Microscopy and Histometry

Question 1

Describe the pathway light travels from light source to the eye for polarising microscopes

Answer 1

light soucre-> condenser lense-> polariser-> specimen-> analyser-> objective lens-> eyepiece

Question 2

Which special feature does light pass through in a dark field microscope?

Answer 2

Dark field condenser with a central opaque disc

Question 3

Which special feature does light pass through in a polarising microscope?

Answer 3

Polariser- below specimen stage

Question 4

Which special feature does light pass through in a fluorescent microscope?

Answer 4

Excitation filter followed by a dichroic mirror then finally an emission filter

Question 5

Which special feature does light pass through in a phase contrast microscope?

Answer 5

phase platea and a condenser annulus

Question 6

Which special feature does light pass through in a interferance microscope?

Answer 6

beam splitter, interferacne rings/ optical compensators

Question 7

Which common optical illusions can affect our judgement when validating tissue sections

Answer 7

1. Chromatic aberration- colour fringing cn blur tissue structures
2. Out of focus blur- tisues can appear distorted
3. Colour perception variability- differences in colour perception due to lighting

Question 8

How is the stage micrometre and eyepiece micrometre (graticule) combination used to measure structures in tissue sections?

Answer 8

allows for measurement of objects at the microscopic level by using the collaborative scale in the eyepiece in conjunction with a known reference scale on the microscope