Microscopic techniques Flashcards

1
Q

optical microscopy

A

use visible light and a system of lenses to get magnified images of small samples
400 - 700 nm in wavelength

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2
Q

wavelength (m)

A

distance from a point in a cycle to the corresponding point in the next
peak to peak, trough to trough

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3
Q

frequency (Hz)

A

number of vibrations of a given wavelength in one second
longer wavelength = lower frequency

velocity = freq x wavelength

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4
Q

Absorption

A

all light enters, nothing exits (thermal, electrical, chemical changes)

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5
Q

reflection

A

all light is turned back into the incident material
rough surface = more diffuse reflection, less specular reflection

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6
Q

refraction

A

ray bent when passing between 2 transparent materials = change in velocity
affected by incident angle and wavelength of incident ray and surface

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7
Q

refractive index

A

sin angle1 / sin angle2 = n1/n2 = v1/v2

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