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Microbiology Lab > Aseptic Technique > Flashcards

Aseptic Technique Flashcards

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1
Q

all organisms are descendants of one organisms

A

pure culture

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2
Q

In working with microorganisms, we must have:

A

sterile-nutrient-containing-medium
method of transferring organisms (inoculum)

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3
Q

method of transferring organisms from pure culture to sterile medium

A

inoculum

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4
Q

techniques to maintain aseptic condition

A

aseptic technique

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5
Q

performing lab work that greatly reduces the risk of contamination

A

aseptic technique

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6
Q

What is the proper flame?

A

small blue cone

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7
Q

What are the different techniques in transferring the inoculum into tubes?

A

Aseptic Pipetting
Slant to Slant
Slant to Broth
Broth to Slant
Deep Agar Stab

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8
Q

When do you use inoculating needle?

A

Deep Agar Stab

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9
Q

What arm would you use in holding the stock culture, agar and broth?

A

non-dominant hand

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10
Q

what hand will you use in holding the inoculating needle/loop?

A

dominant hand

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11
Q

How do you unscrew the test tubes?

A

use little finger and ring finger

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12
Q

Why must the loop be cooled?

A

to prevent from killing microorganisms that are not resistant to heat

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13
Q

In getting culture, what must the amount be?

A

small

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14
Q

If agar surface is wet, what must be done?

A

wipe with sterile swab

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15
Q

How deep will you stab agar?

A

1 cm

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16
Q

how long will you incubate culture?

A

48 hours

17
Q

What are the techniques in transferring the inoculum to petri plate?

A

Streak Plating
Spread Plating
Pour Plating
Dilution

18
Q

spreading source material over an agar surface until one microorganism at a time fall off the loop

A

streak plating

19
Q

single type of microorganism that originated from a single cell

A

colony

20
Q

how many bacteria/mL can you see in pond water?

A

10^5 to 10^6

21
Q

how many bacteria/mL can you see in soil?

A

10^8

22
Q

how many bacteria/mL can you see in turbid culture of Escherichia coli?

A

10^7

23
Q

how many bacteria/mL can you yield from an agar plate?

A

10^3 to 10^5 colonies

24
Q

method employed to plate a liquid sample for the purpose of isolating or counting the bacteria present in that sample

A

spread plate technique

25
Q

A perfect spread plate technique will result to visible and isolated colonies of bacteria that are evenly distributed in the plate and are countable. (t or f)

A

True

26
Q

most commonly applied for microbial testing of foods or isolating and identifying microbial flora present in environmental sample

A

spread plating

27
Q

What are the glasswares required in spread plating?

A

screw capped tube
sterile pipettes
glass spreader

28
Q

What is the medium required in spread plating

A

plate count agar
nutrient agar

29
Q

How many test tubes must be prepared in dilution?

A

6 test tubes containing 9 ml of sterile distilled water

30
Q

What is the rotation angle of petri dish in spread plating

A

45 degrees

31
Q

The plate in spread plating must be incubated for:

A

37 degrees; 24 hours

32
Q

method for counting the number of colony-forming bacteria present in a liquid specimen

A

pour plating

33
Q

pour plate is more precise than streak plate (t or f)

A

true

34
Q

pour plate gives a lower count since non-heat resistant microorganisms can die in pouring molten agar. (t or f)

A

true

35
Q

optimum count of colonies in pour plating

A

30-300 colonies per plate

36
Q

The plate in pour plating must be incubated for:

A

37 degrees; 24-48 hours

37
Q

colonies on the surface will be smaller than embedded colonies in pour plating

A

false (embedded colonies will be smaller)

Microbiology Lab (7 decks)

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