mod 1-5 Flashcards
(103 cards)
1
Q
Applications where the product is not hazardous, but must be kept contaminant free. Provides HEPA filtered supply to the work surface and a particulate-free work area.
A
Laminar Flow
Clean Bench
2
Q
In a front, rear and top through
HEPA Filter
A
CLASS I (Open front)
3
Q
70% Recirculated through HEPA; exhaust through HEPA
A
CLASS II Type A
4
Q
30% Recirculated through HEPA; exhaust via HEPA and hard-ducted
A
CLASS II Type B1
5
Q
No recirculation, total exhaust via HEPA and hard-ducted
A
CLASS II Type B2
6
Q
Same as CLASS II-A, but plenums under negative pressure to room and exhaust air is ducted.
A
CLASS II Type B3
7
Q
Supply air inlets through 2 HEPA Filters
A
CLASS III
8
Q
BSC
A
biological safety cabinet
9
Q
HEPA
A
high-efficiency particulate air
10
Q
Lfm
A
linear feet per minute
11
Q
ordinary combustible solid materials, such as paper, wood, plastic, and fabric
A
Class A
12
Q
flammable liquids/gases and combustible petroleum products
A
Class B
13
Q
energized electrical equipment
A
Class C
14
Q
combustible/reactive metals, such as magnesium, sodium, and
potassium
A
Class D
15
Q
Rescue anyone in immediate danger
A
RESCUE
16
Q
Activate the institutional fire alarm system
A
ALARM
17
Q
Close all doors to potentially affected areas
A
CONTAIN
18
Q
Attempt to extinguish the fire, if possible, (exit the area)
A
EXTINGUISH
19
Q
A detector converts the electromagnetic radiation (light energy) transmitted by a solution into an electrical signal.
A
Photodetectors
20
Q
The more light transmitted, the _ energy, and the _ the electrical signal that is measured.
A
The more light transmitted, the more energy, and the greater the electrical signal that is measured.
21
Q
It is the simplest detector; least expensive; temperature sensitive. composed of selenium on a plate of iron covered with transparent layer of silver
A
BARRIER LAYER CELL/ PHOTOCELL/ PHOTOVOLTAIC CELL
22
Q
most commonly used detector – measures visible and UV regions. It has excellent sensitivity and has a rapid response – detects very low levels of light
A
PHOTOMULTIPLIER TUBE
22
Q
contains cathode and anode enclosed in a glass case
A
PHOTOTUBE
23
Q
not as sensitive as PMT but with excellent linearity. measures light at a multitude of wavelengths – detects less amount of light.
A
PHOTODIODE
24
Electrical energy from a detector is displayed on some type of digital display or readout system.
Readout devices
25
states that the concentration of the unknown substance is _ to the absorbed light (absorbance or optical density) and _ to the amount of transmitted light/reflected light (% Transmittance)
states that the concentration of the unknown substance is directly proportional to the absorbed light (absorbance or optical density) and inversely proportional to the amount of transmitted light/reflected light (% Transmittance)
26
states that the concentration of the unknown substance is directly proportional to the absorbed light (absorbance or optical density) and inversely proportional to the amount of transmitted light/reflected light (% Transmittance)
Beer’s Law
27
Is the amount of light absorbed. Proportional to the inverse log of transmittance.
ABSORBANCE
28
Is the ratio of the radiant energy transmitted (T) divided by the radiant energy incident on the sample (I).
PERCENT TRANSMITTANCE
29
Means the blank contains serum but without the reagent to complete the assay.
BLANKING TECHNIQUE
30
corrects absorbance caused by the color of the reagents.
Reagent blank
31
measures absorbance of the sample and reagent in the absence of endproduct, and corrects the measurement for optical interference (like hgb) absorbing the wavelength of measurement.
Sample blank
32
-heating and sterilization
-most commonly used
-high degree of thermal resistance
-free from the magnesium lime – zinc group of elements, heavy metals, arsenic and antimony.
-515 degree Celsius (Pyrex)
Borosilicate glass ( pyrex and kimax)
33
-high resistance to alkali
-its thermal resistance is less as compared to borosilicate glass
Boron-free glassware/soft ware
34
-special alumina-silicate glass
-6x stronger than borosilicate
Corex (Corning)
35
-utilized for high thermal
-drastic heat shock and extreme chemical treatment with acids (except hydrofluoric acid) and dilute alkali
-can be heated up to 900 degree Celsius
-utilized for extreme chemical treatment with acids and dilute alkali
Vycor (Corning)
36
-made up of soda-lime glass and a mixture of calcium, silicon and sodium oxides.
-has a poor resistance to high temperature – easy to melt and used to make disposable glasswares.
Flint glass
37
great for general storage and transport
Polystyrene
38
used in making other plastics much often than it is used in its pure form
Polyethylene
39
commonly used to manufacture plastic laboratory supplies
Polypropylene
40
used in machines where reagents and wastes pass through
Tygon
41
solid polymer that is considered as one of the world’s most slippery substance
Teflon
42
it delivers the exact amount it holds into a container
TO DELIVER (TD)
43
it holds the particular volume but does not dispense the exact volume.
TO CONTAIN (TC)
44
it has a continuous etched rings on top of the pipet; exact volume is obtained when the last drop is blown out.
Blow out
45
absence of etched rings; liquid is allowed to drain by gravity.
