Mixing Studies

Question 1

This test is used to identify specific factor deficiency by mixing correction reagents with the patient’s plasma and then performing the PT and/or APTT.

Answer 1

MIXING STUDIES/ SUBSTITUTION STUDIES

Question 2

It is also used to detect lupus anticoagulants and specific factor inhibitors.

Effects of these anticoagulants to the clotting time is PROLONGED

Answer 2

MIXING STUDIES/ SUBSTITUTION STUDIES

Question 3

Mixing studies are laboratory tests performed on blood plasma to differentiate between two main causes of abnormal clotting times:

Answer 3

Factor deficiency
Presence of inhibitors

Question 4

•: When specific clotting factors (proteins) are present at low levels in the blood.

•: Substances in the blood that interfere with the clotting process.

Answer 4

Factor deficiency

Presence of inhibitors

Question 5

INTRINSIC COAGULATION
PATHWAY FACTORS

Answer 5

XII, XI, IX, VIII
(12,11,9,8)

Question 6

EXTRINSIC COAGULATION
PATHWAY FACTORS

Answer 6

(Ill), VII
(3), 7

Question 7

COMMON COAGULATION
PATHWAY FACTORS

Answer 7

X, V, II, 1 (10,5,2,1)

Question 8

Factor____ doesn’t have deficiency because it is always found in our tissues

Answer 8

3

Question 9

FIBRINOGEN GROUP

Present in PLASMA
Absent in SERUM

Answer 9

I, V, VIII, XIII
(1, 5, 8, 13)

Question 10

PROTHROMBIN GROUP

Answer 10

II, VII, IX, X (2,7, 9, 10)

Question 11

CONTACT GROUP

All present in SERUM

Answer 11

XII, XI, PK, HMWK

(12, 11, Prekallikrein, High molecular weight kininogen)

Question 12

ADSORBED PLASMA

Adsorbed using____ or _____

Answer 12

aluminum hydroxide or barium sulfate

Question 13

PROCEDURE: BARIUM SULFATE ADSORPTION
1. 1 mL plasma +______. Mix well.

2. Incubate, 37°C for___ mins with frequent mixing
3. Centrifuge, 2000 rpm
4. Collect the supernatant =_____

Answer 13

50 mg barium sulfate

15mins

Adsorbed Plasma

Question 14

PROCEDURE: BARIUM SULFATE ADSORPTION
1. 1 mL plasma +______. Mix well.

2. Incubate, 37°C for___ mins with frequent mixing
3. Centrifuge, 2000 rpm
4. Collect the supernatant =_____

Answer 14

50 mg barium sulfate

15mins

Adsorbed Plasma

Question 14

PROCEDURE: ALUMINUM HYDROXIDE ADSORPTION
1. Aluminum Hydroxide suspension preparation (_______ + ________)

2. 1 part aluminum hydroxide suspension +9 parts _____. Mix well.
3. Incubate, 37°C for____ mins
4. Centrifuge, 2500 rpm
5. Collect the supernatant fluid =____

Answer 14

1 g aluminum hydroxide + 4 mL
distilled water

9 parts citrated plasma

5mins

Adsorbed Plasma

Question 15

AGED SERUM

PROCEDURE:*
1. Allow whole blood to clot at 37°C for_____

2. 1 part _____ + 9 parts ______(in the previous procedure).
3. Incubate for additional____ at 37°C
4. Centrifuge, 10 minutes. Collect the serum
5. Prepare____ dilution of the serum with_____ before testing

Answer 15

3 hrs

0.1 M sodium citrate + whole blood

2 hours

1:5

0.85% sodium chloride

Question 16

In AGED PLASMA

What are absent?

Answer 16

Factor V and XIII = Labile Factor (easily destroyed)

Question 17

In ADSORBED PLASMA

What are absent??

Answer 17

Factor II, VII, IX and X = Easily Adsorbed

9, 10, 7, 2

Question 18

In AGED SERUM

What are absent?

Answer 18

Factor I, II, V, VIII and XIII =Consumed because they are clotted

1, 2, 5, 8, 13