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Bacteriology Lab > Microscope > Flashcards

Microscope Flashcards

(63 cards)

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1
Q

Lab Safety Regulations

A
  • Put aside all unnecessary things
  • Conduct yourself in a responsible manner
  • Follow all written and verbal instructions
  • Wear PPE; Remove after
  • Wash hands before and after
  • Wear face shields or masks and eye protection
  • Treat all specimen as biohazard
  • Tie back hair
  • Decontaminate work area before and after
  • Do not eat, drink or smoke
  • Do not place anything in your mouth
  • Minimize creation of aerosols
  • Place all infectious wastes and materials in appropriate receptacle
  • Needles should not be bent, cut or recapped.
  • When infectious fluid is spilt, clean with disinfectant and notify instructor
  • Do not use cellphone during lab
  • familiarize all safety features in the lab
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2
Q

the study of organisms that are so small they cannot be seen with the naked eye.

A

Microbiology

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3
Q

minute organisms that cannot be seen by naked eye

A

microorganisms or microbes

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4
Q

Branches of Microbiology

A 
Bacteriology
Virology
Mycology
Parasitology
Phycology
Immunology
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5
Q

study of baceria

A

Bacteriology

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6
Q

study of viruses

A

Virology

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7
Q

study of fungi

A

Mycology

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8
Q

study of protozoa and parasitic worms

A

Parasitology

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9
Q

study of algae

A

Phycology

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10
Q

study of immune response

A

Immunology

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11
Q

Categories of Microbes

A

Cellular

Acellular

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12
Q

classifications of cellular microorganisms

A

Prokaryotes

Eukaryotes

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13
Q

microbes without true nucleus

A

Prokaryotes

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14
Q

microbes with true nucleus

A

Eukaryotes

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15
Q

classification of Prokaryotes

A

Eubacteria
Cyanobacteria
Archeans

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16
Q

true bacteria

A

Eubacteria

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17
Q

blue green algae

A

cyanobacteria

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18
Q

organisms that can survive in extreme environment

A

Archeans

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19
Q

classification of eukaryotes

A 
fungi
protozoa
algae
plants
animals
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20
Q

forms of acellular microbes

A

viruses

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21
Q

Why do we need to study bacteriology?

A
  • isolate and identify
  • pathogenic microorganisms
  • to assist
  • diagnosis, management and treatment
  • of infectious disease
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22
Q

is the entrance and multiplication of a microorganism in or on a host

A

infection

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23
Q

refers to an infection with functional and structura harm to the host that usually is accompanied by signs and symptoms

A

infectious disease

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24
Q

microorganism that is capable of causing infectious disease

A

pathogen

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25
- are microorganisms residing in a particular body site | - do not generally cause infection
normal flora or commensals
26
- attacks on already debilitated host | - usually presents no danger to an individual with an intact immune system
opportunistic pathogen
27
the science of investigating small objects using a microscope
Microscopy
28
most common method used for both detection of microorganisms directly in the clinical specimens - and for the characterization of organisms grown in culture
microscopy
29
defined as the use of microscope to magnify objects too small to be visualized with the naked eye so that characteristics are readily observable
microscopy
30
- the lens at the top that you look through | - usually 10x or 15x power
ocular or eyepiece
31
used to magnify the object that is viewed from the eyepiece
objectives
32
is the distance between the front lens of the objective and the specimen on the stage
working distance
33
the higher the magnifying power,
the shorter the working distance
34
where the specimen to be examined is placed
stage
35
holds the specimen in place
stage clip
36
holds two or more objective lenses and can be rotated to easily change power
revolving nosepiece
37
other name of revolving nosepiece
turret
38
produces light to illuminate and view the specime
light source/ illuminator
39
gather light rays and focus these on the object to be examined
condenser
40
is an opaque disk with an opening can be expanded or contracted to increase or decrease the amount of light illuminating the object
iris diaphragm
41
used to begin the focus
coarse adjustment knob
42
used to finally adjust the details of a fuzzy focus
fine adjustment knob
43
Types of Light Microscopes
Brightfield Microscope Phase Contrast Microscope Darkfield Microscope Fluorescent Microscope
44
Microscopes that can be used for bacteria
Brightfield Fluorescent Darkfield
45
Microscopes that can't be used for bacteria
Electron Microscopy
46
Microscopes that can be used for fungi
Brightfield | Fluorescent
47
Microscopes that can't be used for fungi
Darkfield | Electron
48
Microscopes that can be used for Parasites
Brightfield Fluorescent Electrone
49
Microscopes that can't be used for Parasites
Darkfield
50
Microscopes that can be used for viruses
Fluorescent | Electron
51
Microscopes that can't be used for viruses
brightfield | darkfield
52
Microscopes that are limitedly used for bacteria
Darkfield
53
Microscopes that are limitedly used for parasites
Electron
54
Microscopes that are limitedly used for viruses
Electron
55
viruses are called ______ because they need a host in order to survive
obligate intracellular parasites
56
other name of pathogenic
harmful microorganism
57
common stomach commensal
lactobacillus acidophilus
58
color of scanner
red
59
color of LPO
yellow
60
color of HPO
blue
61
Color of OIO
white
62
objectives used in coarse adjustment
scanner, LPO
63
objectives used in fine adj.
HPO, OIO

Bacteriology Lab (2 decks)

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