microscopes

Question 1

define magnification

Answer 1

the degree to which the size of the image is larger than the object itself

Question 2

define resolution

Answer 2

degree to which it is possible to distinguish between two objects that are very close together

Question 3

stain and colour for DNA

Answer 3

acetic orcein, dark red

Question 4

stain and colour for cytoplasm

Answer 4

eosin, pink

Question 5

stain and colour for lipids

Answer 5

sudan red, red

Question 6

stain and colour for cellulose and starch

Answer 6

iodine, blue black

Question 7

stain and colour for nucleus

Answer 7

Nile blue, blue

Question 8

how do you calculate magnification

Answer 8

size of image/actual size

Question 9

how many micrometers are in a millimetre

Answer 9

1000

Question 10

how many news in a micrometre

Answer 10

1000

Question 11

max resolution and magnification of light microscope

Answer 11

200nm, x2000

Question 12

max resolution and magnification of transmission electron microscope

Answer 12

0.2nm, x500,000

Question 13

max resolution and magnification of scanning electron microscope

Answer 13

0.2nm, x250,000

Question 14

how do you view something under a microscope

Answer 14

1-stain and attach to slide, use lowest objective lens
2-look through eyepiece, move course focus
3-move fine focus
4-change magnification and repeat

Question 15

which microscopes can view a live specimen?

Answer 15

light

Question 16

which microscopes require a stain?

Answer 16

light

Question 17

how is the beam focussed in all 3 microscopes?

Answer 17

-lens in light microscope
-electromagnet in others