Microscopy

Question 1

Latin meaning small

Answer 1

Micro

Question 2

Latin meaning to look at

Answer 2

Skropos

Question 3

How many times an image appears larger
Ability to enlarge an image

Answer 3

Magnification / Magnifying Power

Question 4

The shortest distance between two distinct points on a specimen.
Ability to distinguish two close objects as distinct.

Answer 4

Resolution / Resolving Power

Question 5

Magnification formula

Answer 5

M = Image size / Actual size

Question 6

Total Magnification formula

Answer 6

M (T) = Objective x ocular

Question 7

Resolution formula

Answer 7

r = 0.61 (550 nm)/NA

Question 8

Lenses that increase magnification of the specimen.

Answer 8

Objectives

Question 9

Adjusts stage movement by large increments.

Answer 9

Coarse Adjustment Knob

Question 10

Adjusts stage movement by small increments.

Answer 10

Fine Adjustment Knob

Question 11

The lens or lenses closest to your eye, used for magnification.

Answer 11

Eyepiece

Question 12

A rotating cylinder that holds multiple objective lenses

Answer 12

Nosepiece

Question 13

A thin, transparent slide used to cover the specimen for protection and mounting.

Answer 13

Coverslip

Question 14

Product of objective and ocular lens magnifications.

Answer 14

Total magnification

Question 15

Scanner magnification

Answer 15

4x

Question 16

LPO magnification

Answer 16

10x

Question 17

HPO magnification

Answer 17

40x

Question 18

OIO magnification

Answer 18

100x

Question 19

Used to measure the diameter of the field of view.

Answer 19

Ruler method or FOV diamter

Question 20

Ocular lens magnification

Answer 20

10x

Question 21

What does light microscopes use to form an image?

Answer 21

Visible light

Question 22

LPO resolution

Answer 22

1.34 micrometer

Question 23

OIO resolution

Answer 23

0.268 micrometer

Question 24

Supporting parts of a microscope

Answer 24

Eyepiece, Arm / Handle, Stage clip, Coarse adjustment, Fine adjustment, Switch, Draw tube, Revolving nosepiece / dust shield, Stage, Filter holder, Base

Question 25

Illuminating parts of a microscope

Answer 25

Illuminator Condenser Iris diaphragm

Question 26

Magnifying parts of a microscope

Answer 26

Eyepiece Objective lenses

Question 27

Adjustment knob used for scanner and LPO.

Answer 27

Coarse adjustment

Question 28

Adjustment knob used for HPO and OIO.

Answer 28

Fine adjustment

Question 29

Part that concentrates light

Answer 29

Condenser

Question 30

Part that improves light color

Answer 30

Filter holder

Question 31

Heaviest and largest part

Answer 31

Base

Question 32

What are the field of view measurements for different objectives?

Answer 32

Scanner: 4500 micrometers LPO: 1800 micrometers HPO: 450 micrometer

Question 33

Measurement of microscopic images

Answer 33

Micrometry

Question 34

How do details of specimen vary with magnification?

Answer 34

Least detailed: Scanner Lesser: LPO More detailed: HPO Most detailed: OIO

Question 35

How does the area of field of view change with magnification?

Answer 35

Smallest: OIO Smaller than LPO: HPO Bigger than HPO: LPO Biggest: Scanner

Question 36

How does brightness of image change with magnification?

Answer 36

Dimmest: OIO Dimmer than LPO: HPO Brighter than HPO: LPO Brightest: Scanner

Question 37

What is the working distance for different objectives?

Answer 37

Scanner: 25 mm LPO: 6.7 mm HPO: 0.6 mm OIO: 0.14 mm

Question 38

RBC width

Answer 38

7.5 micrometer

Question 39

WBC width

Answer 39

5 - 15 micrometer

Question 40

Two main categories of WBC

Answer 40

Granulocytes Agranulocytes

Question 41

WBC in bacterial infection, 9-12 micrometer

Answer 41

Neutrophils

Question 42

WBC in parasitic infections, allergies, collagen diseases, spleen, CNS, 10-14 micrometer

Answer 42

Eosinophils

Question 43

WBC in chickenpox, sinusitis, diabetes, myxedema, polycythemia, 8-10 micrometer

Answer 43

Basophils

Question 44

WBC in viral infections and inflammation, 12-15 micrometer

Answer 44

Monocytes

Question 45

WBC in diverse infections and immune responses, 5-17 micrometer

Answer 45

Lymphocytes

Question 46

Ability to enlarge an image

Answer 46

Magnifying Power