Misc Body Fluids

Question 1

True or False:
There is no definitive test to differentiate menstrual blood from venous blood.

Answer 1

True

Question 2

When radial gel diffusion is used for the identification of urine, it can be both
________
and
________
in nature when one takes into account both the color change and the diameter of the reaction circle generated.

Answer 2

qualitative, quantitative

Question 3

Which urine test Heats creatinine and sodium nitroprusside with potassium ferricyanide as an oxidizing agent to form a blue product?

Answer 3

Salkowski test

Question 4

Which urine test will form a blue precipitate

Answer 4

Radial gel diffusion with BTB and Urease

Question 5

In which urine test urea will turn pink/red

Answer 5

DMAC

Question 6

What urine test has Creatinine Reacts with picric acid in a solution of toluene or benzene (alkaline environment) and Forms a bright orange/red Creatinine Picrate product

Answer 6

Jaffe test

Question 7

What urine test Uses an alkaline solution of K2HgI4. Results in a pale solution that becomes deeper yellow (or brown) in the presence of ammonia

Answer 7

Nessler’s Reagent

Question 8

True or False:
Urea, the second largest constituent of urine after water, is specific to urine. Therefore detection/identification of urea in a forensic unknown is confirmation of the presence of urine.

Answer 8

False

Question 9

What vaginal secretion test has Cells without glycogen stain golden yellow
and cells with glycogen stain chocolate brown

Answer 9

Lugol’s Iodine

Question 10

What vaginal secretion test has Buccal cells stain red, Vaginal cells stain orange, and Skin cells stain magenta

Answer 10

Keratin staining

Question 11

What vaginal secretion test has Cells without glycogen stain magenta

Answer 11

Periodic Acid Schiff (PAS)/Alcian Blue

Question 12

In the first phase of the Edelman assay for the identification of feces, urobilinogen is oxidized with
_________ to produce urobilin (a pink-red product). In the second phase of the Edelman assay for the identification of feces, urobilin reacts with
_______
to produce a complex that fluoresces apple-green under UV light.

Answer 12

Mercury chloride, zinc chloride

Question 13

True or False:
The term “Vaginal Secretions” is biologically correct.

Answer 13

False

Question 14

Most common chemical tests for the identification of urine involve the detection of
________
and
_______
, both of which are components of urine that _____
unique to urine, but they _____
unique in their combination

Answer 14

urea, creatinine, are not, are

Question 15

True or False:
Microscopy is the best identification technique for feces, as hairs and undigested food residues may be readily identifiable.

Answer 15

True

Question 16

True or False:
It is relatively easy to obtain a full, human DNA profile of a fecal material donor due to the abundance of eukaryotic cellular material found in most feces.

Answer 16

False

Question 17

Immunoassays, such as ELISA and RSID-Urine, use the protein _____________________________ as the basis for the identification of urine.

Answer 17

THG

Question 18

True or False:
In normal healthy individuals urine is an abundant source of cellular material, perfect for DNA profiling.

Answer 18

False

Question 19

List at least two characteristics or components of vomitus that can aid in its identification.

Answer 19

Vomitus has a low pH due to the stomach acid content and a lot of salivary alpha amylase due to the constant swallowing of saliva, which can both be used for identification of vomit.

Question 20

True or False:
Urine stains can be visualized under the ALS, but they can be hard to locate because stains tend to more diffuse and fluoresce less than other body fluids.

Answer 20

True

Question 21

True or False:
Recognition of the characteristic ammonia odor of urine when processing a crime scene or an item of evidence should be documented and can serve as one presumptive method of identification.

Answer 21

True