microscope base

Answer 40

mechanical

Question 41

# type of parts of the microscope ocular lenses

Answer 41

magnifying

Question 42

When viewing under scanning and LPO, what adjustment knob should you use?

Answer 42

coarse adjustment knob

Question 43

when viewing under HPO and OIO, what adjustment knob should you use?

Answer 43

fine adjustment knob

Question 44

distance between objective and slide

Answer 44

working objective

Question 45

# true or false the lower magnification, the higher working distance

Answer 45

true

Question 46

# true or false the higher magnification, the higher working distance

Answer 46

false | magnification is inversely proportional to working distance

Question 47

number that expresses the ability of a lens to resolve fine detail in an object being observed

Answer 47

numerical aperture

Question 48

# true or false the higher the magnification, the higher numerical aperture

Answer 48

true

Question 49

# true or false the higher the magnification, the lower numerical aperture

Answer 49

false | magnification and NA are directly proportional with each

Question 50

# What is the numerical aperture of: 4x (scanning)

Answer 50

0.1

Question 51

# What is the numerical aperture of: 10x (LPO)

Answer 51

0.25

Question 52

# What is the numerical aperture of: 40x (HPO)

Answer 52

0.65

Question 53

# What is the numerical aperture of: 100x (OIO)

Answer 53

1.25

Question 54

# color of objective lenses scanning

Answer 54

red

Question 55

# color of objective lenses LPO

Answer 55

yellow

Question 56

# color of objective lenses HPO

Answer 56

blue

Question 57

# color of objective lenses OIO

Answer 57

white

Question 58

range of distance for which the specimens (objects) is imaged with an acceptable sharpness on the image plane

Answer 58

depth of focus

Question 59

# true or false depth of focus is inversele proportional to NA

Answer 59

true

Question 60

depth of specimen layer which is in sharp focus at the same time

Answer 60

depth of field

Question 61

# true or false depth of field is inversely proportional to NA

Answer 61

true

Question 62

ability of microscope to distinguish s specimen/sample

Answer 62

resolution

Question 63

# true or false resolution is inversely proportional to NA and directly proportional to depth of field

Answer 63

true