TEST FOR FIBRINOLYSIS Flashcards
(16 cards)
TEST FOR FIBRINOLYSIS
- Whole blood clot lysis time (WBCLT)
- Euglobulin Clot Lysis Time
- Protamine Sulfate Test
- Ethanol Gelation Test
- Latex D-Dimer Test
• Clot should remain intact for approximately 48 hours after 37°C
• Clot lysis of dissolution prior to 48 hours is indicative of excessive primary fibrinolysis.
Whole Blood Clot Lysis Time (WBCLT)
Whole Blood Clot Lysis Time (WBCLT)
• Clot should remain intact for approximately____ hours after 37°C
• Clot lysis of dissolution prior to 48 hours is indicative of_____
48 hrs
excessive primary fibrinolysis.
•________: are protein that precipitates when plasma is diluted with water and acidified.
It consists of (4)
EUGLÖBULIN
plasminogen, plasmin, fibrinogen and fibrinogen activators
Euglobulin clot lysis time
_____+____= to precipitate euglobulin
Euglobulin dissolved in____+____ = TO CLOET EUGLOBULIN
Plasma + acetic acid
buffer + thrombin
Whole Blood Clot Lysis
• Clot lysis in ______= NORMAL
• Clot lysis_____ = INCREASED FIBRINOLYSIS
48 hours
< 48 hours
Euglobulin Clot Lysis Time
•________ =NORMAL
•________: INCREASED FIBRINOLYSIS
No clot lysis > 2 hours
<2 Hours of Lysis
Detects the presence of FIBRINOGEN MONOMERS
Protamine Sulfate test
Protamine Sulfate Test
PRINCIPLE:
______ +______= PARACOAGULATION (gel-like clot)
Plasma + Protime Sulfate
Less sensitive test but more specific test than protamine sulfate
Detects the presence of fibrinogen Monomers
Ethanol Gelation Test
Ethanol gelation test
Reagents: PEN
Plasma
Ethanol
NaOH
Protamine sulfate test
Ethanol Gelation Test
NORMAL:______
ABNORMAL:______
No gel formation
Gel formation
To monitor if the fibrinolytic pathway is functional
Latex D-Dimer Test
Latex D-Dimer Test
Used to evaluate specific fragments (D-dimer);
When_____ is used up, D-dimer is present
fibrinogen
Latex D-Dimer Test
PRINCIPLE: based on______
The test uses latex bead coated with monoclonal antibody specific for D-dimer (Only) but not to fibrinogen and FDP
Enzyme Immunoassay
D Dimer Test
The test has no specificity for fibrinogen and therefore identifies____ action
plasmin
