Microscope Flashcards
(12 cards)
What is the first step in using a microscope to view a cell?
Place the prepared slide with your specimen on the stage of the microscope and secure it with stage clips.
This ensures that the specimen is stable and correctly positioned for viewing.
Which objective lens should you start with when viewing a specimen under a microscope?
The lowest power objective lens (e.g., 4x).
Starting with a low power lens allows for a wider field of view and easier focusing.
What is the purpose of the coarse focus knob?
To bring the specimen into rough focus.
This is the initial focusing adjustment before fine tuning the image.
How can you enhance the illumination when viewing a specimen?
Adjust the light source or condenser for the best illumination.
Proper lighting is crucial for clear visibility of the specimen.
What is the function of the fine focus knob?
To make the image sharp and clear.
This knob allows for precise focusing after using the coarse focus.
What should you do after focusing on a specimen with a low power objective lens?
Switch to a higher power objective lens for more detail, refocusing as needed.
Higher magnification provides a closer view of the specimen’s structures.
What is a key consideration when moving the slide under the microscope?
Move the slide gently to explore different areas and always handle the microscope carefully.
This prevents damage to the slide or microscope.
What is the first step in preparing a slide with a solid specimen?
Cut or peel a very thin layer so light can pass through.
Thin samples are essential for proper light transmission and visibility.
What should you place on the center of a clean glass slide?
The thin sample.
Centering the specimen ensures that it is in the field of view when using the microscope.
What substances can be added to enhance the visibility of structures in a slide?
Water or stain (such as iodine for plant cells or methylene blue for animal cells).
Stains help to highlight different cellular structures.
How should you lower a coverslip over the specimen?
At an angle to avoid air bubbles.
Air bubbles can obstruct the view and affect the clarity of the image.
What should you do if there is excess liquid after placing the coverslip?
Gently press down if needed and wipe away any excess liquid.
This ensures a clean viewing area and prevents distortion of the image.
