MIDTERM: Mycobacteria Flashcards by Jannine Mikaela

Classify the Runyon group: M.thermoresistible

Scotochromogens

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measures the presence of a lipase.  Hydrolysis causes a pink color change

Tween 80 hydrolysis

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Exposed to direct sunlight, MTB for culture are killed within

within 2 hours

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grow on chocolate agar and appear as smooth. opaque colonies resembling staphylococci or yeast.

M. chelonae

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Contains 0.1% enzymatic hydrolysate of casein, which improves recovery of isoniazid-resistant Mycobacterium tuberculosis

Middlebrook 7H11

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positive control organism for urease

M. fortuitum

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Test that utilize M.intracellulare as negative control

Niacin,Nitrate, Tween 80, Arylsulfatase (Naya and Nit twerk around Ariel during INTRAmurals)

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does not reduce nitrates or assimilate iron, but it is suceptible to polymyxin B and resistant to ciprofloxacin.

M. chelonae

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have the ability to grow at 45°C; does not grow well on LJ agar. It appears as a film after 3 to
4 weeks of incubation

M. avium

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Classify the Runyon group: M.smegmatis

Rapid growers

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Supplemented with cycloheximide,
lincomycin, and nalidixic acid

L-J Mycobactosel

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Not pigmented unless exposed to light optimally during their early growth

Photochromogen

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span of growth in liquid media

10 days

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appear as a “sun-spot” with starlike or asteroid margins; rough colony has a “lacy” characteristic

M. avium

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Test that utilize M.gordonae as negative control

5% NaCl (Chicken GORDON with salt(NaCl)

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Pigmented when grown in the dark or light

Scotochromogen

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Battey Bacillus
 In AIDS patients

MYCOBACTERIUM AVIUM COMPLEX

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Negative result reaction in Niacin Test

M. intracellulare-No color change (milky white)

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a disseminated infection with multiple organ
involvement.

Miliary TB

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Decontaminating agent of MYCOBACTERIUM LEPRAE

N acetyl-L-cysteine(NALC)

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MYCOBACTERIUM TUBERCULOSIS measurement

0.2-0.6 micron in diameter and 1-4 micra in length

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Test that utilize M.tuberculosis as negative control

68C catalase, Tellurite, TCH (TCH & Tell got 68 on TB test)

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liquid media that is used as the standard for Isolating mycobacteria

Löwenstein Jensen (L-J)

M.xenopi runyon's class

Scotochromogen

a widely used medium for the isolation and antimicrobial susceptibility testing of mycobacteria

Middlebrook 7H11 agar

Droplets of dried sputum in the air -

8-10 days

3-week-old colony growing on Middlebrook 7H11agar shows a dark, dense center and a wrinkled periphery,

Colony of M. kansasii

pyrazinamide is converted into pyrazinoic acid through the action of the pyrazinamidase enzyme

Pyrazinamide test

Not pigmented when grown in the dark or in light

Nonchromagen

have a strong yellow color; a positive catalase reaction, reduction of nitrate to nitrite, and rapid hydrolysis of Tween 80

M. kansasii on Middlebrook 7H11 agar.

detected by adding phenolphthalein to thec olony/ substrate mixture and observing the formation of a pink color

Arylsulfatase test

Span of MTB In sputum -

20-30 hours

disseminated disease in HIV-infected patients

MYCOBACTERIUM GENAVENSE

etiologic agent of leprosy, also called Hansen's disease

MYCOBACTERIUM LEPRAE

rough, buffered colonies that appeared within 3 weeks

M.tuberculosis in LJ agar

Mycobacteria that are thermolabile in catalase test

M. tuberculosis and M. bovis

Disinfection of sputum -

24 hours in 5% phenol

Positive reaction for catalase test

Bubbles

positive control organism for Tellurite test

M.avium

Optimum temperature for M. fortuitum cultured in 7H11 & LJ

43 deg. Celsius

Contains 2% glycerol, which enhances the growth of Mycobacterium avium complex (MAC)

Middlebrook

Highly resistant to drying (protected from sunlight)

MYCOBACTERIUM TUBERCULOSIS

Bairnsdale ulcer

MYCOBACTERIUM ULCERANS

MTB antigen.

purified protein derivative (PPD)

positive control organism for TCH

M.bovies

Growth at 28°C on an egg based medium containing 5% NaCl

NaCl tolerance test

not culturable; unique manifestation in a way that they infect the nerves instead the cells

MYCOBACTERIUM LEPRAE

a Runyon group II scotochromogens, characterized by yellow-orange pigment

M. gordonae

Reagent strip impregnated with substrate cyanogen halide = yellow color

Niacin accumulation tests

Classify the Runyon group: M.terrae-triviale

Non-photochromogens

Nontuberculous mycobacteria pigment

Runyon's classification

Supplemented with penicillin and nalidixic acid

L-J Gruft

Duration of MTB in Dried sputum

6-8 months

Colonies appear as cream-colored, dry, and wrinkled.

M. tuberculosis on Middlebrook 7H11 agar

colonies appear dark and dense with smooth edges and a somewhat lighter center

M. chelonae

digesting agent MYCOBACTERIUM LEPRAE

2-4% NaOH

The only mycobacteria that is negative to NaCl Tolerance test

M.chelonae

Tap-water bacillus

MYCOBACTERIUM GORDONAE

M.kansassi runyon's classification

Photochromogen

Contains twice the concentration of malachite than LöwensteinJensen green

Petragnani medium

growth requirement for hemoglobinor hemin

MYCOBACTERIUM HAEMOPHILUM

contains casein hydrolysates that improve the recoveryof isoniazid-resistant strains of M. tuberculosis and shortens the incubation time for M. avium complex isolates.

Middlebrook 7H11 agar

is miliary tuberculosis in the bones or spine

Pott's disease

Supplemented with carbenicillin (for inhibition of pseudomonads), polymyxin B, trimethoprim lactate, and amphotericin B

Middlebrook 7H10

positive for Arylsulfatase test

M. fortuitum and M. abcesuss;

chronic granulomatous infections involving skin and soft tissue, often called"swimming pool granuloma" or "fish tank granuloma"

MYCOBACTERIUM MARINUM

Commonly used medium; good recovery of M. tuberculosis but poor recovery of many other species; M. genovense fails to grow

Löwenstein Jensen (L-J)

positive control organism for Tween 80 hydrolysis

M.kansasii

used to detect MTB-infected individuals

Tuberculin skin test

can also grow on modifiedMac-Conkey agar, without crystal violet, at 37°C;

M. fortuitum

"yellow bacillus,

MYCOBACTERIUM KANSASII

colonies have a rough appearance and exhibit serpentine cording,exemplified by the darker areas.

M. tuberculosis

positive control organism for Niacin test

M.tuberculosis

Supplemented with hemin, hemoglobin, or ferric ammonium citrate; increases recovery of Mycobacterium haemophilum

Heme-supplemented media (egg or agar based)

Base on the ability to convert ferric ammonium citrate to an iron oxide

Iron uptake test

may appear as weakly staining. beaded, gram-positive bacilli, suggesting the possibility of a rapidly growing mycobacterium.

M. chelonae