Microscopes (from powerpoint)

Question 1

What is the purpose of staining?

Answer 1

Stains make stuctures visible/ increase contrast.

So you can see different organelles/ recognise different types of cells.

Question 2

What are the 2 types of stains?

Answer 2

General stains and differentiated stains.

Question 3

What are differentiated stains?

Answer 3

Stains that bind to specific cell structures, staining each differently so they can be easily identified within a single preparation.

Question 4

What is methylene blue?

Answer 4

An all purpose stain (blue)

Question 5

What is Sudan red?

Answer 5

Differentiated stain
Lipid stain (red)

Question 6

What is iodine?

Answer 6

Differentiated stain
Starch-blue/black
Cellulose- yellow

Question 7

What is magnification?

Answer 7

How many times bigger the image is compared to the actual size.

Question 8

What is resolution?

Answer 8

The minimum distance apart 2 objects must be in order for them to appear as seperate items.
(better resolution = clearer image)

Question 9

What is the type of beam in a light microscope?

Answer 9

Light

Question 10

What is the type of beam in a laser scaning (confocal) light microscope?

Answer 10

Laser light

Question 11

What is the type of beam in an electron scanning microscope?

Answer 11

Electron

Question 12

What is the type of beam in an electron transmission light microscope?

Answer 12

Electron

Question 13

What is the wavelength of a beam in a light microscope?

Answer 13

400-700nm

Question 14

What is the wavelength of a beam in a laser scanning (confocal) microscope?

Answer 14

400-700nm

Question 15

What is the wavelength of a beam in an electron scanning microscope?

Answer 15

0.004nm

Question 16

What is the wavelength of a beam in an electron transmission microscope?

Answer 16

0.004nm

Question 17

What is the magnification in a light microscope?

Answer 17

x1500-x2000

Question 18

What is the magnification in a laser scanning (confocal) microscope?

Answer 18

x1500 - x2000

Question 19

What is the magnification is a electron scanning microscope?

Answer 19

x100,000 - x500,000

Question 20

What is the magnification in a transmission electron microscope?

Answer 20

x500,000 - x2,000,000

Question 21

What is the resolution of a light microscope?

Answer 21

200nm

Question 22

What is the resolution of a laser scanning (confocal) microscope?

Answer 22

800nm

Question 23

What is the resolution of an electron scanning microscope?

Answer 23

3-10nm

Question 24

What is the resolution of an electron transmission microscope?

Answer 24

0.2-0.5nm

Question 25

How are specimens prepared for a light microscope?

Answer 25

Place on glass slide

Question 26

How are specimens prepared for a laser scanning (confocal) microscope?

Answer 26

Specimen can be tagged with fluorescent dye

Question 27

How are specimens prepared for a electron scanning microscope?

Answer 27

Speciemen coated with fine layer of metal

Question 28

How are speciens prepared for a electron transmission microscope?

Answer 28

Thinly spiced, chemically fixed by dehydration and stained.

Question 29

What image is produced from a light microscope?

Answer 29

2D colour image

Question 30

What image is produced by a laser scanning (confocal) microscope?

Answer 30

3D image

Question 31

What image is produced by a electron scanning microscope?

Answer 31

3D image with false colour

Question 32

What image is produced by a electron transmission microscope?

Answer 32

2D grey scale image

Question 33

Can a light microscope be used to view living organisms?

Answer 33

Yes

Question 34

Can a laser scanning (confocal) microscope be used to view living organisims?

Answer 34

Yes

Question 35

Can an electron scanning microscope be used to view living organisms?

Answer 35

No

Question 36

Can an electron transmission microscope be used to view living organisms?

Answer 36

No

Question 37

What is the special viewing condition for electron microscopes?

Answer 37

Viewed in a vacuum, stained with metal salts.