mock exam Qs /- JJ Flashcards
(22 cards)
Out of the features of a plasmid, which one allows for the selection of bacterial clones that have incorporated cloned DNA?
The antibiotic resistant gene
Bacteria such as E. coli is used for expression of cloned DNA bc:
They grow v fast
Inexpensive culture conditions
Allow protein production in high yields
Many molecular tools available
You know that mutation triggers the expression of a gene. Which of the following techniques can you use to identify the gene affected?
Northern blot and in situ hybridisation
What is the main goal of microarray experiment?
To investigate the expression of thousands of genes at the same time
Phenotype is:
the observable traits of an individual
Prince Leopold suffered haemophilia because:
Queen victoria was a carrier
Which RNA polymerase subunit is not part of the processive core and gets released after initiation?
Sigma
Which sigma factor will not bind to -10 and -35 region?
sigma 54
2 component regulatory systems involve
A histidine kinase that phosphorylates a regulator when an inducer is present
the Lac operon:
responds to presence of lactose using a respressor and glucose using an activator that binds to cAMP
When checking the resulting products of PCR by gel electrophoresis
shorter fragments will migrate faster
Different restriction enzymes
Recognise specific sequences and produce ends that are occasionally compatible
DNA ligases are responsible for
the formation of phosphodiester bonds between DNA fragments
What steps are part of cloning bacteria?
DNA amplification
DNA digestion
DNA ligation
Electroporation
A plasmid that is transfected into animal cells and does not contain an origin of replication but carries a selection marker may lead to…
stable integration in the chromosome and spread in the population in the presence of a selection marker
Northern blot allows
To determine the presence and size of a transcript
How would you identify the cells expressing a particular gene in the development of the endoderm of D. melanogaster?
In situ hybridisation
Protein fusions allow studying
gene expression thanks to the presence of a reporter
the most probable outcome of the cross of two heterozygous individuals carrying a dominant mutation is:
75% will have mutant genotype and phenotype
Outcome of two heterozygous individuals each carrying a recessive mutation is
75% mutant genotype
25% mutant phenotype
mutations are always
changes in DNA sequence
minimal requirement to be affected with Huntingtons disease is:
mother is a carrier
