Microscopy

Question 1

micrometer: 1μm =

Answer 1

10^-6 m

Question 2

nanometer: 1nm =

Answer 2

10^-9 m

Question 3

picometer: 1pm =

Answer 3

10^-12 m

Question 4

RBCs _____ in diameter

Answer 4

7μm

Question 5

sizes of bacteria, protozoa, and fungi are expressed in (measurement)

Answer 5

micrometers μm

Question 6

the sizes of viruses are usually expressed in

Answer 6

nanometers

Question 7

resolving power

Answer 7

resolution: smallest distance b/w 2 adjacent points at which they will
appear distinct & separate→ detail & clarity of image

Question 8

Compound light microscope

Answer 8

illumination source: visible light
magnification: 1000x
resolving power: 1000x greater than the naked eye (0.2 μm)

Question 9

ocular lens (eye piece) in CLM has a magnification of

Answer 9

10x

Question 9

ocular lens (eye piece) in CLM has a magnification of

Answer 9

10x

Question 10

bright-field microscope

Answer 10

bright background
Used to observe morphology of MO’s in both living and non-living state

Question 11

Dark-field microscope

Answer 11

dark background, observe morphology of unstained MO’s (living state

Question 12

phase-contrast microscope

Answer 12

Observe living unstained MOs in their naturalstate→ internal
details & organelles (e.g.,mitochondria, lysosomes, & Golgi body) can be seen clearly.

Question 13

Fluorescence microscope

Answer 13

UV light, dark background, and non-living MO
used to show that Abs stained with
fluorescent dye have combined with specific Ags

Question 14

Electron microscope (EM)

Answer 14

can not be used for living MO’s
use electron beam
greater resolving power

Question 15

if wavelength increases, the resolving power

Answer 15

decreases

Question 16

Transmission Electron Microscope has a resolving power of _____ and magnification of _______

Answer 16

0.2 nm, 200,000x (which is 200 times greater than compound microscope)

Question 17

scanning electron microscope: resolving power:
magnification:

Question 17

scanning electron microscope resolving power:
magnification:

Answer 17

20nm, 10,000

Question 18

Resolving power of TEM is ____ times greater than SEM and ____ times more CLM

Answer 18

100, 1000