Midterm Flashcards
(100 cards)
golgi stain
silver nitrate staining of cells
Dendrites, as well as the cell soma, are clearly stained in brown and black and can be followed in their entire length, which allowed neuroanatomists to track connections between neurons and to make visible the complex networking structure of many parts of the brain and spinal cord
impregnating fixed nervous tissue with potassium dichromate and silver nitrate
only stains 1/10 of neurons
stains a limited number of cells at random
neuron doctrine
1917
Ramon y Cajal
It’s a list of important discoveries
• Neurons as individual non-continuous pieces
• The collateral and terminal ramifications of all axis-cylinders [axons] end in the grey matter
• the body and dendrites of the nerve cells, establishing a contact or articulation [synapses] between the receptor protoplasm [dendrites] and the final, tiny axonic branches
• the body and the protoplasmic processes participate in the chain of conduction, that is, that they receive and propagate the nervous impulse
• the nervous impulse is transmitted by contact, as in the articulations of electrical conductors, or by a kind of induction, as with induction coils
action potential definition
change in potential across the membrane of a cell
all or nothing
Rapid depolarization with inward current (Na+). Turning off inward current (Na+) Turning on outward current (K+) Refractory Period Return to Rest
seem to be responsible for thoughts, beliefs etc.
action potential mechanism
at resting phase all channels are closed
when voltage of cell is changing after certain threshold channel will open
–>chain reaction-other channels open
cell changes into certain conformation–>voltage change
this is a voltage dependent process
once enough Na+ is in the cell the na+ will sotp, the K+ channel will open and K+ will diffuse out of cell
central forces in action potential mechanism
flow of current through electrical circuit
diffusions
electrical attraction and repulsion
corpuscular view
Descartes and Borelli in 17thC
Nerves are hollow tubes
They carry “animal spirits.”
Vital spirits inflate muscles.
Muscles tug on bones.
swammerdam
Put frog muscle (b) in water in a syringe (a)
Stimulate (c) nerve to cause muscle to contract
Monitor movement of fluid (e)
No change in volume!
this means that contraction could not be a consequence of inflow of nervous fluid.
and that stim of nerve doesn’t cause the muscle to physically contract/expand
galvani
Electrical stimulation of the nerve causes muscular contraction
Severing the nerve prevents the contraction.
Laying the nerves across one another restores the contraction.
So it’s a kind of “animal electricity” but the “electricity” does not propagate instantaneously the way actual electricity does
squid giant axon
The action potential goes beyond zero; the cell becomes positive.
Not membrane breakdown (negates Bernstein’s theory)
Coordinated ionic fluxes combine.
bernstein’s membrane breakdown theory
Uses Differential rheotome to get “snapshots” of the passing negative variation (1868)
Membrane theory: The resting potential is explained by different ionic concentrations inside and outside the cell.
The “action potential” is a temporary but complete breakdown of the membrane.
later disproved by squid giant axon
rest potential
-70mV is the rest potential voltage (voltage differece of outside cell and inside cell)
equilibrium potential vs rest potential value comparison
eqm potential for Na+»_space;rest potential voltage
eqm potential for K+
equilibrium potential
the value of the membrane potential at the electrochemical equilibrium for a particular ion
an ion will be in electrochemical eqm if V(membrane)=V(ion)
voltage clamp does what?
allows you to assess the value of the conductance to different ions across the membrane at different voltages if we hold them constant
results from the voltage clamp
Note rapid time course of Na+ conductance change
Note delayed and prolonged effect on K+ conductance.
hodgkin and huxley’s question
Could voltage-dependent changes in membrane conductance to Na+ and K+ explain the action potential?
how can hodgkin and huxley go about answering their question: Could voltage-dependent changes in membrane conductance to Na+ and K+ explain the action potential?
: Construct a model that accurately describes all the relevant variables and their mathematical relations to one another and show that the features of the action potential follow from that
aka assume it does and use the math that would be required in this assumption and see if it follow (i think)
findings of Hodgkin and Huxley on whether voltage-dependnent changes in membrane conductance to Na+ and K+ explains action potential
Hodgkin and Huxley explain action potentials in terms of known conductance changes.
But they had only a phenomenal model of the conductance changes.
They can model this magic; but they can’t explain HOW it works.
sympathetic nervous systems
active in emergency situations
dilates pupils makes heart beat stronger and faster relaxes airways letting you breathe more deeply inhibits digestion increases blood flow to skeletal muscles
in emergency situations you ___ nervous system is active
when your body is at rest your ___ nervous system is active
sympathetic nervous system when in emergency situations
parasympathetic nervous system when body is at rest
parasympathetic nervous system
active when body is at rest
it constricts pupils makes heart beat more slowly constricts airways stimulates digestion reduces blood flow to skeletal muscles
quantal release
communication between neurons is made up of quantum packets
packets of neurotransmitters
vesicles go to wall of neuron
each quantum gives the same post synaptic response (quantal amplitude)
one vesicle=one quanta
m=np (this means that the mean quantal content=number of vesicles/quanta multiplied by the probability of release)
freeze fracture technique
neuromuscular junction is stimulated
this causes it to relase stuff
freeze at this point
then fracture it
this gives you the number of vesicles fusing at that instance and the number of quanta released
when graphed you get a 1:1 line of vesicles fused and quanta released
main categories of neurotransmitters
acetylcholine (most active at the neuromuscular junction)
biogenic amines
Amino acids
neuropeptides