microscopy Flashcards

(36 cards)

1
Q

what is used to magnify and see specimens what the naked eye cannot see

A

microscope

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2
Q

who discovered the microscope

A

ZACHARIAS JANSSEN

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3
Q

who is the father of the microscope?

A

ANTONI VAN LEEUWENHOEK

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4
Q

microscope with one lens, Similar to this will be a Magnifying lens but we don’t call this a microscope because it is only a lens

A

SIMPLE MICROSCOPE

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5
Q

two or more lens,

A

COMPOUND LIGHT MICROSCOPE

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6
Q
  • Has space for your hands to move
  • It doesn’t have a stage
  • light source at the top and bottom
A

STEREOMICROSCOPE

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7
Q

what allows u to see whatever is in here and to see whatever is in focus thus allowing you to trace viewed specimens

A

drawing tube

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8
Q

microscope that is only used by experts and depends on electron beams to show you images

A

electron microscopy

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9
Q

allows you to view the surface

A

sem image or scanning electron microscope

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10
Q

shows you which structure of the specimen is more dense than the other, allows u to differentiate or identify the different parts or the internal structures of the specimen

A

tem image or transmission electron microscope

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11
Q

this is the real 3d, allows u to view surface and internal structures

A

atomic force microscopy

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12
Q

similar to SEM image but instead of electron, it used laser. When you target a certain structure, you could select a certain die, subject it to the specimen, and view it under this microscope

A

confocal microscopy

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13
Q

it is where u view the specimen and is 10x

A

eyepiece

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14
Q

has a scanner that is 4x, LPO, HPO, AND OIO

A

Objective lenses

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15
Q

Low power objective =

A

10x

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16
Q

high power objective =

17
Q

oil immersion objective =

18
Q

it can revolve or rotate and lets u view the specimen in diff. magnification

19
Q

this is used when there are different or multiple lenses

A

total magnification

20
Q

how do u get the total magnification?

A

eyepiece mag. x objective mag.

21
Q

Where the specimen is placed
where the unknown length is

22
Q

keeps specimen in place

A

stage clips/holder

23
Q

connects the eyepiece and nose piece

24
Q

supports the whole microscope

25
elevates and lifts, moves the stage, is bigger than other knob
coarse adjustment knob
26
makes u zoom in and out, is the smaller knob
fine adjustment knob
27
can reflect and gives u light. Makes the light pass through the condenser
light source / mirror
28
it condenses light and make it much more brighter - When you have higher magnification, the light should be much more focused.
condenser
29
the bg is bright or white
white filmthe
30
the bg is dark
black film
31
we see this in electron microscope because it shows u which is much more darker
face contrast
32
-placed directly on the eyepiece -Known value, needs calibration - it will look the same for all magnification
ocular micrometer
33
placed on the stage -Larger magnification = decrease in spaces -Smaller magnification = increase in spaces -can be magnified depending on the objective lens
stage micrometer
34
computation of calibration factor
CF = (Sm) (10m) / (0m)
35
computing for actual size
(0m)(CF)
36
actual size ocular
number of spaces X CF