microscopy Flashcards
(13 cards)
How does a light microscope work?
Light passes through a specimen usually mounted on a glass slide.
What are the conditions of the specimen for a light microscope?
It must be thin so light can pass through it and so that a layer of cells are visible, be stained so structures are visible.
Why are smaller structures not visible under a light microscope?
They do not have a high resolution.
How does an electron microscope work?
They use a beam of electrons focused by electromagnets.
What are some advantages of an electron microscope?
They have a shorter wavelength and therefore provide a higher resolution, a higher magnification they still have a high resolution.
What are the two types of electron microscope?
Transmission and scanning.
Which type of electron microscope is the deflection of electrons?
Transmission.
Which type of electron microscope is the reflection of electrons?
Scanning.
Which type of electron microscope has a higher resolution?
Transmission.
Which type of electron microscope produces images of external structures?
Scanning.
Which type of electron microscope produces a 3D image?
Scanning.
Which type of electron microscope must have a very thin specimen?
Transmission.
What is the equation to calculate magnification?
Magnification = Image size ÷ Actual size.
