Microscope

Question 1

Used in routine urinalysis

Answer 1

Bright field microscope

Question 2

• Aids in identification of cholesterol in oval fat bodies, fatty cast and crystals

Answer 2

Polarized Microscope

Question 2

Enhanced visualization of elements with low refractive indices

Answer 2

Phase-Contrast Microscope

Question 3

• Aids in identifying of T.pallidum

Answer 3

Dark-Field Microscope

Question 4

• Allows visualization of naturally fluorescent microorganism or those stained by a fluorescent dye

Answer 4

Fluorescence Microscope

Question 5

• produces a three dimensional microscopy

Answer 5

Interference Contrast Microscope

Question 6

Stained preparations are examined by means of ordinary light that passes through the specimen.

Answer 6

BRIGHT-FIELD MICROSCOPY

Question 7

defined as the smallest distance between two particles at which they can be seen as separate objects.
→ 0.2 um

Answer 7

Resolving power

Question 8

Tissue sections are usually irradiated with ultraviolet (UV) light and the emission is in the visible portion of the spectrum.

Answer 8

FLUORESCENCE MICROSCOPY

Question 9

The fluorescent substances appear brilliant on a dark background.

Answer 9

FLUORESCENCE MICROSCOPY

Question 10

Phenomenon wherein certain cellular substances are irradiated by light of a proper wavelength, they emit light with a longer wavelength.

Answer 10

Fluorescence

Question 11

Based on the principle that light changes its speed when passing through cellular and extracellular structures with different refractive indices.

Answer 11

PHASE-CONTRAST MICROSCOPY

Question 12

Uses a lens system that produces visible images from transparent objects

Answer 12

PHASE-CONTRAST MICROSCOPY

Question 13

Enables examination of unstained cells and tissues and is especially useful for living cells.

Answer 13

PHASE-CONTRAST MICROSCOPY

Question 14

allows quantification of tissue mass & Differential interference microscope

Answer 14

Interference microscope

Question 15

using Nomarski optics

Answer 15

Interference microscope

Question 16

useful for assessing surface properties of cells and other biologic objects.

Answer 16

Interference microscope

Question 17

No direct light from the light source is gathered by the objective lens.

Answer 17

DARK-FIELD MICROSCOPY

Question 18

Only light that has been scattered or diffracted by structures in the specimen reaches the objective.

Answer 18

DARK-FIELD MICROSCOPY

Question 19

equipped with a special condenser that illuminates the specimen with strong, oblique light.

Answer 19

DARK-FIELD MICROSCOPY

Question 20

Field of view appears as a dark background on which small particles in the specimen that reflect some light into the objective appear bright.

Answer 20

DARK-FIELD MICROSCOPY

Question 21

combines components of a light optical microscope with a scanning system to dissect specimens optically.

Answer 21

CONFOCAL MICROSCOPY

Question 22

Uses the fact that highly ordered molecules or arrays of molecules can rotate the angle of the plane of polarized light.

Answer 22

POLARIZING MICROSCOPY

Question 23

ability to rotate the direction of vibration of polarized light

Answer 23

Birefringence

Question 24

Based on the interaction of tissue components with beams of electrons.

Answer 24

ELECTRON MICROSCOPY

Question 25

The wavelength in the electron beam is much shorter than that of light, allowing a 1000-fold increase in resolution.

Answer 25

ELECTRON MICROSCOPY

Question 25

ELECTRON MICROSCOPY 2 KINDS

Answer 25

TEM AND SEM

Question 26

an imaging system that permits resolution around 3 nm.

Answer 26

TRANSMISSION ELECTRON MICROSCOPY

Question 26

magnifications of up to 400,000 times to be viewed in detail

Answer 26

TRANSMISSION ELECTRON MICROSCOPY

Question 27

uses the interaction of a beam of electrons with a specimen to produce an image

Answer 27

TRANSMISSION ELECTRON MICROSCOPY

Question 28

a special method of sample preparation for transmission electron microscopy -it is especially important in the study of membranes.

Answer 28

Cryofracture and freeze etching

Question 29

the electron beam does not pass through the specimen but us scanned across its surface

Answer 29

SCANNING ELECTRON MICROSCOPY

Question 30

surface of the specimen is first dried and spray-coated with a very thin layer of heavy metal (often gold) through which electrons dó not pass readily.

Answer 30

SCANNING ELECTRON MICROSCOPY

Question 31

When the beam is scanned from point to point across the specimen, it interacts with the metal atoms and produces reflected electrons or secondary electrons emitted from the metal

Answer 31

SCANNING ELECTRON MICROSCOPY