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Tissues of the body > Microscopy > Flashcards

Flashcards in Microscopy Deck (17)
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1
Q

What is a tissue?

A

A collection of cells specialised to perform a particular function

2
Q

What is the size of most human cells?

A

10-20 um

3
Q

What is a biopsy?

A

It is the removal of a small piece of tissue from an organ or part of the body for microscopic examination

4
Q

Which tissues is a transvacular biopsy used on?

A

Heart and liver

5
Q

Which method of biopsy involves scrapping tissue with a special spoon?

A

Curretage biopsy

6
Q

What are the 6 types of biopsy?

A

Smear, Curretage, Direct incision, Needle, Endoscopic, Transvacular

7
Q

Which steps of tissue fixation cause shrinkage artefacts?

A

Rehydration and dehydration

8
Q

What is the purpose of tissue fixation?

A

It allows tissues to be viewed under a slide

9
Q

What are the two common fixatives?

A

Glutaraldehyde and formaldehyde

10
Q

Haematoxylin stains what and what colour?

A

It stains the acidic components of the cell such as RNA and DNA purple

11
Q

Eosin stains the basic components of the cell such as cytoplasmic proteins or extracellular fibres. What colour does it stain them?

A

It stains them pink

12
Q

The periodic Acid-Schiff is a pink stain. What does it stain?

A

The carbohydrates and glycoproteins.

13
Q

What is the advantage of using dark field microscopy?

A

You can use live and unstained samples. This is used for detection of syphilis.

14
Q

What is the advantage of phase contrast microscopy?

A

It gives an enhanced image of unstained cells

15
Q

What is the main advantage of confocal microscopy?

A

You can obtain a 3d image from a series of 2D images

16
Q

What is histology?

A

It is the study of the structure of tissues by means of special staining techniques combined with light and electron microscopy

17
Q

What is the diameter of most human red blood cells?

A

7.2um