Microscopic Methods Flashcards
Illuminating source is the light.
Light Microscope
Specimen preparation takes usually few
minutes to hours.
Light Microscope
It has low resolving power (0.25µm to
0.3µm).
Light Microscope
It has a magnification of 500X to 1500X.
Light Microscope
The object is 5µm or thicker.
Light Microscope
Vacuum is not required.
Light Microscope
There is no need of high voltage electricity.
Light Microscope
There is no cooling system.
Light Microscope
Filament is not used.
Light Microscope
Radiation risk is absent.
Light Microscope
It is used for the study of detailed gross
internal structure
Light Microscope
Illuminating source is the beam of
electrons.
Electron Microscope
Specimen preparation takes usually takes
few days.
Electron Microscope
All lenses are electromagnetic
Electron Microscope
It has high resolving power (0.001µm),
about 250 times higher than light
microscope.
Electron Microscope
It has a magnification of 100,000X to
300,000X.
Electron Microscope
The object is 0.1µm or thinner.
Electron Microscope
Vacuum is essential for its operation.
Electron Microscope
High voltage electric current is required
50,000 Volts and above
Electron Microscope
It has a cooling system to take out heat
generated by high electric current
Electron Microscope
Tungsten filament is used to produce
electrons.
Electron Microscope
Image is received in zinc sulphate
fluorescent screen or photographic plate.
Electron Microscope
It is used in the study of external surface,
structure of atom and very small particles.
Electron Microscope
