Miscellaneous GNB-Coccobacilli Exam 3 Flashcards Preview

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1

Vibrio general info.

Oxidase pos. Gram neg bacilli. Ferments glucose. MAC pos. Non-lactose fermenter except for V. vulnificus. Facultative anaerobes. likes h20 environment. NOT apart of normal flora.

2

Aeromonas general info

Oxidase pos. Gram neg bacilli. Ferments glucose. MAC pos. Non-lactose fermenter except for V. vulnificus. Facultative anaerobes. likes h20 environment. NOT apart of normal flora. Produces deoxyribonuclease.

3

Chromobacterium general info.

Oxidase pos. Gram neg bacilli. Ferments glucose. MAC pos. Non-lactose fermenter except for V. vulnificus. Facultative anaerobes. likes h20 environment. NOT apart of normal flora.

4

What is the specimen for vibrio, aeromonas ,and chromobacterium?

Stool or rectal swabs (stool needs to be transported in Cary-Blair medium when vibrio is suspected.

5

What is the selected media for Vibrio spp?

Thiosulfate citrate bile salts sucrose (TCBS). Bile salts inhibit gram pos organisms and high ph (8.6) inhibits other intestinal flora.

6

Presumptive ID for Vibrio?

Medium to large, smooth, opaque colonies with greenish hue on SBAP.

7

Presumptive ID for Aeromonas?

Large, round, raised, and opaque.

8

Chromobacterium- Presumptive ID?

round, smooth, convex, black to dark purple with almond like smell on SBAP.

9

Should oxidase testing be done on MAC plate?

No. Fermentation of lactose can create acids that can acidify the medium and if falls below pH of 5.1 can get false negatives.

10

What organism is the cause of cholera?

Vibrio cholerae

11

How is cholera mostly transmitted?

Through contaminated seafood ( waters exposed to feces)

12

What vibrio serotypes have been involved in epidemics/pandemics?

01 and 0139

13

What virulence factors does Vibrio cholerae have?

Cholera toxin (CT), hemolysins, cytotoxins, and pili.

14

What does cholera lead to?

dramatic fluid loss (dehydration), hypotension-damage to kidneys, and death can occur. *Rice water stools* Blood is absent in stool.

15

What is the treatment for cholera?

Two vaccines OUTSIDE US. Antibiotics decrease severity (Tetracycline and doxycycline). Rehydration is vital.

16

What kind of specimens are for Vibrio vulnificus?

Wounds, blood, or stool (from ingestion of seafood) *oysters* Doesn't spread from person to person.

17

How do you treat Vibrio vulnificus?

Amputation of infected limbs perhaps. Toxins released include lipases, proteases, and collagenases. *Doxycycline plus ceftazidime recommended.

18

Signs of Vibrio parahemolyticus?

watery diarrhea, cramping, nausea, vomiting, fever w/in 24 hours. Self-limiting to 3 days. *All cases of Vibrio spp. must be reported to state health depts.*

19

What is the treatment for Vibrio parahaemolyticus?

Hydration. Usually no antibiotics (tetracycline in severe cases)

20

What organism can cause gastroenteritis with diarrhea most commonly in children?

Aeromonas spp. (Aeromonas hydrophilia)

21

What can Aeromonas spp. cause?

HUS with kidney failure

22

What organism starts as cellulitis and then progresses to lymphadenitis to systemic infection possible with visceral abscess formation and shock?

Chromobacterium violaceum

23

What is the treatment for Chromobacterium violaceum?

No set guidelines. Pencillin activity is variable. *Cefotaxime is effective*

24

Media for Bartonella?

Fresh chocolate *NO BAP and MAC*

25

Media for Streptobacillus moniliformis?

Sheep in increased CO2 and humidity.

26

Media for Helicobacter?

Chocolate or Brucella agar

27

Campylobacter media?

CAMPY-BAP, CAMPY CVA, and CCDA (modified charcoal cefoperazone deoxycholate agar

28

Media for Brucella?

Brucella agar (adds heated horse serum)

29

Media for Francisella tularensis?

Media with cysteine (buffered charcoal yeast extract agar)

30

What causes Tularemia (disease with a fever of 104 degrees C)?

Francisella tularensis