Mod 1 Flashcards

(52 cards)

1
Q

Cause of false POS or NEG in Rh typing:

- Failure to add reagent

A

False neg

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2
Q

Cause of false POS or NEG in Rh typing:

- Over shaking

A

False neg

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3
Q

Cause of false POS or NEG in Rh typing:

- Rouleaux

A

False Pos

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4
Q

Cause of false POS or NEG in Rh typing:

- Reagent deterioration

A

Fase neg

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5
Q

Cause of false POS or NEG in Rh typing:

- Cold autagglutinins

A

False Pos

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6
Q

Cause of false POS or NEG in Rh typing:

- using wrong antiserum

A

False pos OR false neg

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7
Q

Cause of false POS or NEG in Rh typing:

- Positive DAT on cells

A

False pos

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8
Q

Cause of false POS or NEG in Rh typing:

- incorrect cell suspension

A

False Neg

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9
Q

Cause of false POS or NEG in Rh typing:

- Warm autoagglutinins

A

False pos

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10
Q

Cause of false POS or NEG in Rh typing:

- Polyagglutinable cells

A

False Pos

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11
Q

Cause of false POS or NEG in Rh typing:

- blocking of antigen sites (HDFN)

A

False neg

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12
Q

Cause of false POS or NEG in Rh typing:

- Incorrect antiserum-to-cell ratio

A

False Neg

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13
Q

Cause of false POS or NEG in Rh typing:

- using a post-transfusion sample of an Rh = patient who received Rh + blood

A

False Pos

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14
Q

Cause of false POS or NEG in Rh typing:

- contaminated reagent `

A

False Pos OR False Neg

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15
Q

””"”Cause of false POS or NEG in AGT typing:

- over centrifugation or over reading

A

False Pos

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16
Q

””"”Cause of false POS or NEG in AGT typing:

- too short incubation

A

False neg

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17
Q

””"”Cause of false POS or NEG in AGT typing:

- improper storage of cells, serum, reagent

A

False neg

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18
Q

””"”Cause of false POS or NEG in AGT typing:

- Dirty glassware

A

False pos

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19
Q

””"”Cause of false POS or NEG in AGT typing:

- Test cells have positive DAT

A

False pos

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20
Q

””"”Cause of false POS or NEG in AGT typing:

- Omission of AHG

A

False neg

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21
Q

””"”Cause of false POS or NEG in AGT typing:

- bacterial contamination

22
Q

””"”Cause of false POS or NEG in AGT typing:

- Contaminated AHG

23
Q

””"”Cause of false POS or NEG in AGT typing:

- centrifugation over or under

24
Q

””"”Cause of false POS or NEG in AGT typing:

- improper washing

25
""""Cause of false POS or NEG in AGT typing: | - Contamination
False neg
26
""""Cause of false POS or NEG in AGT typing: | - Elution of antibody
False neg
27
""""Cause of false POS or NEG in AGT typing: | - Temperature
False neg
28
""""Cause of false POS or NEG in AGT typing: | - weak/heavy cell suspension
False neg
29
O: percent and sugar
45%, fucose
30
A: percent and sugar
40%, N-acetylgalactose amine
31
B: percent and sugar
11%, D-galactose
32
AB: percent and sugars
4%, (all three sugars present)
33
Rh antigens: percent and Weiner | - D
85%, Rho
34
Rh antigens: percent and Weiner | - C
70%, rh'
35
Rh antigens: percent and Weiner | - E
30%, rh''
36
Rh antigens: percent and Weiner | - c
80%, hr'
37
Rh antigens: percent and Weiner | - e
98%, hr''
38
DISCREPANCIES: extra in the forward
1. Rouleaux 2. Cold Auto Antibodies 3. Aquired B-like antigen 4. Polyagglutination
39
DISCREPANCIES: Mixed field in the forward
1. Recent transfusion with 'out-of-group' blood 2. Bone marrow transplant 3. Exchange transfusions 4. Fetal-maternal Bleeding 5. A3 or B3 subgroups 6. Chimeras
40
DISCREPANCIES: Weak/Missing in the forward
1. Subgroups of A or B
41
DISCREPANCIES: Weak/missing in the reverse
1. Age of patient 2. Disease state 3. True Chimera twins
42
DISCREPANCIES: Extra in the reverse
1. Rouleaux 2. Auto Abs 3. Allo Abs
43
Resolution for Rouleaux in the forward
Wash cells 4-6 times
44
Resolution for Cold auto abs in the forward
Warm saline wash 4-6 times
45
Resolution for Acquired B-like Ags
Check patient diagnosis for GI tract disease
46
Resolution for Polyagglutination in the forward
Use monoclonal reagents
47
Resolution for mixed field reactions in the forward
Check patient history
48
Resolution for subgroups of A or B in the forward
- test with A1 lectin (will be + if A1) - test with anti-a,b (will be + if Ax or Bx) - Secretor studies
49
Resolutions of Weak/missing reactions in the reverse
increase serum to cell ratio - incubat at RT - Incubate at 4 degrees - Use enzyme treated reverse cells
50
Resolution to rouleaux in reverse
saline replacement technique
51
Resolution to auto Abs in reverse
Pre-warm testing
52
Resolution to Allo Abs in reverse
- Prove a subgroup with 3+3 rule | - antibody screen