MLSCI340 ANAEROBES

Question 1

Test results for ID of Actinomyces israelii

Answer 1

col morph= molar toorh
%= branching gpb
cat= neg

Question 2

Test results for ID of Bacteroides fragilis

Answer 2

%= gncb
cat= pos
BBEA= pos
KVC= RRR

BBEA= Bacteroides Bile Esculin Agar

Question 3

How is susceptibility performed for anaerobic organisms ?

Answer 3

• E-test (modified KB)
• microbroth dilution

Question 4

Principle of reverse CAMP. What is a positive result ?

Answer 4

Principle:

• STB (S. agal) is streaked down the centre of BAP*
• Test organisms are inoculated at a right angle

Positive: C. perfringens will produce an open arrowhead (>) = synergistic hemolysis

Question 5

Test results for ID of Peptostreptococcus

Answer 5

%= gpc ch
cat= neg
KVC= RSR

Question 6

Test results for ID of Cutibacterium acnes

Answer 6

%= gpb pleo
cat= pos
KVC= SSR

Question 7

Test results for ID of C. perfringens

Answer 7

col morph= dzh
%= gpb boxcars
cat= neg
reverse CAMP = pos
lecithinase= pos

Question 8

Test results for ID of C. sporogenes

Answer 8

col morph= rhz
%= gpb spores
cat= neg
reverse CAMP= neg
lipase= pos