Microscopy Flashcards
(29 cards)
What is the maximum magnification of light microscopy?
x2000
Light microscopy provides lower magnification compared to electron microscopy.
What type of samples can be observed using light microscopy?
Living samples
Light microscopy allows for the observation of living samples in color.
What is a disadvantage of light microscopy?
Lower resolution
Light microscopy offers less detail compared to electron microscopy.
What is the maximum magnification of electron microscopy?
× 10,000,000
Electron microscopy provides much higher magnification than light microscopy.
What type of samples can be observed using electron microscopy?
Dead samples
Electron microscopy produces images in black and white.
What is a key advantage of electron microscopy?
Higher resolution
Electron microscopy provides more detail compared to light microscopy.
What is the first step in using a microscope?
Place the object or specimen onto a small sheet of glass called a slide
This process is referred to as mounting.
What is done to the specimen after mounting it on a slide?
Cut a very thin slice and add stain
Staining helps to highlight the features of the specimen.
What is the purpose of a cover slip in microscopy?
To protect the sample
The cover slip is placed over the stained specimen after mounting.
What is the function of the pair of clips in microscopy?
To secure the slide to the stage
This ensures the specimen remains in place while observing.
What is the first adjustment to make after placing the slide on the stage?
Turn the coarse focus until the slide is away from the objective lens
This initial adjustment helps to avoid damaging the slide or lens.
What should be done after turning the coarse focus?
Turn until the shortest objective lens is over the slide
Starting with the shortest lens allows for easier focusing.
What is the next step after using the eyepiece to view the specimen?
Slowly turn the coarse focus until the object is seen clearly
This step is crucial for obtaining a clear image.
What is the final step in obtaining the greatest detail of the specimen?
Turn the fine focus until the object is in greatest detail
The fine focus allows for precise adjustments in clarity.
Fill in the blank: The _______ is used to mount the specimen for microscopy.
slide
The slide is essential for observing samples under a microscope.
Fill in the blank: Light microscopy allows for observing samples in _______.
color
This is a key feature that differentiates it from electron microscopy.
Fill in the blank: Electron microscopy produces images in _______.
black and white
This is due to the nature of electron interactions with samples.
What is the first step in preparing a meroscopy slide?
Peel off a thin layer of skin from the inner surface of a piece of onion.
How should the thin layer of onion be placed on the slide?
Use forceps to put this thin layer onto a slide, making sure the layer is flat.
What should be added to the onion tissue on the slide?
Put a drop of iodine solution onto the onion tissue.
How should the coverslip be placed onto the slide?
Carefully lower a coverslip onto the slide by placing one edge on the slide and using forceps to lower the other edge.
What should be done with excess liquid on the slide?
Use a piece of paper to soak up any excess liquid.
How should the slide be positioned on the microscope?
Put the slide onto the microscope, using the clips.
Which objective lens should be used first?
Use the lowest power objective lens x4.
