Microscopy and Structures Flashcards Preview

Unit 1 - Molecular and Cellular Principles of Medicine > Microscopy and Structures > Flashcards

Flashcards in Microscopy and Structures Deck (15)
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Conventional Light Microscopy

~200 nm resolution, may be used to study whole unstained living cells, limited subcellular detail - cells and tissues can be fixed and stained as well


Fluorescence Microscopy

Special type of light microscopy that uses fluorescent dyes to detect specific macromolecules within cells. Some dyes bind directly to target while other are linked to a molecule that bind. Different excitation and emission wavelengths can be use to visualize different macromolecules in a single cell


Indirect immunofluorescence

One of the most common forms - combines fluorescent dyes w/ antibodies that bind selectively to particular macromolecules - primary antibody binds to antigen, secondary antibody binds to primary


Transmission Electron Microscopy

~2 nm resolution, used to study subcellular structures in great detail. Uses beam of elections, dark areas are electron dense.


Scanning electron microscopy

~10 nm resolution - used to view surfaces of whole cells and tissues rather than subcellular organelles - focused beam of electrons moved across specimen, creates 3D image


Plasma membrane through Electron Micrograph

think darkline demarcating the outer boundary of the cell - may be highly convoluted


Nucleus through Electron Micrograph

largest organelle in the cell - typically mottled gray w/ irregularly shaped staining (where heterochromatin is), surrounded by double membrane of nuclear envelope


Nucleolus through Electron Micrograph

large darkly staining roundish structure w/in nucleus


Nuclear envelope through Electron Micrograph

double membrane surrounding the nucleus - nuclear pores can be seen, may also be able to see ribosomes attached to cytoplasm


Rough ER through Electron Micrograph

rough ER consists of elongated flattened sacs - looks like long thin compartments that are fairly uniform in thickness, can be straight or curved, have ribosomes if rough ER


Smooth ER through Electron Micrograph

Smooth ER consists of elongated interconnected tubules - shows up as complex mixture of oddly shaped vesicles


Peroxisomes through Electron Micrograph

small round structures, 1 membrane - uniform medium gray, have an electron dense (dark) core, containing high concentration of oxidative enzymes


Lysosomes through Electron Micrograph

Darkly stained organelles that are typically round and vary in size, may be many in a single cell


Mitochondria through Electron Micrograph

round or oblong and present in large numbers - surrounded by double membrane - inner membrane is extensively folded to form cristae


Golgi apparatus through Electron Micrograph

series of flattened sacs (similar to rough ER), but typically only 4-8 sacs and a large # of vesicular structures. No bound ribosomes and lumens of golgi tend to be narrower than the ER - located near the nucleus