Self-draining
46
Volumetric Pipet
Ostwald Folin
Pasteur Pipet
Automatic Macro or Micropipettes
Transfer Pipet
47
Serological Pipet -
Mohr Pipet
Bacteriologic Pipette
Ball, Kolmer, Khan Pipette
Micropipettes
Graduated or Measuring Pipet
48
Sahli-Hellige Pipette
Lang-Levy Pipette
RBC and WBC Pipettes
Kirk and Overflow Pipette
Micropipettes (<1mL) - TC Pipettes
49
for non-viscous fluid; self-draining; small amount left in the tip should not be blown out.
Volumetric pipet
50
for viscous fluid; with etched ring
Ostwald folin
51
transfer fluid without consideration of a specific volume.
Pasteur pipet
52
It relies on a piston for suction creation to draw the sample into a disposable tip.
Air Displacement Pipette
53
It operated by moving the piston in the pipette
tip or barrel, much like a hypodermic syringe.
Positive Displacement Pipette
54
It obtains liquid from a common
reservoir and dispenses it repeatedly.
Dispenser/ Diluter Pipette
55
with graduations to the tip; blowout pipet.
Serologic pipet
56
without graduations to the tip, calibrated between 2 marks; self-draining pipet.
Mohr pipet
57
Distilled water is the for
TD pipettes.
58
The first consideration in the measurement of blood analyte is
specimen collection
58
Mercury for
TC pipettes.
59
a commonly used sample in most Clinical Chemistry Analyses.
Venous blood
60
its less hyperosmolar effect on blood cells compared to K3 EDTA.
K2 EDTA
61
recommend the use of K2 EDTA due to its less hyperosmolareffect on blood cells compared to K3 EDTA.
HYPEROSMOLAR
62
Green bottle for blood culture bottle
aerobic
63
Orange bottle for blood culture bottle
anaerobic
64
yellow bottle for blood culture bottle
pediatric
65
use of blood culture bottle and number of inversion
8-1, Pulls out calcium that complement from acting and being initiated in the sample. Slows phagocytosis and reduce antibiotic effects.
66
SPS( soium polyethanol sulfonate )
yellow(sterile) additive
67
Used for blood culture system Recover microorganisms causing blood infections
yellow tube
68
light blue additive
sodium citrate
69
This is use for coagulation studies such as PT/INR, PTT, Anti-Xa, fibrinogen, D- dimer and TEG studies
blue tube
70
For ESR studies only. Used for Westergren Sedimentation Rate
black
71
enhances coagulation to separate serum
yellow/red top (plastic), red top glass)
72
4:1 buffered sodium citrate
black
73
clot activator, polymer gel,SST
gold
74
Separate cells from the serum of the blood sample.
gold
75
This tube is widely used for ammonium levels, pH level analysis, Blood Gas Analysis
green
76
Most common additive is EDTA which is used for many tests such as CBC and coagulation with factor V Leiden. It also preserves cellular morphology and inhibit platelet clumping
lavender
77
Average human body contains approximately _ quarts ( _ L) of whole blood.
Average human body contains approximately 5 quarts (4.73 L) of whole blood.
78
approximately (5- 6L) of whole blood
approximately (5-6 L) of whole blood for male
79
approximately (_-_ L) of whole blood
approximately (4-5 L) of whole blood for female
80
WHOLE BLOOD comosped of :
_% PLASMA – _ L
_ CELLS – _ L
WHOLE BLOOD comosped of :
60% PLASMA – 2.84 L
40% CELLS – 1.89 L
81
rupture of RBC so HB is released from RBC
hemolyzed
82
serum appears yellow due to high bilirubin
icteric
83
serum appears milky or turbid due to high lipid
lipemic
84
Let the blood samples stand for _ minutes at room temperature to allow the blood to clot
Let the blood samples stand for 15 minutes at room temperature to allow the blood to clot
85
Centrifuge blood at _ rpm for _ minutes. Keep container stopper to prevent evaporation.
Centrifuge blood at 2,000-2,500 rpm for 10 minutes. Keep container stopper to prevent evaporation.
86
increase number of formed elements in blood resulting either increase or decrease in plasma volume.
Hemoconcentration
87
Failure of blood to enter the syringe/vacutainer tube
_ pull of plunger
_ the other pole of the vein
_ of vein
Bevel _
Absence of _
Failure of blood to enter the syringe/vacutainer tube
Excessive pull of plunger
Piercing the other pole of the vein
Transfixation of vein
Bevel down
Absence of vacuum
88
Loss of consciousness due to lack oxygen in the brain results in inability to stay in an upright position.
Instructed to breathdeeply
Syncope (fainting)
89
abnormal vascular condition in which thrombosis develops within a blood vessel.
Thrombosis
90
inflammation of a vein often accompanied by a clot which occur as a result of trauma to the vessel wall.
Thrombophlebitis
91
Cortisol
Peaks _ am; lowest _pm-_am;_% lowers at _pm than _am; _ with stress
Peaks 4-6 am; lowest 8pm-12am;50% lowers at 8pm than 8am; increased with stress
92
Adrenocorticotropic hormone (ACTH)
_ at night; _ with stress
Lower at night; Increased with stress
93
Plasma renin activity
_ at night; _ standing than supine
Lower at night; higher standing than supine
94
Aldosterone
_ at night
Lower at night
95
Insulin
_ at night
Lower at night
96
Growth hormone
Higher in _ and _
Higher in afternoon and evening
97
Acid Phosphatase (ACP)
Higher in _ and _
Higher in afternoon and evening
98
Thyroxine
Increases in _
Increases in exercise
99
Prolactin
_ with stress; higher levels at _ and _am and at _ and _pm
Higher with stress; higher levels at 4 and 8am and at 8 and 10pm
100
Iron
Peaks early to late _; decreases up to _% during the day
Peaks early to late morning; decreases up to 30% during the day
101
Calcium
_% decrease supine
4% decrease supine
