Microscopes Flashcards
Tools used to enlarge images of small objects so as they can be studied.
Microscopes
Historians credit the invention of the microscope to which Dutch spectacle makers? Around what year?
Hans and Zacharias Janssen; 1590
Most of the documented works of the time of the creation of the first compound microscope are credited to the likes of whom?
Antoine van Leeuwenhoek
and Robert Hooke
Instrument containing two lenses, which
magnifies, and a variety of knobs to resolve
(focus) the picture
Compound Light Microscope
ability to make smaller objects look
larger in contrast to their original sizes
Magnification
capability of a system to distinguish
details in a sample being viewed
Resolution
depends on the distance between two distinguishable points in a field of vision
Resolution
how we detect an object against a
background
Contrast
relative difference in the light intensity
between the image and the background it has
Contrast
Light microscopes discussed in the LG
Brightfield Microscope
Dark field Microscope
Phase contrast
Differential interference contrast
Fluorescence
Confocal
Two-photon
Creates an image by means of
directing light from a light source to
the sample or specimen being
observed
Brightfield Microscope
most commonly
used type of microscope
Brightfield Microscope
uses
two or more lenses that produces a
dark image in a light background
Brightfield Microscope
works like a brightfield microscope but has a modification in the part called the condenser
Dark field Microscope
A darkfield microscope works like a
brightfield microscope but has a modification in the part called the
condenser
shows a bright specimen on a dark background
Dark field Microscope
Types of electron microscopes
transmission electron microscope and the
scanning electron microscope
Used in studying inner morphology
of samples
Transmission Electron Microscopes
requires ultra thin slices of samples and then stained with metals like gold or palladium
Transmission Electron Microscopes
Used to study surfaces of samples
Scanning Electron Microscopes
shows the overall surface of our
specimen
Scanning Electron Microscopes
This is the platform on which the microscope slide rests and the clamp that secures the slide
Mechanical stage
control the stage front to back and the slide from side to side
Mechanical stage control knobs (x-y control knobs)
This adjusts the intensity of light
reaching the specimen under focus
Iris diaphragm