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Flashcards in Microscope Deck (63)
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1

Lab Safety Regulations

- Put aside all unnecessary things
- Conduct yourself in a responsible manner
- Follow all written and verbal instructions
- Wear PPE; Remove after
- Wash hands before and after
- Wear face shields or masks and eye protection
- Treat all specimen as biohazard
- Tie back hair
- Decontaminate work area before and after
- Do not eat, drink or smoke
- Do not place anything in your mouth
- Minimize creation of aerosols
- Place all infectious wastes and materials in appropriate receptacle
- Needles should not be bent, cut or recapped.
- When infectious fluid is spilt, clean with disinfectant and notify instructor
- Do not use cellphone during lab
- familiarize all safety features in the lab

2

the study of organisms that are so small they cannot be seen with the naked eye.

Microbiology

3

minute organisms that cannot be seen by naked eye

microorganisms or microbes

4

Branches of Microbiology

Bacteriology
Virology
Mycology
Parasitology
Phycology
Immunology

5

study of baceria

Bacteriology

6

study of viruses

Virology

7

study of fungi

Mycology

8

study of protozoa and parasitic worms

Parasitology

9

study of algae

Phycology

10

study of immune response

Immunology

11

Categories of Microbes

Cellular
Acellular

12

classifications of cellular microorganisms

Prokaryotes
Eukaryotes

13

microbes without true nucleus

Prokaryotes

14

microbes with true nucleus

Eukaryotes

15

classification of Prokaryotes

Eubacteria
Cyanobacteria
Archeans

16

true bacteria

Eubacteria

17

blue green algae

cyanobacteria

18

organisms that can survive in extreme environment

Archeans

19

classification of eukaryotes

fungi
protozoa
algae
plants
animals

20

forms of acellular microbes

viruses

21

Why do we need to study bacteriology?

- isolate and identify
- pathogenic microorganisms
- to assist
- diagnosis, management and treatment
- of infectious disease

22

is the entrance and multiplication of a microorganism in or on a host

infection

23

refers to an infection with functional and structura harm to the host that usually is accompanied by signs and symptoms

infectious disease

24

microorganism that is capable of causing infectious disease

pathogen

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- are microorganisms residing in a particular body site
- do not generally cause infection

normal flora or commensals

26

- attacks on already debilitated host
- usually presents no danger to an individual with an intact immune system

opportunistic pathogen

27

the science of investigating small objects using a microscope

Microscopy

28

most common method used for both detection of microorganisms directly in the clinical specimens

- and for the characterization of organisms grown in culture

microscopy

29

defined as the use of microscope to magnify objects too small to be visualized with the naked eye so that characteristics are readily observable

microscopy

30

- the lens at the top that you look through
- usually 10x or 15x power

ocular or eyepiece

31

used to magnify the object that is viewed from the eyepiece

objectives

32

is the distance between the front lens of the objective and the specimen on the stage

working distance

33

the higher the magnifying power,

the shorter the working distance

34

where the specimen to be examined is placed

stage

35

holds the specimen in place

stage clip

36

holds two or more objective lenses and can be rotated to easily change power

revolving nosepiece

37

other name of revolving nosepiece

turret

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produces light to illuminate and view the specime

light source/ illuminator

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gather light rays and focus these on the object to be examined

condenser

40

is an opaque disk with an opening

can be expanded or contracted to increase or decrease the amount of light illuminating the object

iris diaphragm

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used to begin the focus

coarse adjustment knob

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used to finally adjust the details of a fuzzy focus

fine adjustment knob

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Types of Light Microscopes

Brightfield Microscope
Phase Contrast Microscope
Darkfield Microscope
Fluorescent Microscope

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Microscopes that can be used for bacteria

Brightfield
Fluorescent
Darkfield

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Microscopes that can't be used for bacteria

Electron Microscopy

46

Microscopes that can be used for fungi

Brightfield
Fluorescent

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Microscopes that can't be used for fungi

Darkfield
Electron

48

Microscopes that can be used for Parasites

Brightfield
Fluorescent
Electrone

49

Microscopes that can't be used for Parasites

Darkfield

50

Microscopes that can be used for viruses

Fluorescent
Electron

51

Microscopes that can't be used for viruses

brightfield
darkfield

52

Microscopes that are limitedly used for bacteria

Darkfield

53

Microscopes that are limitedly used for parasites

Electron

54

Microscopes that are limitedly used for viruses

Electron

55

viruses are called ______ because they need a host in order to survive

obligate intracellular parasites

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other name of pathogenic

harmful microorganism

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common stomach commensal

lactobacillus acidophilus

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color of scanner

red

59

color of LPO

yellow

60

color of HPO

blue

61

Color of OIO

white

62

objectives used in coarse adjustment

scanner, LPO

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objectives used in fine adj.

HPO, OIO