Microscopy

Question 1

What is the definition of magnification?

Answer 1

How many times the real image is compared to the size of the actual image

Question 2

What is the definition of resolution?

Answer 2

The ability to distinguish two or more objects from each other

Question 3

What is the equation for overall magnification?

Answer 3

Eyepiece magnification x objective magnification = overall magnification

Question 4

What is the ratio of cm to mm to um to nm?

Answer 4

1cm = 10mm = 10 000um = 10 000 000nm

Question 5

Describe the features of an electron microscope

Answer 5

Higher magnification and resolution
Resolving power is up to 0.2nm
Enables us to see individual cells and structures in greater detail
More expensive
Allows us to see images in 3D

Question 6

Describe the features of a light microscope

Answer 6

Lower magnification and resolution
Resolving power is 200nm
Less expensive

Question 7

List the steps in the microscopy required practical

Answer 7

1. Insert prepared slide onto stage + secure with stage clips
2. Turn on lights and select lowest power objective lens (produces lowest magnification x4)
3. Look through eyepiece + adjust coarse adjustment knob to move stage up and down
4. Adjust focus with fine adjustment knob until image is clear
5. To magnify an image further, select a higher power objective lens (x10)

Question 8

What is the equation for magnification ?

Answer 8

Magnification = image size / actual size