Aseptic Technique Flashcards
GO FOR UNO!!!
all organisms are descendants of one organisms
pure culture
In working with microorganisms, we must have:
sterile-nutrient-containing-medium
method of transferring organisms (inoculum)
method of transferring organisms from pure culture to sterile medium
inoculum
techniques to maintain aseptic condition
aseptic technique
performing lab work that greatly reduces the risk of contamination
aseptic technique
What is the proper flame?
small blue cone
What are the different techniques in transferring the inoculum into tubes?
Aseptic Pipetting
Slant to Slant
Slant to Broth
Broth to Slant
Deep Agar Stab
When do you use inoculating needle?
Deep Agar Stab
What arm would you use in holding the stock culture, agar and broth?
non-dominant hand
what hand will you use in holding the inoculating needle/loop?
dominant hand
How do you unscrew the test tubes?
use little finger and ring finger
Why must the loop be cooled?
to prevent from killing microorganisms that are not resistant to heat
In getting culture, what must the amount be?
small
If agar surface is wet, what must be done?
wipe with sterile swab
How deep will you stab agar?
1 cm
how long will you incubate culture?
48 hours
What are the techniques in transferring the inoculum to petri plate?
Streak Plating
Spread Plating
Pour Plating
Dilution
spreading source material over an agar surface until one microorganism at a time fall off the loop
streak plating
single type of microorganism that originated from a single cell
colony
how many bacteria/mL can you see in pond water?
10^5 to 10^6
how many bacteria/mL can you see in soil?
10^8
how many bacteria/mL can you see in turbid culture of Escherichia coli?
10^7
how many bacteria/mL can you yield from an agar plate?
10^3 to 10^5 colonies
method employed to plate a liquid sample for the purpose of isolating or counting the bacteria present in that sample
spread plate technique
